Solvent Effects on Extraction Yield and Secondary Metabolites of Melastoma malabathricum Leaf Extract
DOI:
https://doi.org/10.33394/hjkk.v14i5.22137Keywords:
Melastoma malabathricum, Solvent Variation, Extraction Yield, Phytochemical Screening, Secondary MetabolitesAbstract
The selection of extraction solvents and conditions can influence the extraction yield and phytochemical profile of Melastoma malabathricum leaves. However, comparative information on the effects of distilled water, 96% ethanol, and ethyl acetate under their respective extraction conditions remains limited. This study aimed to compare the extraction yield and qualitative secondary metabolite profiles of M. malabathricum leaf extracts obtained using different solvents. Leaf powder was extracted using infusion with distilled water and maceration with 96% ethanol and ethyl acetate. The extraction yield was determined gravimetrically, while qualitative phytochemical screening was performed to identify alkaloids, flavonoids, phenolics, tannins, saponins, triterpenoids, and steroids. The distilled water extract produced the highest yield (9.64%), followed by the 96% ethanol extract (7.96%) and ethyl acetate extract (2.33%). The distilled water extract showed alkaloids, flavonoids, phenolics, tannins, saponins, and triterpenoids, whereas the ethanol extract contained alkaloids, flavonoids, phenolics, tannins, saponins, and steroids. The ethyl acetate extract showed alkaloids, flavonoids, and steroids. These findings demonstrate that the extraction treatments produced different yields and qualitative phytochemical profiles, with distilled water and ethanol showing broader profiles than ethyl acetate. The results provide preliminary comparative information for selecting extraction conditions for M. malabathricum leaves; however, quantitative and instrumental analyses are required to determine metabolite concentrations and chemical identities.
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