Approaches for Spatholobus littoralis: Comparative Efficiency of Ultrasound- and Microwave-Assisted Extraction Techniques and Its Statistical Analysis

Authors

  • Nindya Tri Muliawati IPB University
  • Novia Amalia Sholeha IPB University
  • Mohamad Alief Ramdhan IPB University
  • Farida Laila IPB University
  • Tekad Urip Pambudi Sujarnoko IPB University
  • Wina Yulianti IPB University
  • Atep Dian Supardan IPB University
  • Ika Resmeiliana IPB University
  • Faranita Ratih Listiasari IPB University
  • Hanifa Muthmainna IPB University
  • Khaerunisa Fadillah IPB University
  • Muhammad Afdhal Maliki Akbar IPB University

DOI:

https://doi.org/10.33394/hjkk.v14i3.20353

Keywords:

Ultrasound-Assisted-Extraction, Microwaves, Natural Resources, Spatholobus littoralis

Abstract

The rising demand for sustainable bioactive materials has expedited the advancement of green extraction technologies sourced from renewable natural resources. This research systematically evaluates Microwave-Assisted Extraction (MAE) and Ultrasound-Assisted Extraction (UAE) for the recovery of bioactive compounds from Spatholobus littoralis Hassk (bajakah root) utilising ethanol. The extraction performance was assessed based on yield, total phenolic content, and physicochemical characteristics. The findings indicated that MAE exhibited superior extraction efficiency and enhanced physicochemical properties relative to UAE. The biological functionality of the extracts was assessed using an anti-termite assay. Bajakah root extracts, derived through MAE, were evaluated at concentrations of 1.5% and 2.5%. A commercial termiticide (Permise®) served as a positive control, while 70% ethanol acted as a solvent control, and untreated samples were designated as a negative control. The anti-termite assay demonstrated that both extract concentrations resulted in complete termite mortality within 2 days, while the positive control took up to 5 days and the untreated group required up to 7 days. The findings indicate the efficacy of S. littoralis root extract as a natural bioactive agent and underscore MAE as a reliable and sustainable extraction method for the advancement of multifunctional green materials.

References

Addo, P. W., Gariepy, Y., Shearer, M., Taylor, N., MacPherson, S., Raghavan, V., Orsat, V., & Lefsrud, M. (2024). Microwave-assisted hot air drying of Cannabis sativa: Effect of vacuum and pre-freezing on drying kinetics and quality. Industrial Crops and Products, 218. https://doi.org/10.1016/j.indcrop.2024.119015

Al Jitan, S., Alkhoori, S. A., & Yousef, L. F. (2018). Phenolic Acids From Plants: Extraction and Application to Human Health. In Studies in Natural Products Chemistry (Vol. 58, pp. 389–417). Elsevier B.V. https://doi.org/10.1016/B978-0-444-64056-7.00013-1

Alchera, F., Ginepro, M., & Giacalone, G. (2024). Microwave-assisted extraction (MAE) of bioactive compounds from blueberry by-products using a sugar-based NADES: A novelty in green chemistry. LWT, 192. https://doi.org/10.1016/j.lwt.2023.115642

Avhad, D. N., & Rathod, V. K. (2015). Ultrasound assisted production of a fibrinolytic enzyme in a bioreactor. Ultrasonics Sonochemistry, 22, 257–264. https://doi.org/10.1016/j.ultsonch.2014.04.020

Bansod, S. P., Parikh, J. K., & Sarangi, P. K. (2023). Pineapple peel waste valorization for extraction of bio-active compounds and protein: Microwave assisted method and Box Behnken design optimization. Environmental Research, 221. https://doi.org/10.1016/j.envres.2023.115237

Cheriyan, B. V., Karunakar, K. K., Anandakumar, R., Murugathirumal, A., & kumar, A. S. (2025). Eco-friendly extraction technologies: A comprehensive review of modern green analytical methods. In Sustainable Chemistry for Climate Action (Vol. 6). Elsevier B.V. https://doi.org/10.1016/j.scca.2024.100054

Doulabi, M., Golmakani, M. T., & Ansari, S. (2020). Evaluation and optimization of microwave-assisted extraction of bioactive compounds from eggplant peel by-product. Journal of Food Processing and Preservation, 44(11). https://doi.org/10.1111/jfpp.14853

Hagerman, A. E. (2002). Tannin Chemistry. In Tannin Handbook.

Iskandar, D., & Warsidah, W. (2020). Qualitative Phytochemical Screening and Antioxidant Activity of Ethanol Root Extract of Spatholobus littoralis Hassk. The Journal of Food and Medicinal Plants, 1(1), 13–15. https://doi.org/10.25077/jfmp.1.1.13-15.2020

Jalalzaei, F., Khajeh, M., Ghaffari-Moghaddam, M., & Piri, J. (2025). Comparative evaluation of hybrid LSTM-Based Models for predicting bioactive compound contents and antioxidant activity in microwave-assisted extraction from carrots using natural deep eutectic solvents. LWT, 225. https://doi.org/10.1016/j.lwt.2025.117938

Jaouhari, Y., Bordiga, M., Travaglia, F., Coisson, J. D., Costa-Barbosa, A., Sampaio, P., Botelho, C., Gullón, B., & Ferreira-Santos, P. (2025a). Microwave-assisted extraction of raspberry pomace phenolic compounds, and their bioaccessibility and bioactivity. Food Chemistry, 478. https://doi.org/10.1016/j.foodchem.2025.143641

Jaouhari, Y., Bordiga, M., Travaglia, F., Coisson, J. D., Costa-Barbosa, A., Sampaio, P., Botelho, C., Gullón, B., & Ferreira-Santos, P. (2025b). Microwave-assisted extraction of raspberry pomace phenolic compounds, and their bioaccessibility and bioactivity. Food Chemistry, 478. https://doi.org/10.1016/j.foodchem.2025.143641

Javed, S., Ali, A., & Khanum, T. A. (2021). Biocontrol potential of the entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against the termite species, Microtermes obesi (Holmgren) (Blattodea: Termitidae). Egyptian Journal of Biological Pest Control, 31(1). https://doi.org/10.1186/s41938-021-00448-9

Kristianto, A. H., Siahaan, S. V. B., & Vuspitasari, B. K. (2022). Potensi Pengembangan Ekonomi Sirkular Kerakyatan Dan Solusi Permasalahan Sampah Tidak Terkelola (Studi Kasus Desa Sungai Duri Kabupaten Bengkayang). Jurnal Maneksi, 11(1), 231–236. https://doi.org/10.31959/jm.v11i1.1069

Kumar, R., & Bhaduri, G. A. (2025). Biorefining of pine cone forest waste: Ultrasound assisted extraction followed with thermal degradation for a zero waste process. Industrial Crops and Products, 224. https://doi.org/10.1016/j.indcrop.2024.120278

López, A. R., Ortega-Caneda, E., Espada-Bellido, E., Chinchilla, N., Palma, M., Aliaño-González, M. J., Fernández Barbero, G., & Carrera, C. (2025). Development of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial properties. Food Chemistry, 465. https://doi.org/10.1016/j.foodchem.2024.142006

Marianne, L. C., Lucía, A. G., de Jesús, M. S. M., Eric Leonardo, H. M., & Mendoza-Sánchez, M. (2024). Optimization of the green extraction process of antioxidants derived from grape pomace. Sustainable Chemistry and Pharmacy, 37. https://doi.org/10.1016/j.scp.2023.101396

Martati, E., Stefani, S., & Maisara, K. N. (2025). Ultrasound-assisted extraction optimisation of Bajakah Tampala (Spatholobus littoralis Hassk.) wood using response surface methodology. CYTA - Journal of Food, 23(1). https://doi.org/10.1080/19476337.2025.2566762

Mun, S. P., & Nicholas, D. D. (2017). Effect of proanthocyanidin-rich extracts from pinus radiata bark on termite feeding deterrence. Journal of the Korean Wood Science and Technology, 45(6), 720–727. https://doi.org/10.5658/WOOD.2017.45.6.720

Naznin, M., Alam, M. B., Lee, S. H., & Kim, S. (2024). Optimizing ultrasonic-assisted extraction and untargeted metabolite identification from red water lily (Nymphaea rubra) leaves with enhanced antioxidant activity. Food Chemistry Advances, 4. https://doi.org/10.1016/j.focha.2024.100696

Ngo, K., Castillo, P., Laine, R. A., & Sun, Q. (2021). Effects of menadione on survival, feeding, and tunneling activity of the formosan subterranean termite. Insects, 12(12). https://doi.org/10.3390/insects12121109

Ohmura, W., Doi, S., Aoyama, M., & Ohara, S. (2000a). Antifeedant activity of flavonoids and related compounds against the subterranean termite Coptotermes formosanus Shiraki. J Wood Sci, 46, 149–153.

Ohmura, W., Doi, S., Aoyama, M., & Ohara, S. (2000b). Antifeedant activity of flavonoids and related compounds against the subterranean termite Coptotermes formosanus Shiraki.

Peron, G., Bernabé, G., Marcheluzzo, S., Zengin, G., Ibrahime Sinan, K., Hošek, J., Treml, J., Kaja, I., Paccagnella, M., Brun, P., Castagliuolo, I., Zancato, M., & Dall’Acqua, S. (2024). Orange fruit peels from PDO varieties of Ribera (Sicily, Italy): An insight into the chemistry and bioactivity of volatile and non-volatile secondary metabolites extracted using a microwave-assisted method. Journal of Functional Foods, 116. https://doi.org/10.1016/j.jff.2024.106147

Plaza, M., & Marina, M. L. (2025). Natural deep eutectic solvents and ultrasound-assisted extraction for the recovery of antioxidant phenolic compounds from orange pomace. Microchemical Journal, 212. https://doi.org/10.1016/j.microc.2025.113366

Prasetyaningrum, A., Jos, B., Ratnawati, R., Rokhati, N., Riyanto, T., & Prinanda, G. R. (2022). Sequential Microwave-Ultrasound Assisted Extraction of Flavonoid from Moringa oleifera: Product Characteristic, Antioxidant and Antibacterial Activity. Indonesian Journal of Chemistry, 22(2), 303–316. https://doi.org/10.22146/ijc.65252

Prevete, G., Carvalho, L. G., del Carmen Razola-Diaz, M., Verardo, V., Mancini, G., Fiore, A., & Mazzonna, M. (2024). Ultrasound assisted extraction and liposome encapsulation of olive leaves and orange peels: How to transform biomass waste into valuable resources with antimicrobial activity. Ultrasonics Sonochemistry, 102. https://doi.org/10.1016/j.ultsonch.2024.106765

Rahman, Y., & Ismanto, A. (2022). Komposisi Kimia, Karakteristik Fisik Dan Nilai Organoleptik Nugget Itik Manila (Cairinamoschata) Yang Diberi Pakan Limbah Pasar Samarinda. Jurnal Peternakan Lingkungan Tropis, 3(2), 94. https://doi.org/10.30872/jpltrop.v3i2.6858

Ramadhan, I., & Sujono, T. A. (2025). Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Akar Bajakah (Spatholobus littoralis Hassk) pada Tikus Putih (Rattus norvegicus) yang Diinduksi Aloksan. Usadha Journal of Pharmacy, 4(1), 102–115. https://doi.org/10.23917/ujp.v4i2.550

Santos-Martín, M., Cubero-Cardoso, J., González-Domínguez, R., Cortés-Triviño, E., Sayago, A., Urbano, J., & Fernández-Recamales, Á. (2023). Ultrasound-assisted extraction of phenolic compounds from blueberry leaves using natural deep eutectic solvents (NADES) for the valorization of agrifood wastes. Biomass and Bioenergy, 175. https://doi.org/10.1016/j.biombioe.2023.106882

Sujarnoko, T. U. P., Ridwan, R., Nahrowi, & Jayanegara, A. (2020). Extraction of Tannin from Acacia (Acacia mangium) Bark and its use as a Feed Additive for Protecting in vitro Ruminal Degradation of Tofu Dregs. Advances in Animal and Veterinary Sciences, 8(7), 761–765. https://doi.org/10.17582/journal.aavs/2020/8.7.761.765

Sun, S., Yu, Y., Jo, Y., Han, J. H., Xue, Y., Cho, M., Bae, S. J., Ryu, D., Park, W., Ha, K. T., & Zhuang, S. (2025). Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures. In Frontiers in Pharmacology (Vol. 16). Frontiers Media SA. https://doi.org/10.3389/fphar.2025.1615338

Supardan, A. D., Nuraini, F., Ramdhan, M. A., Sholeha, N. A., bin Mohd Azami, M. S., Listiasari, F. R., Muliawati, N. T., Laila, F., Resmeiliana, I., & Pambudi Sujamoko, T. U. (2024). Impact of temperature and duration variations on the quality of acacia bark tannin for in-vitro treatment of sheep diarrhoea. E3S Web of Conferences, 577. https://doi.org/10.1051/e3sconf/202457702017

Taşkın, B., & Aksoylu Özbek, Z. (2020). Optimisation of microwave effect on bioactives contents and colour attributes of aqueous green tea extracts by central composite design. Journal of Food Measurement and Characterization, 14(4), 2240–2252. https://doi.org/10.1007/s11694-020-00471-8

Teknik Kimia, D., S Augustia, V. A., Charfadz, N., & Akbar, R. (2021). JURNAL TEKNIK KIMIA-USU Pengaruh Waktu Ekstraksi, Rasio Bahan/Pelarut, dan Daya Microwave Terhadap Hasil Ekstraksi Minyak Serai Dapur dengan Bantuan Gelombang Mikro Effect of Extraction Time, Ingredient/Solvent Ratio, and Microwave Power on Extraction Results of Lemongrass Oil with Microwave Assistance. Jurnal Teknik Kimia USU, 10(2). https://talenta.usu.ac.id/jtk

Usman, M., Nakagawa, M., & Cheng, S. (2023). Emerging Trends in Green Extraction Techniques for Bioactive Natural Products. In Processes (Vol. 11, Number 12). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/pr11123444

Yuniarto, K., Mustiko Okta Muvianto, C., & Studi Teknik Pertanian -Fakultas Teknologi Pangan dan Agroindustri, P. (2021). Aplikasi Ultrasound Assisted Extraction untuk Produksi Minyak Bawang Putih Varietas Lokal. In Jurnal Teknologi Pertanian (Vol. 22, Number 3).

Yuniati, Y., Gala, S., Sumarno, S., & Mahfud, M. (2025). Investigation of the Extraction Parameters on Acquisition of Natural Colorants from Coleus atropurpureus L. Benth Leaves Using Microwave-and Ultrasonic-Assisted Extraction. Indonesian Food Science and Technology Journal, 8(2), 253–263. https://doi.org/10.22437/ifstj.v8i2.42509

Zhang, S., Xie, H., Huang, J., Chen, Q., Li, X., Chen, X., Liang, J., & Wang, L. (2024). Ultrasound-assisted extraction of polyphenols from pine needles (Pinus elliottii): Comprehensive insights from RSM optimization, antioxidant activity, UHPLC-Q-Exactive Orbitrap MS/MS analysis and kinetic model. Ultrasonics Sonochemistry, 102. https://doi.org/10.1016/j.ultsonch.2023.106742

Published

2026-06-30

How to Cite

Muliawati, N. T., Sholeha, N. A., Ramdhan, M. A., Laila, F., Sujarnoko, T. U. P., Yulianti, W., … Akbar, M. A. M. (2026). Approaches for Spatholobus littoralis: Comparative Efficiency of Ultrasound- and Microwave-Assisted Extraction Techniques and Its Statistical Analysis. Hydrogen: Jurnal Kependidikan Kimia, 14(3). https://doi.org/10.33394/hjkk.v14i3.20353

Issue

Section

Articles