Antioxidant Activity of Hydnophytum formicarum Extract by TEAC method (Trolox Equivalent Antioxidant Capacity Assay)

Mayshah Purnamasari

Abstract


Oxidative stress, caused by excess reactive oxygen species (ROS), is believed to be a major contributor to aging and various degenerative diseases. Plant-based antioxidants have gained significant attention for their potential in combating R.O.S. Hydnophytum formicarum, traditionally used to treat diabetes, is evaluated as a natural antioxidant source. This study is the first to assess the antioxidant activity of H. formicarum tuber ethanol extract using the TEAC method, revealing a high antioxidant capacity (220.38 ± 0.61 M TE/100 g), highlighting its potential as a dietary supplement.

Keywords


Hydnopytum formicarium, antioxidants, TEAC, ABTS

Full Text:

PDF

References


Abdullah, N. S., Ahmad, W. Y. W., & Sabri, N. A. (2017). Juzuk kimia daripada tuber muda Hydnophytum formicarum. Malaysian Journal of Analytical Sciences, 21(2), 291–297. https://doi.org/10.17576/mjas-2017-2102-03

Amir, M., Ullu, A., & Kusmiati, D. (2020). Uji Aktivitas Antioksidan Tanaman Sarang Semut (Hydnophytum formicarum Jack) dengan Metode ABTS dan Identifikasi Senyawa Aktif Menggunakan LC-MS Antioxidant Activity of “Sarang semut” (Hydnophytum formicarum Jack) with ABTS Method and Identification of Active Compound Using LC-MS. In Archives Pharmacia ISSN (Vol. 2, Issue 1).

Ananda, N., Ariawan, D., & Juniantito, V. (2022). Effects of the Hydnophytum formicarum plant extract on collagen density, angiogenesis, wound length, and re-epithelialization in wound healing: Experimental study on rats. Dental and Medical Problems, 59(1), 67–73. https://doi.org/10.17219/dmp/140208

Andriani, D., & Murtisiwi, L. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol 70% Bunga Telang (Clitoria ternatea L) dari Daerah Sleman dengan Metode DPPH Antioxidant Activity Test of 70% Ethanol Extract of Telang Flower (Clitoria ternatea L) from Sleman Area with DPPH Method. In Jurnal Farmasi Indonesia (Vol. 1, Issue 1). http://journals.ums.ac.id/index.php/pharmacon

Andriani, Y., Mohamad, H., Kassim, M. N. I., Rosnan, N. D., Syamsumir, D. F., Saidin, J., Muhammad, T. S. T., & Amir, H. (2017). Evaluation on Hydnophytum formicarum tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for antibacterial and antioxidant activities, and anti-cancer potency against MCF-7 and HeLa cells. Journal of Applied Pharmaceutical Science, 7(9), 30–37. https://doi.org/10.7324/JAPS.2017.70904

Darwis, D., Hertiani, T., & Samito, E. (2014). The effects of Hydnophytum formicarum ethanolic extract towards lymphocyte, vero and T47d cells proliferation in vitro. Journal of Applied Pharmaceutical Science, 4(6), 103–109. https://doi.org/10.7324/JAPS.2014.40616

Dhurhania, C. E., & Novianto, A. (2018). Uji Kandungan Fenolik Total dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens). Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 5(2), 62.

Hertiani, T., & Pratiwi, S. U. T. (2015). Hydnophytum formicarum Jack ethanol extract modulates quorum sensing-controlled pathogenicity in Pseudomonas aeruginosa. Pakistan Journal of Pharmaceutical Sciences, 28(5), 1691–1697.

Keawchai, K., Chumkaew, P., Permpoonpattana, P., & Srisawat, T. (2022). Synergistic effect of Hydnophytum formicarum tuber and Vatica diospyroides Symington cotyledon extracts with ampicillin on pathogenic bacteria. Journal of Applied Biology and Biotechnology, 10(2), 6–11. https://doi.org/10.7324/JABB.2022.100202

Khalidah, A. R. (2020). Literature Review: Mekanisme Resistensi Kemoterapi Berbasis Platinum Literature Review: Mechanism Resistance of Platinum-Based Chemotherapy. In Jurnal Kesehatan (Vol. 11, Issue 1). Online. http://ejurnal.poltekkes-tjk.ac.id/index.php/JK

Khasanah, N. W., Karyadi, B., & Sundaryono, A. (2020). Uji Fitokimia dan Toksisitas Ekstrak Umbi Hydnophytum sp. terhadap Artemia salina Leach. PENDIPA Journal of Science Education, 4(1), 47–53. https://doi.org/10.33369/pendipa.4.1.47-53

Lin, C. Y., Tam Ly, M., Yang, S. H., Lai, S. C., Chang, T. W., Lin, I. H., & Tzeng, Y. J. (2021). Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells. Natural Product Communications, 16(10). https://doi.org/10.1177/1934578X211050521

Nugraha, A. S., Wangchuk, T., Willis, A. C., Haritakun, R., Sujadmiko, H., & Keller, P. A. (2019). Phytochemical and pharmacological studies on four Indonesian epiphytic medicinal plants: Drynaria rigidula, Hydnophytum formicarum, Usnea misaminensis, and Calymperes schmidtii. Natural Product Communications, 14(6). https://doi.org/10.1177/1934578X19856792

Nurwaini, S., Tasya Amanda, A., & Jl Ahmad Yani Kartasura, S. (2024). Formulation And Evaluation Of Telang Flower (Clitoria Ternatea L.) Extract Gel Preparation With Tragacanth As Gelling Agent And Antioxidant Activity Test. In Jurnal Farmasi Indonesia (Vol. 21, Issue 1). http://journals.ums.ac.id/index.php/pharmacon

Prachayasittikul, S., Buraparuangsang, P., Worachartcheewan, A., Isarankura-Na-Ayudhya, C., Ruchirawat, S., & Prachayasittikul, V. (2008). Antimicrobial and Antioxidative Activities of Bioactive Constituents from Hydnophytum formicarum Jack. Molecules, 13, 904–921. www.mdpi.org/molecules

Prajuabjinda, O., Panthong Bsc, S., & Itharat Phd, A. (n.d.). Antimicrobial Activity of Thai Medicinal Preparation of Khampramong Temple Used for Cancer Treatment and Its Plant Components. In J Med Assoc Thai (Vol. 95). http://jmat.mat.or.th

Prayong, P., Barusrux, S., & Weerapreeyakul, N. (2008). Cytotoxic activity screening of some indigenous Thai plants. Fitoterapia, 79(7–8), 598–601. https://doi.org/10.1016/j.fitote.2008.06.007

Rachpirom, M., Barrows, L. R., Thengyai, S., Ovatlarnporn, C., Sontimuang, C., Thiantongin, P., Puttarak, P., Rachpirom, M., & Louis, R. (2022). Antidiabetic Activities of Medicinal Plants i n Traditional Recipes and Candidate Antidiabetic Compounds from Hydnophytum formicarum Jack . Tubers. 14(1), 89–99.

Sababathy, M., Nur Islamiah Kassim, M., Shamsudin Ahmad, A., Amir, H., Fitrya Syamsumir, D., & Andriani, Y. (2020a). Phytochemicals Study, Antioxidant and Cytotoxicity Properties of Hydnophytum formicarum (Kepala beruk) Leaves against HepG2 and HeLa Cell Lines. Oriental Journal of Chemistry, 36(03), 425–433. https://doi.org/10.13005/ojc/360310

Sada, E., Siburian, R. H. S., & Panambe, N. (2018). Ekologi Tempat Tumbuh Sarang Semut Pada Taman Wisata Alam Gunung Meja Manokwari. EnviroScienteae, 14(3), 187. https://doi.org/10.20527/es.v14i3.5690

Safitri, R. A., Saptarini, O., & Sunarni, T. (2020). Cytotoxic Activity , Expression of p53 , and Bcl-2 Extract Fraction of Kelakai Herbs ( S. September.

Safniyeti, S., Sulistijorini, S., & Chikmawati, T. (2018). Species Richness and Habitat Suitability of Myrmecophytes in Bengkulu : Host Tree, Coexist Epiphytes and Animals. Biosaintifika: Journal of Biology & Biology Education, 10(1), 183–190. https://doi.org/10.15294/biosaintifika.v10i1.13025

Seeram, N. P., Henning, S. M., Niu, Y., Lee, R., Scheuller, H. S., & Heber, D. (2006). Catechin and caffeine content of green tea dietary supplements and correlation with antioxidant capacity. Journal of Agricultural and Food Chemistry, 54(5), 1599–1603. https://doi.org/10.1021/jf052857r

Shahrudin, R., Shukor Yusoff, A., Fahmi Kamaruzzaman, M., & Razali Salam, M. (2017). Distribution and Host Identification of Epiphytic Plant, Hydnophytum formicarum Jack, in Pulau Telaga Tujuh, Setiu, Terengganu. Journal of Sustainability Science and Management, 12, 128–134.

Velanita1, S., Ismardianita, E., & Pascawinata, A. (2019). The effect of ant-plant (Hydnophytum formicarum) ethanol extract on collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya). Padjadjaran Journal of Dentistry, 31(3), 189–195. https://doi.org/10.24198/pjd.vol31no1.19328

Vintisen, R., Krishnan, K. T., Veloo, K. V., Rajandas, H., Parimannan, S., & Ravichandran, M. (2024). Gas chromatography-mass spectrometry (GC-MS) analysis of phytocompounds isolated from Hydnophytum formicarum Jack tuber extracts. Journal of Tropical Resources and Sustainable Science (JTRSS), 12(1), 36–46. https://doi.org/10.47253/jtrss.v12i1.1363

Widodo, H., Sismindari, Asmara, W., & Rohman, A. (2020). Antioxidant activities of methanolic extract and its fractions of Baccaurea racemosa and Macaranga subpeltata leaves. Food Research, 4(1), 127–134. https://doi.org/10.26656/fr.2017.4(1).144

Widodo, H., Sismindari, S., Asmara, W., & Rohman, A. (2019). Antioxidant activity, total phenolic and flavonoid contents of selected medicinal plants used for liver diseases and its classification with chemometrics. Journal of Applied Pharmaceutical Science, 9(6), 99–105. https://doi.org/10.7324/JAPS.2019.90614

World Health Organization. (2022). Cancer. https://www.who.int/en/news-room/fact-sheets/detail/cancer

Yusoff, A. S., Wan Omar, W. B., & Rohanf, S. (2020). Limited seed dispersal may shape genetic structure of Hydnophytum formicarum jack populations in mangrove ecosystem. Biotropia, 27(2), 153–161. https://doi.org/10.11598/BTB.2020.27.2.1198




DOI: https://doi.org/10.33394/hjkk.v12i5.12624

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.