Determination of Flavonoid Concentration, Phenolic Concentration, and Antioxidant Activity of Allium cepa L Extract

Alfita Dhurrotul Lu'ma, Mirwa Adiprahara Anggarani

Abstract


Free radicals are unstable molecules caused by several electrons in the molecule that does not have a partner. Free radicals can potentially trigger the onset of various degenerative diseases. One of the efforts that can be made to ward off the disease is by consuming antioxidant compounds. Antioxidant compounds can be found in plants with the genus Allium, such as Allium cepa L. This study aims to determine antioxidant activity, flavonoid concentration, and phenolic concentration in Allium cepa L extract. The DPPH method (2-2-diphenyl-1-picryhydrazil) is used to determine antioxidant activity, calorimetrically determined flavonoid consensuality with AlCl3, and the Follin-ciocalteu method is used to determine the phenolic consensual consensuality in the sample. The multilevel maceration method in sample extraction uses different polarity solvents, dichloromethane, ethyl acetate, and 96% ethanol. Based on the research data, flavonoid and phenolic compounds were found in 96% ethanol extract but in dichloromethane and ethyl acetate extracts were not found. The concentration of flavonoid ethanol extract is 96% by 4,125 mgQE/gr extract. Phenolic concentration in ethanol extract was 96% by 1,071 mgGAE/gr extract. The IC50 value in ethanol extract is 96% which is 201,221 ppm. These results show that the antioxidant activity of Allium cepa L is classified as moderate, with IC50 values being between the range of 100-250 ppm.

Keywords


antioxidant activity, Allium cepa L, concentration phenolics, concentration flavonoids

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Andriani, D., & Murtisiwi, L. (2018). Penetapan Kadar Fenolik Konsentarasi Ekstrak Bunga Telang (Clitoria ternatea L.) dengan Spektrofotometri UV-Vis. Cendekia Journal of Pharmacy, 2(1), 32–38. https://doi.org/10.31596/cjp.v2i1.15

Atun, S. (2006). Hubungan Struktur dan Aktivitas Antioksidan Beberapa Senyawa Resveratrol dan Turunannya. Universitas Negeri Yogyakarta.

Cahyaningsih, E., Yuda, P. E. S. K., & Santoso, P. (2019). Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Dengan Metode Spektrofotometri UV-VIS. Jurnal Ilmiah Medicamento, 5(1), 51–57. https://doi.org/10.36733/medicamento.v5i1.851

Dhurhania, C. E., & Novianto, A. (2019). Uji Kandungan Fenolik Konsentarasi dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens). JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA, 5(2), 62. https://doi.org/10.20473/jfiki.v5i22018.62-68

Dontha, S. (2016). A Review on Antioxidant Methods. Asian Journal of Pharmaceutical and Clinical Research, 14. https://doi.org/10.22159/ajpcr.2016.v9s2.13092

Fachriyah, E., Kusrini, D., Haryanto, I. B., Wulandari, S. M. B., Lestari, W. I., & Sumariyah, S. (2020). Phytochemical Test, Determination of Konsentarasi Phenol, Konsentarasi Flavonoids and Antioxidant Activity of Ethanol Extract of Moringa Leaves (Moringa oleifera Lam). Jurnal Kimia Sains Dan Aplikasi, 23(8), 290–294. https://doi.org/10.14710/jksa.23.8.290-294

Firdiyani, F., Agustini, T., W., & Ma’ruf, W., F. (2015). Ekstraksi Senyawa BIoaktif sebagai Antioksidan Alami Spirulina Platensis Segar dengan Pelarut yang Berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(1), 28–37. https://doi.org/10.17844/jphpi.2015.18.1.28

Halliwel, B., & Gutteridge, J. (2007). The chemistry of free radical and related ‘reactive species’ In Free Radical in Biology and Medicine. Oxford University Press.

Harbone, J., B. (1987). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. ITB.

Izzati, N., N., Diniatik, & Rahayu, W., S. (2012). Aktivitas Antioksidan Ekstrak Perasan Daun Manggis (Garcinia mangostana L.) Berdasarkan Metode DPPH (2,2 Diphenyl-1-phycryl hydrazil). Journal of Pharmacy, 9(3), 111–121. https://doi.org/10.30595/pji.v9i3.762

Khaira, K. (2010). Menangkal Radikal Bebas Dengan Antioksidan. Jurnal Saintek, 11(2), 183–187. https://doi.org/10.31958/js.v2i2.28

Kristanti, A., N., Aminah, N., M. ,. T., & Kurniadi, B. (2008). Buku Ajar Fitokimia. Airlangga University Press.

Kuntorini, E., M., Astuti, M., D., & Nugroho, L., H. (2010). Struktur Anatomi dan Aktivitas Antioksidan Bulbus. Berk. Penel. Hayati, 16, 1–7.

Liang, N., & Kitts, D., D. (2014). Antioxidant property of coffee components: Assessment of methods that define mechanisms of action. Molecules, 19(11), 19180–19208. https://doi.org/10.3390/molecules191119180

Marliana, S. D., Suryanti, V., & Suyono, S. (2005). The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.). Biofarmasi Journal of Natural Product Biochemistry, 3(1), 26–31. https://doi.org/10.13057/biofar/f030106

Miguel, C., R. (2017). Phenolic Antioxidant Capacity: A Review of the State of the Art. Intech Open Limited.

Mukhriani, M., Rusdi, M., Arsul, M. I., Sugiarna, R., & Farhan, N. (2019). Kadar Fenolik dan Flavonoid Konsentarasi Ekstrak Etanol Daun Anggur (Vitis vinifera L). ad-Dawaa’ Journal of Pharmaceutical Sciences, 2(2). https://doi.org/10.24252/djps.v2i2.11503

Ness, A., R., & Powles, J., W. (1997). Fruit and vegetables, and cardiovascular disease: A review. International Journal of Epidemiology, 26(1), 1–13. https://doi.org/10.1093/ije/26.1.1

Notoatmodjo, S. (2007). Promosi Kesehatan dan Ilmu Perilaku. Rineka Cipta.

Prasonto, D., Riyanti, E., & Gartika, M. (2017). Uji Aktivitas Antioksidan Ekstrak Bawang Putih (Allium sativum). ODONTO : Dental Journal, 4(2), 122. https://doi.org/10.30659/odj.4.2.122-128

Pratiwi, P., Cahyono, B., & Suzery, M. (2010). Konsentarasi Fenolat dan Flavonoid dari Ekstrak dan Fraksi Daun Kumis Kucing (Orthosiphon stamineus B.) Jawa Tengah Serta Aktivitas Antioksidannya. Jurnal Sains Dan Matematika, 18(4), 140–148.

Pratt, D., E. (1992). Natural Antioxidants from Plant material. American Chemical Society.

Rahayu, S., Kurniasih, N., & Amalia, V. (2015). Ekstraksi dan Identifikasi Senyawa Flavonoid dari Limbah Kulit Bawng Merah Sebagai Antioksidan Alami. al-Kimiya, 2(1), 1–8. https://doi.org/10.15575/ak.v2i1.345

Robinson, T. (1995). Kandungan Organik Tumbuhan Tinggi. ITB.

Runtuwene, M. R., Wewengkang, D., & Julfitriani. (2016). Uji Aktivitas Antioksidan dan Toksisitas Ekstrak Etanol. 5(3), 8.

Salamah, N., & Widyasari, E. (2015). Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) Dengan Metode Penangkapan Radikal 2,2’-difenil-1-pikrihidrazil. Pharmaciana, 5(1), 10.

Samin, A. A., Bialangi, N., & Salimi, Y. K. (2013). Penentuan Kandungan Fenolik Konsentarasi dan Aktivitas Antioksidan Rambut Jagung (ze mays L.) yang Tumbuh di Daerah Gorontalo. Jurnal Saintek, 7(3), 13.

Simanjuntak, K. (2012). Peran Antioksidan Flavonoid Dalam Meningkatkan Kesehatan. 23(3), 6.

Suryani, Y., J., Permana, D., G., & Jambe, A., A. (2016). Pengaruh Jenis Pelarut terhadap Kandungan Konsentarasi Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Matoa (Pometia pinnata). Jurnal Ilmu Dan Teknologi Pangan, 5(1), 1–10.

Syarif, R. A., Muhajir, M., Ahmad, A. R., & Malik, Abd. (2016). Identifikasi Golongan Senyawa Antioksidan Dengan Metode Perendaman Radikal Bebas DPPH Ekstrak Etanol Daun Cordia myxa L. Jurnal Fitofarmaka Indonesia, 2(1), 83–89. https://doi.org/10.33096/jffi.v2i1.184

Tukiran, Miranti, M. G., Dianawati, I., & Sabila, F. I. (2020). Aktivitas Antioksidan Ekstrak Daun Kelor (Moringa oleifera Lam.) dan Buah Bit (Beta vulgaris L.) sebagai Bahan Tambahan Minuman Suplemen. Jurnal Kimia Riset, 5(2), 113. https://doi.org/10.20473/jkr.v5i2.22518

Watson, R., R. (2003). Fungtional Food and Nutraceuticals in Cancer Prevention. Blackwell Publishing.

Werdhasari, A. (2014). Peran Antioksidan Bagi Kesehatan. Jurnal Biotek Medisiana Indonesia, 3(2), 59–68.

Wilujeng, D. T., & Anggarani, M. A. (2021). Penentuan Fenolik Konsentarasi, Flavonoid Konsentarasi, dan Aktivitas Antioksidan Ekstrak Bawang Lanang (Allium Sativm L.). Journal of Chemistry, 10(3), 12.

Wirasti. (2019). Penentapan Kadar Fenolik Konsentarasi, Flavonoid Konsentarasi, dan Uji Aktivitas Antioksidan Ekstrak Daun Benalu Petai (Scurrula atropurpurea Dans.) Beserta Penapisan Fitokimia. Journal of Pharmaceutical and Medicinal Sciences, 4(1), 1–5. https://doi.org/10.32814/jpms.v4i1.73




DOI: https://doi.org/10.33394/j-ps.v10i3.5394

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