Analysis Antioxidant of Fractions Cocoa Beans (Theobroma Cacao L.) as Potential Herbal Medicine

Agung Ari Chandra Wibawa, Dewa Ayu Ika Pramitha, Ni Made Sukma Sanjiwani, Ketut Agus Adrianta

Abstract


The cocoa seeds of the Gumbrih-Bali region are a natural commodity with great potential for development or processing by local communities. The purpose of this research is to provide information about the antioxidant potential contained in cocoa seeds. Testing the antioxidant capacity in this study uses the Uv-Vis spectrophotometry method at 517 nm wavelengths. Free radical scavanger is measured with control absorption and samples was analyzed with regression curve. The research results proved that the antioxidant (AAI) in the n-butanol fraction show result very strong ability of 4.64 compared to the 0.42 ethyl acetate fraction and 0.46 n-hexane fraction. These results prove that the potential chemical content of cocoa seeds is high in polar solvents (n-butanol fractions) such as flavonoid compounds. Furthermore, this research will be used as a basis for developing natural herbal ingredients such as cocoa which can be consumed in the community to prevent degenerative diseases.


Keywords


AAI; antioxidant; cocoa seed; fraction; gumbrih

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References


Akbar, A., Soekamto, N. H., Firdaus, & Bahrun. (2021). Antioxidant of n-hexane, ethyl acetate and methanol extracts of Padina sp with DPPH method. IOP Conference Series: Earth and Environmental Science, 800(1), 012019. https://doi.org/10.1088/1755-1315/800/1/012019

Albano, G. D., Gagliardo, R. P., Montalbano, A. M., & Profita, M. (2022). Overview of the Mechanisms of Oxidative Stress: Impact in Inflammation of the Airway Diseases. Antioxidants, 11(11), 2237. https://doi.org/10.3390/antiox11112237

Baliyan, S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R. P., & Chang, C.-M. (2022). Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules, 27(4), 1326. https://doi.org/10.3390/molecules27041326

Crozier, S. J., Preston, A. G., Hurst, J. W., Payne, M. J., Mann, J., Hainly, L., & Miller, D. L. (2011). Cacao seeds are a “Super Fruit”: A comparative analysis of various fruit powders and products. Chemistry Central Journal, 5(1), 5. https://doi.org/10.1186/1752-153X-5-5

Djebbari, R., Chemam, Y., Amrani, A., Lassed, S., Boubekri, N., Zama, D., Benayache, F., & Benayache, S. (2015). In vitro antioxidant activities of n-butanol extract of Helianthemum confertum. International Journal of Phytomedicine, 7, 119–122.

García-Sánchez, A., Miranda-Díaz, A. G., & Cardona-Muñoz, E. G. (2020). The Role of Oxidative Stress in Physiopathology and Pharmacological Treatment with Pro- and Antioxidant Properties in Chronic Diseases. Oxidative Medicine and Cellular Longevity, 2020, 1–16. https://doi.org/10.1155/2020/2082145

Gulcin, İ., & Alwasel, S. H. (2023). DPPH Radical Scavenging Assay. Processes, 11(8), 2248. https://doi.org/10.3390/pr11082248

Hassanpour, S. H., & Doroudi, A. (2023). Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. 13(4), 354–376.

Jarantow, S. W., Pisors, E. D., & Chiu, M. L. (2023). Introduction to the Use of Linear and Nonlinear Regression Analysis in Quantitative Biological Assays. 3, 1–56. https://doi.org/10.1002/cpz1.801

Kholifah, E., Nurazizah, D., & Noviyanto, F. (2023). Antioxidant Activity and Vitamin C Concentration Analysis of Gandaria (Bouae macrophylla Griff) Ethanol Extract Using Spectrophotometry UV Vis. 3, 54–63. https://doi.org/10.18196/jfaps.v2i1.15992

Kim, S., Lim, S.-W., & Choi, J. (2022). Drug discovery inspired by bioactive small molecules from nature. Animal Cells and Systems, 26(6), 254–265. https://doi.org/10.1080/19768354.2022.2157480

Leyane, T. S., Jere, S. W., & Houreld, N. N. (2022). Oxidative Stress in Ageing and Chronic Degenerative Pathologies: Molecular Mechanisms Involved in Counteracting Oxidative Stress and Chronic Inflammation. International Journal of Molecular Sciences, 23(13), 7273. https://doi.org/10.3390/ijms23137273

Pujiastuti, E., & Andreana, D. (2022). Determination of Total Flavonoid Content of A Peel Ethyl Acetate Extract of Carica papaya L. 1, 58–71.

Rasul, M. G. (2018). Conventional Extraction Methods Use in Medicinal Plants, their Advantages and Disadvantages. 2(6).

Seca, A., & Pinto, D. (2018). Plant Secondary Metabolites as Anticancer Agents: Successes in Clinical Trials and Therapeutic Application. International Journal of Molecular Sciences, 19(1), 263. https://doi.org/10.3390/ijms19010263

Indiarto, R., Pranoto, Y., Santoso, U., & Supriyanto. (2019). In vitro antioxidant activity and profile of polyphenol compounds extracts and their fractions on cacao beans. Pakistan Journal of Biological Sciences, 22(1), 34–44. https://doi.org/10.3923/pjbs.2019.34.44

Jadid, N., Hidayati, D., Hartanti, S. R., Arraniry, B. A., Rachman, R. Y., & Wikanta, W. (2017). Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. Using DPPH assay. 020019. https://doi.org/10.1063/1.4985410

Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023). Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic diseases and aging. Archives of Toxicology, 97(10), 2499–2574. https://doi.org/10.1007/s00204-023-03562-9

Katz, D. L., Doughty, K., & Ali, A. (2011). Cocoa and chocolate in human health and disease. Antioxidants and Redox Signaling, 15(10), 2779–2811. https://doi.org/10.1089/ars.2010.3697

Lokesh, K. N., Channarayappa, Venkataranganna, M., Gunti, G., Patil, H., & Dave, H. (2018). Augmentation of antioxidant and iron (III) chelation properties of tertiary mixture of bioactive ligands. Journal of Trace Elements in Medicine and Biology, 45(October 2017), 114–124. https://doi.org/10.1016/j.jtemb.2017.10.001

Manzano, P., Hernández, J., Quijano-Avilés, M., Barragán, A., Chóez-Guaranda, I., Viteri, R., & Valle, O. (2017). Polyphenols extracted from Theobroma cacao waste and its utility as antioxidant. Emirates Journal of Food and Agriculture, 29(1), 45–50. https://doi.org/10.9755/ejfa.2016-04-388

Njus, D., Kelley, P. M., Tu, Y. J., & Schlegel, H. B. (2020). Ascorbic acid: The chemistry underlying its antioxidant properties. Free Radical Biology and Medicine, 159(May), 37–43. https://doi.org/10.1016/j.freeradbiomed.2020.07.013

Novilda, C. A., & Marcellia, S. (2022). Analisis Senyawa Metabolit Sekunder Ekstrak Metanol Kulit. 11(2), 100–107.

Oracz, J., & Nebesny, E. (2016). Antioxidant Properties of Cocoa Beans (Theobroma cacao L.): Influence of Cultivar and Roasting Conditions. International Journal of Food Properties, 19(6), 1242–1258. https://doi.org/10.1080/10942912.2015.1071840

Oracz, J., & Żyżelewicz, D. (2020). Antioxidants in cocoa. Antioxidants, 9(12), 1–4. https://doi.org/10.3390/antiox9121230

Rohsaita, A., Thohir, M. B., Efendi, M. R. S., Tyas, W. P. K., & Nursaida, M. C. (2024). Synthesis of Colorimetric Sensor for Cyanide Detection with Iron(III) Chloride reagent Using Sol Gel Method with Smarthphone Combination. Vol, 12, 474–486.

Wibawa, A. A. C. (2016). Potensi Flavonoid Ekstrak Biji Mahoni (Swietenia mahagoni Jacq) untuk Menurunkan Konsentrasi 8-OHdG pada Urin Tikus Wistar Jantan yang Terpapar Etanol. Cakra Kimia (Indonesia E-Journal of Applied Chemistry), 4, 146–152.

Wibawa, A. A. C. (2021). Kapasitas Total Antioksidan Ekstrak Metanol Biji Kakao (Theobroma cacao. L) Dengan Metode Spektrofotometri Uv-Vis. Hydrogen: Jurnal Kependidikan Kimia, 9(1), 30. https://doi.org/10.33394/hjkk.v9i1.3794

Winarsih, H. (2007). Antioksidan Alami dan Radikal Bebas. Kanisius, Yogyakarta.

Yusuf, M., Pirman, Nur Fitriani, U. A., Amri, I., & Juwita, A. I. (2021). Identifications of Polyphenols and α-Amylase Inhibitory Activity of Multi herbal Formulation: Cocoa Beans (Theobroma cocoa), Buni (Antidesma bunius L. Spreng) and Cinnamons (Cinnamomum cassia). Journal of Physics: Conference Series, 1783(1). https://doi.org/10.1088/1742-6596/1783/1/012004




DOI: https://doi.org/10.33394/hjkk.v12i4.12548

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