Identification of Antioxidant Activity of Bridelia Micrantha Bark Using the DPPH Method

Faizul Bayani, Baiq Rosmayanti, Ade Sukma Hamdani, Deden Eka Bimaharyanto, Hulyadi Hulyadi

Abstract


This research aims to identify the antioxidant activity of Bridelia micrantha stem bark using the DPPH method. This research is a laboratory experimental research. Antioxidant activity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Melandean Bark Extract is made using the maceration method with 80% methanol solvent. Identification of antioxidant activity was carried out at concentrations of 20 ppm, 40ppm, 60ppm, 80ppm, and 100ppm. Vitamin C was used as a positive control. Absorbance measurements were carried out using a UV-Vis spectrophotometer at a wavelength of 517nm. The test results in this research showed that the IC50 value of melandean bark extract was 85.54 ppm. Melandean bark extract is classified as a strong antioxidant.

Keywords


Bridelia micrantha, antioxidant, DPPH, IC50

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DOI: https://doi.org/10.33394/hjkk.v12i2.11346

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