Penghambatan Xantin Oksidase Oleh Gel Ekstrak Takokak (Solanum torvum Swartz) Berbasis Nanoteknologi Secara In Vitro

Muhammad Imam Dwiyansyah, Rania Insyira, Almira Maurilla Mirsa, Azzara Gita Sawalia, Daffa Nugra, Salmi Salmi

Abstract


Inhibition of xanthine oxidase is one of the strategies in handling gout. This study aims to explore the potential of takokak fruit extract (Solanum torvum Swartz) in inhibiting nanotechnology-based xanthine oxidase in the form of nanoemulgel. This research is an experimental study conducted in several stages, namely qualitative phytochemical tests, quantitative identification of total flavonoid content and preparation of nanoemulgel made with 3.75% extract with variations of surfactant Tween 80 and PEG 400, namely F1 (30:30), F2 (35:25) and F3 (40:20). The ability of the extract and nanoemulgel was evaluated in inhibiting xanthine oxidase. The results showed that the ethanol extract of takokak fruit contained flavonoids, saponins, phenolics and tannins qualitatively. The total flavonoid content in the extract was 4.472 ± 0.133 mgQE. Formulation F2 has the smallest particle size (18.5 nm) and polydispersity index of 0.1313. Takokak fruit extract has almost the same inhibitory power as allupurinol with an IC50 value of 45.54 ppm. The IC50 value of nanoemulgel 3.75% takokak extract is 29.26 ppm so that the IC50 of the extract in nanoemulgel form is 1.10 ppm (45 times the IC50 of the crude extract). In conclusion, Formula F2 is the best formula in the preparation of nanoemulgel with particle size and polydispersion index and strong xanthin oxidase inhibitory activity.

Keywords


takokak, Solanum torvum Swartz, nanoemulgel, xanthine oxidase

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References


[GBD] Global Burden of Diseases, Injuries and Risk Factors Study 2021.(2024). Global, regional and national burden of gout, 1990-2020, and projection to 2050: a systematic analysis of the global burden of disease study 2021. Lancet Rheumatoid, 6:e507-517.

[SNI] Standar Nasional Indonesia. (1996). SNI 16-4399-1996 ICS Standar SNI tentang Kosmetik. Dewan Standarisasi Nasional: Jakarta.

Abubakar, A.R. & Hague, M. (2020). Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes. J. Pharm. Bioalled Sci. 12(1): 1-10.

Acharyya, S. & Babli, K. (2018). Antimicrobial and analgesic activity of Solanum torvum. The Saudi Journal of Life Sciences.3(6):459-464.

Aithal, G.C., Narayan, R., Nayak, U.Y. (2020). Nanoemulgel: a promising phase in drug delivery. Curr. Pharm. 26: 279

Aliwu, I., Rorong, J. A., & Suryanto, E. (2020). Skrining fitokimia dan uji efek sedatif pelarut dari daun takokak (Solanum torvum Swartz) pada tikus putih galur wistar. Chemical Program. 13(1):6-10

Alkilani, A.Z., McCrudden, M.T.C., & Donnelly, R.F. (2015). Transdermal drug delivery: innovative pharmaceutical developments based on disruption of the barrier properties of stratum corneum. Pharmaceutics, 7: 438-470.

Andini,S., Yulianita & Febriani, E.N.K. (2023). Formulasi sediaan nanoemulgel ekstrak buah lada hitam (Piper ningrum L.) dengan variasi konsentrasi Tween 80 dan PEG400. Majalah Farmasetika. 8(3):250-266

Anggriani R., Putri, N.N.P., & Wahyudi, V.A. (2023). Study of physical, chemical and Organoleptic Properties of Functional Drink Turkey Berry (Solanum torvum Swartz) with the Addition of Butterfly Pea Flower (Clitoria ternatea linn) and Emprit Ginger (Zingiber officinale var. Amarum). Biology, Medicine & Natural Product Chemistry. 12(2): 563-575.

Anwar, H.U., Andarwulan, N., & Yuliana, N.D. (2017). Identifikasi komponen antibakteri pada ekstrak buah takoakk menggunakan kromatografi lapis tipis. Jurnal Mutu Pangan. 4(2): 59-64.

Cha, Y., Lee, J., Choy, W., Lee, J.S., Lee, H.H.,& Chae, D.S. (2024). Pathophysiology and treatment of gout arthritis: including gout arthritis of hip joint: a literature review. Hip & Pelvis, 36(1): 1-11.

Clebak, K.T., Morrison, A., Croad, J.R. 2020. Gout; rapid evidance review. Am. Fam. Physician. 102:533-538

Da Silva, S.L., Da Silva, A., Honorio, K.M., Marangoni, S., Toyama, M.H., & Da Silva, A.B.F. (2004). The influence of electronic, steric and hydrophobic properties of flavonoid compounds in the inhibition of the xanthine oxidase. Journal of Molecular Structure: THEOCHEM. 684(1-3):1-7.

Donthi, M.R., Munnangi, S.R., Krishna, K.V., Saha, R.N., Singhvi, G., Dubey, S.K. (2023). Nanoemulgel: a novel nano carrier as atool for topical drug delivery. Pharmaceutics. 15: 164.

Dwivedi, K., Mandal., A.K., Afzal, O., Altamimi, A.S.A., Sahoo, A., Alossaimi, M.A. et al. (2023). Emerge of nano-based formulation for effective delivery of flavonoids againts topical infectious disorders. Gels. 9:671.

Fitriana.(2015). Penggunaan allopurinol dalam jangka panjang: risiko dan alternatif pengobatan. Jurnal Kesehatan Alternatif. 3(2):55-62.

Gohil, D., Panchal, D., Saggu, V., Sajan, C., Joshi, K., Patel, K., et al., (2023). Transdermal drug delivery system (TDDS): an overview. Research Trends in Science and Technology. 1: 45-52

Hamzah, F.N., Subandi, Sujarwo, W., Septama, A.W., & Mozef, T. (2020). Antioxidant and xanthine oxidase inhibitory activities of Kecapi (Sandoricum koetjape (Burm.f) Merr.) leaf extract. IOP conf. Series: Materials Sciences and Engineering. 833:012012.

Han T & Das D.B., (2015). Potential of combined ultrasound and microneedles for enhanced transdermal drug permetion: a review. Eur. J. Pharm Biopharm, 89. 312-328.

Harborne. (1987). Metode Fitokimia: Penuntun cara modern menganalisis tumbuhan. Edisi I. Terjemahan Kosasih Padmawinata dan Iwang Soediro. Bandung: Penerbit ITB

Hasin, A., & Rachmadana, Z. (2019). Analisis kadar kalsium oksalat (CaC2O4) pada daun dan batang tanaman bayam di pasar tradisional kota Makassar. Jurnal Media Laboran. 9(1): 6–11.

Huang, H., Zhu, Y., Fu, X., Zou, Y., Li, Q., & Lou, Z. (2022). Integrated natural deep eutectic solvent and puls-ultrasonication for effecient extraction of crocins from gardenia fruits (Gardenia jasminoides Ellis) and its bioactivities. Food Chemistry. 380:132216.

Hui, X., Meng, X., Demine, G., & Gouwen, Z. (2023). Mechanism of flavonoids inhibiting xanthine oxidase and alleviating hyperuricemia from structure-activity relationship and animal experiment: A review. Food Frontier. 4: 1643-1665.

Imran M, Haya, M, Mohammad, A. K., & Abdul, Q. (2022). Phytochemical screening of solanum xanthocarpum and its xanthine oxidase inhibitory activity. Journal of Medical, Pharmaceutical, and life Sciences. 02(03): 311-323.

Kaur, R., & Ajitha, M. (2019). Transdermal delivery of fluvastatin loaded nanoemulsion gel: Preparation, characterization and in vivo anti-osteoporosis activity. European Journal of Pharmaceutical Sciences, 136(5): 1099-1126.

Kementerian Kesehatan Republik Indonesia (Kemenkes RI).(2013). Hasil Utama Riset Kesehatan Dasar (Riskesdas) 2013. Diunduh di https://repository.badankebijakan.kemkes.go.id/id/eprint/4467/1/Laporan_riskesdas_2013_final.pdf. tanggal 10 Februari 2024

Kementerian Kesehatan Republik Indonesia (Kemenkes RI).(2018). Hasil Utama Riset Kesehatan Dasar (Riskesdas) 2018. Diunduh di https://kesmas.kemkes.go.id/assets/upload/dir_519d41d8cd98f00/files/Hasil-riskesdas-2018_1274.pdf tanggal 10 Februari 2024

Kim, Y., Oh, H.C., Park, J.W., Kim, I.S., Kim, J.Y., Kim, K.C. et al. (2017). Diagnosis and treatment of inflammatory joint disease. Hip Pelvis. 29(4): 211-222.

Lajira, M. M., & Lister, I.N.E. (2019). Uji antibakteri ekstrak buah takokak (Solanum torvum Swartz) terhadap pertumbuhan bakteri Propionibacterium acnes. BioLink: Jurnal Biologi Lingkungan, Industri dan Kesehatan.6(1). 73-79

Latourte, A., Dumurgier, J., Paquet, C., & Richette, P. (2021). Hyperuricemia, gout, and the brain—an update. Current Rheumatology Reports, 23(12), 82.

Liga, S., Paul, C., & Peter, F. (2023). Flavonoids: overview of biosynthesis, biological activity and current extraction techniques. Plants. 12: 2732.

Mailuhu M., Runtuwenw M.R.J., & Koleangan H.S.J. (2017). Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Kulit Batang Soyogik (Saurauia bracteosa DC). Chem.Prog.10 (1): 1-6

Maiuolo, J., Oppedisano, F., Gratteri, S., Muscoli, C., & Mollace, V. (2016). Regulation of uric acid metabolism and excretion. International Journal of Cardiology.213:8-14.

Mappa, T., Edy, H. J., & Kojong N. (2013). Formulasi gel ekstrak daun sasaladahan (Peperomia Pellucida (L.) H.B.K) dan uji efektivitasnya terhadap luka bakar pada kelinci (Oryctolagus Cuniculus). Jurnal Ilmiah Farmasi UNSRAT. 2(02):2302-2493.

Nguyen, D.H., Liu, T.W., Hsu, S.J., Huynh, Q.D.T., Doung, T.L.T., Chu, M.H., et al. (2024). Xanthine oxidase inhibition study of isolated secondary metabolites Dolichandrone spathacea (bignoniacease): in vitro and in silico approach. Saudi Pharmaceutical Journal. 32:101980.

Nurjannah. (2024). Potensi Antibakteri Daun Pelawan (Tristaniopsis Merguensis Griff.) Terhadap Bakteri Cutibacterium Acnes, Staphylococcus Aures, Dan Staphylococcus Epidermidis Penyebab Jerawat. Universitas Bangka Belitung.

Ou, R., Lin, L., Zhao, M., & Xie, Z. (2020). Action mechanisms and interaction of two key xanthine oxidase inhibitor in galangal: combination on in vitro and in silico molecular docking studies. International Journal of Biological Macromolecules. 162:1562-1535.

Pohan, E. (2019). FOrmulasi dan uji stabilitas krim ekstrak methanol daun beluntas (Pluchea indica L.) dari kota Benteng Kabupaten Kepulauan Selayar Provinsi Sulawesi Selatan. Jurnal Farmasi Sandi Karsa. 5(1): 57–64.

Purwandari, V., Sianipar, A.Y., Silalahi, Y.C.E., & Nasution, D.J. (2020). Uji efektivitas antibakteri nano gel bahan aktif ekstrak kayu manis (Cinnamomum burmannii) terhadap Staphylococcus aureus. Farmanesia. 7(2): 92-98.

Qomaliyah, E. N., Indriani, N., Rohma, A., & Islamiyati, R. (2023). Skrining fitokimia, kadar total flavonoid, dan antioksidan daun cocor bebek. Curr. Biochem. 10(1): 1-10.

Qurie, A., Preuss, C.V., Musa, R.(2023). Allopurinol. StatPerals[internel]. Diunduh di https://www.ncbi.nlm.nih.gov/books/NBK499942/ tanggal 28 Juli 2024

Rasul, M.G. (2018). Contional extraction methods use in medicinal plants, theor advantages and disadvantages. International Journal of Basic Scineces and Applied Computing. 2(6): 10-14.

Rini, H., Elin Y.S., Kusnandar, A., & Sukrasno. (2016). In Vitro evaluation of xanthine oxidase inhibitory activity of selected medicinal plants. International Journal of Pharmaceutical and Clinical Research. 8(4): 235-238.

Suyani, H.,(1991). Kimia dan Sumber Daya Alam, Padang: Pusat Penelitian Universitas Andalas.

Tjitraresmi, A., Wicaksono, I.A., Henriani, R., Susilawati, Y., Noviandri, I., Wulandari, E.D., Ivan, N.H. (2023). Screening for anti-hyperuricemia potential of some indonesian medicinal plants through xanthine oxidase inhibition in vitro assay. International Journal of Applied Pharmaceutics. 15(2): 113-117.

Wang, T.Y., Li, Q., & Bi, K.S. (2018). Bioactive flavonoids in medicinal plants structure, activity and biological fate. Asian Journal of Pharmaceutical Sciences. 13:12-23.

Wang, W., Zhou, H., Liu, L. (2018). Side effects of methotrexate therapy for rheumatoid arthritis: a systematic review. Eur. J. Med. Chem. 158: 502-516.

Wibowo, S.A., Budiman, A., & Hartanti, D. (2017). Formulasi dan aktivitas antijamur sediaan krim M/A ekstrak etanol buah takokak (Solanum torvum Swarts) terhadap candida albicans. Jurnal Riset Sains dan Teknologi. 1(1): 15-21.

Xue, H., Xu, M., Gong, D. & Zhang, G. (2023). Mechanism of flavonoids inhibiting xanthine oxidase and alleviating hyperuricemia from structure-activity relationship and animal experiments: a review. Food Frontiers. 4: 1643-1665.




DOI: https://doi.org/10.33394/bioscientist.v12i2.12610

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