Uji Aktivitas Antibakteri Ekstrak Metanol Propolis dari Lebah Kelulut (Heterotrigona itama) terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus

Dyah Tantri Wulandari, Supomo Supomo, Husnul Warnida

Abstract


One of the causes of infection is influenced by the occurrence of wounds. Treatment of infected wounds using antibiotics. But antibiotics causes resistance. Then another alternative material from natural ingredients is used, is Propolis from Heterotrigona itama bees. This study aims to determine the inhibition, MIC and MBC of Propolis methanol extract with concentrations of 10%,20%30%,40%,50% against Pseudomonas aeruginosa and Staphylococcus aureus. Research uses experimental research with a non-probability sampling technique, purposive sampling. The extraction method uses methanol solvent maceration. Antibacterial test was carried out by disc diffusion method, positive control of 0.1% clindamycin, negative control of DMSO. The independent variable is the concentration of propolis methanol extract and the dependent variable is the inhibition zone formed and the MIC and MBC values. Analyzed statistically using the ANOVA. Propolis methanol extract inhibited Pseudomonas aeruginosa bacteria at concentrations of 10%:7.74mm, 20%:8.05mm, 30%:10.00mm, 40%:10.05mm, 50%:12,25mm, and can inhibit the growth of Staphylococcus aureus bacteria at concentrations of 10%:7,05mm, 20%:8.00mm, 30%:10.20mm, 40%:11.15mm, 50%:12,05mm. MIC and MBC methanol extract of propolis on the bacteria Pseudomonas aeruginosa and Staphylococcus aureus of 100 mg/ml. Statistical test results showed that all concentrations of propolis methanol extract had a significant difference with the positive control of Clindamycin 0.1%.

Keywords


Pseudomonas aeruginosa, Staphylococcus aureus, Propolis, Heterotrigona itama, Antibacterial Activity.

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References


Achyani., & Wicandra, D. (2019). Kiat Praktis Budidaya Lebah Trigona (Heterotrigona itama). Lampung: CV. Laduny Alifatama.

Anisa, N., & Najib, S. Z. (2022). Skrining Fitokimia dan Penetapan Kadar Total Fenol Flavonoid dan Tanin pada Daun Kersen (Muntingia calabura L.). Indonesian Journal Pharmaceutical and Herbal Medicine, 2(1), 96-104.

Campos, J. F., dos Santos, U. P., da Rocha, P. d. S., Damião, M. J., Balestieri, J. B. P., Cardoso, C. A. L., Gamero, E. J. P., Estevinho, L. M., Souza, K. d. P., & dos Santos, E. L. (2015). Antimicrobial, Antioxidant, Antiinflammatory, and Cytotoxic Activities of Propolis from the Stingless Bee Tetragonisca fiebrigi (jataí). Evidence-Based Complementary and Alternative Medicine, (2015), 1-11. https://doi.org/10.1155/2015/296186

Cankaya, I. I. T., & Somuncuoglu, E. I. (2021). Review Article : Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents Against Respiratory Tract Infections. Evidence-Based Complementary and Alternative Medicine, (2021), 1-14. https://doi.org/10.1155/2021/6814215

de Farias, J. H. C., Reis, A. S., Araújo, M. A. R., Araújo, M. J. A. M., Assunção, A. K. M., de Farias, J. C., Fialho, E. M. S., Silva, L. A., Costa, G. C., Guerra, R. N. M., Ribeiro, M. N. S., & do Nascimento, F. R. F. (2014) Effects of Stingless Bee Propolis on Experimental Asthma. Evidence Based Complementary and Alternative Medicine, (2014). 1-8. https://doi.org/10.1155/2014/951478

Ekawati, R. A., Husnul, S. N., & Herawati, D. (2018). Identifikasi Kuman pada Pus dari Luka Infeksi Kulit. Jurnal Sains Health, 2(1). 31-35. https://doi.org/10.51804/jsh.v2i1.174.31-35

Francoy, T. M., Silva, R. A. O., & Nunes, S. P. (2019). Gen-der Identification of Five Genera of Stingless Bees (Apidae, Meliponini) Based on Wing morphology Genet. Genetics and Molecular Research, 8(1), 207-214. http://doi.org/10.4238/vol8-1gmr557

Grumezescu, A. M., & Holban, A. M. (2018). Therapeutic Probiotic Unconventional Food. Cambridge: Academic Press.

Handayani, F., Apriliana, A., & Natalia, H. (2019). Karakterisasi dan Skrining Fitokimia Simplisia Daun Selutui Puka (Tabernaemontana macracarpa Jack). Jurnal Ilmiah Ibnu Sina, 4(1), 49-58. https://doi.org/10.36387/jiis.v4i1.285

Hermalinda, R., Taufiqurrahman, I., & Helmi, Z. N. (2019). Total Flavonoid Content Analysis of Ramania Leaves Extract Using Ethanol, Methanol and N-Hexane as Solvents. Dentino : Journal Kedokteran Gigi, 4(1), 60-63.

Husain, D. R., Gunawan, S., & Sulfahri, S. (2020). Antimicrobial Potential of Lactic Acid Bacteria from Domestic Chickens (Gallus domesticus) from South Celebes, Indonesia, in Different Growth Phases : In Vitro Experiments Supported By Computational Docking. Iranian Journal of Microbiology, 12 (1), 62-69.

Ismarani, D., Pratiwi, L., & Kusharyanti, I. (2016). Formulasi Gel Pacar Air (Impatiens balsamina Lim.) terhadap Propionibacterium acnes dan Staphylococcus epidermidis. Pharmaceutical Sciences and Research, 1(1), 30-45. https://doi.org/10.7454/psr.v1i1.3504

Jamaluddin, A. W., Mursalim, M. F., & Apada, A. M. S. (2019). Combination of Propolis Extract (Trigona Sp) and Ginger Rhizome (Zingiber officinale Roscoe) as Alternative Antibiotics Against Escherichia coli In Vitro. Indonesian Journal of Pharmaceutical Science and Technology, 6(3), 112-117. https://doi.org/10.24198/ijpst.v6i3.20831

Jannah, H., & Safnowandi. (2018). Identifikasi Jenis Tumbuhan Obat di Kawasan Desa Batu Mekar Kecamatan Lingsar Kabupaten Lombok Barat. Bioscientist : Jurnal Ilmiah Biologi, 6(1), 1-15. https://doi.org/10.33394/bioscientist.v6i1.938

Lestari, F., & Andriani, S. (2020). Fitokimia Tumbuhan Berkhasiat Obat Tradisional di Kalimantan Selatan dan Kalimantan Tengah. Jurnal Galam, 1(2). 79-92. http://doi.org/10.20886/GLM.2021.1.2.79-92

Lopes, A. J. O., Vasconcelos, C. C., Pereira, F. A. N., Silva, R. H. M., Queiroz, P. F. d. S., Fernandes, C. V., Garcia, J. B. S., Ramos, R. M., da Rocha, C. Q., Lima, S. T. d. J. R. M., Cartágenes, M. d. S. d. S., & Ribeiro, M. N. d. S. (2019). Anti Inflammatory and Activity and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee Melipona fasciculata. International Journal of Moleculer Science, 20(4). 1-20. http://doi.org/10.3390/ijms20184512

Lutpiatina, L. (2015). Efektivitas Ekstrak Propolis Lebah Kelutut (Trigona spp) dalam Menghambat Pertumbuhan Salmonella typhi, Staphylococcus aureus, dan Candida albicans. Jurnal Skala Kesehatan, 6(1), 6-13. https://doi.org/10.31964/jsk.v6i1.32

Mardiah. (2017). Uji Resistensi Staphylococcus aureus terhadap Antibiotik, Amoxillin, Tetracyclin, dan Propolis. Jurnal Ilmu Alam dan Lingkungan, 8(2), 1-6. https://doi.org/10.20956/jal.v8i16.2978

Mayangsari, A. (2013). Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) Ekstrak Propolis Lawang terhadap Fusobacterium nucleatum. Skripsi. Fakultas Kedokteran Gigi Universitas Airlangga Surabaya.

Murtiwi, M. T. (2014). Aktivitas Antibakteri Ekstrak Etanol Daun (Macaranga tanarius (L.) Mull. Arg.) terhadap (Streptococcus pyogenes) ATCC 19615. Skripsi. Universitas Sanata Dharma Yogyakarta.

Nadhilla, N. F. (2014). The Activity of Antibacterial Agent of Honey Against Staphylococcus aureus. Medical Journal of Lampung University, 3(7), 94-101.

Nafisah, M., Tukiran., Suyatno., & Nurul, H. (2014) Uji Skrining Fitokimia pada Ekstrak Heksan, Kloroform, dan Metanol dari Tanaman Patikan Kebo (Euphorbia hirta). In Prosiding Seminar Nasional Kimia (pp. 279-286). Surabaya, Indonesia: Universitas Negeri Surabaya.

Ningsih, A. P., Nurmiati., & Agustien, A. (2013). Uji Aktivitas Antibakteri Ekstrak Kental Tanaman Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Biologi Universitas Andalas, 2(3), 207-213. https://doi.org/10.25077/jbioua.2.3.%25p.2013

Pratiwi, R. H. (2017). Mekanisme Pertahanan Bakteri Patogen terhadap Antibiotik. Jurnal Pro-Life, 4(3), 418-429. https://doi.org/10.33541/jpvol6Iss2pp102

Pujirahayu, N., Ritonga, H., & Uslinawaty, Z. (2014). Properties and Flavonoids Content in Propolis of Some Extraction Method of Raw Propolis. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 338-340.

Purwatiningsih, T. I., Yustina, Y. S., & Widodo. (2014). Aktivitas Senyawa Fenol dalam Buah Mengkudu (Morinda citrifolia) sebagai Antibakteri Alami untuk Bakteri penyebab Mastitis. Buletin Peternakan, 38(1), 59-64. https://doi.org/10.21059/buletinpeternak.v38i1.4618

Rismawati, S. N., & Ismiyati. (2017). Pengaruh Variasi PH terhadap Kadar Flavonoid pada Ekstraksi Propolis dan Karakteristiknya sebagai Antimikroba. Jurnal Konversi, 6(2), 89-94. https://doi.org/10.24853/konversi.6.2.89-94

Riyanto, E. F., Nurjanah, A. N., Ismi, S. N., & Suhartati, R. (2019). Daya Hambat Ekstrak Etanol Bunga Telang (Clitoria Ternatea L) terhadap Bakteri Perusak Pangan. Jurnal Kesehatan, 19(2), 218-225. http://doi.org/10.36465/jkbth.v19i2.500

Rocha, V. M., Portela, R. D., Anjos, J. P. D., Souza, C. O. D., & Guez, M. A. U. (2023). Stingless Bee Propolis : Composition, Biological Activities and its Applications in the Food Industry. Food Production, Processing and Nutrition, 5(1), 1-13. https://doi.org/10.1186/s43014-023-00146-z

Rosyidi, D., Radiati, E. L., Minarti, S., Mustakim, M., Susilo, A., Jaya, F., & Azis, A. (2018). Perbandingan Sifat Antioksidan Propolis pada Dua Jenis Lebah (Apis mellifera dan Trigona sp.) di Mojokerto dan Batu Jawa Timur Indonesia. Jurnal Ilmu dan Teknologi Hasil Ternak, 13(2), 108-117. https://doi.org/10.21776/ub.jitek.2018.013.02.5

Sapara, T. U., Waworuntu, O., & Juliatri. (2016). Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens balsamina L.) terhadap Pertumbuhan Porphyromonas gingivalis. Jurnal Ilmiah Pharmacon, 5(4), 10-17. https://doi.org/10.35799/pha.5.2016.13968

Saputera, M. M. A., Marpaung, T. W. A., & Ayuchecaria, N. (2019). Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis Hassk) terhadap Bakteri Escherichia coli melalui Metode Sumuran. Jurnal Ilmiah Manuntung : Sains, Farmasi, dan Kesehatan, 5(2), 167-173. https://doi.org/10.51352/jim.v5i2.267

Supomo., Sa'adah, H., Syamsul, E. S., Kintoko., Witasari, H. A., & Noorcahyati. (2021). Khasiat Tumbuhan Akar Kuning Berbasis Bukti. Makassar: Media Pustaka.

Syafrizal., Hariani, N., & Budiman. (2016). Analisis Fitokimia, Toksisitas dan Antioksidan Ekstrak Serbuk Sari (Bee pollen) Lebah Trigona spp. Proceeding of Mulawarman Pharmaceutical (pp. 408-416). Samarinda, Indonesia: Universitas Mulawarman.

Utami, E. R., & Rosa, Y. (2021). Aktivitas Antibakteri Ekstrak Etanol Daun Pandan Wangi (Pandanus amaryfolius) terhadap Staphylococcus aureus. Jurnal kesehatan : Jurnal Ilmiah Multi Sciences, 11(1), 61-71. https://doi.org/10.52395/jkjims.v11i01.324

Villanueva, X., Zhen, L., Ares, J. N., Vackier, T., Lange, H., Crestini, C., & Steenackers, H. P. (2023). Effect of Vhemical Modifications of Tannins on Their Antimicrobial and Antibiofilm Effect Against Gram-Negative and Gram-Positive Bacteria. Frontiers in Microbiology, 13(1), 1-15. https://doi.org/10.3389/fmicb.2022.987164

Walsh, T. R., Efthimiou, J., & Dréno, B. (2016). Systematic Review of Antibiotic Resistance in Acne an Increasing Topical and Oral Threat. The Lancet Infectious Diseases, 16(3). 23-33. https://doi.org/10.1016/S1473-3099(15)00527-7

Wardaniati, I., & Gusmawarni, V. (2021). Uji Aktivitas Antibakteri Ekstrak Etanol Propolis terhadap Streptococcus mutans. Jurnal Farmasi Higea, 13(2), 115-123. http://dx.doi.org/10.52689/higea.v13i2.372

Yuan, G., Guan, Y., Yi, H., Lai, S., Sun, Y., & Cao, S. (2021). Antibacterial Activity and Mechanism of Plant Flavonoids to Gram Positive Bacteria Predicted from Their Lipophilicities. Scientific Reports, 11(1), 1-15. http://doi.org/10.1038/s41598-021-90035-7

Yusop, S. A. T. W., Asaruddin, M. R., Sukairi, A. H., & Sabri, W. M. A. W. (2018). Cytotoxicity and Antimicrobial Activity of Propolis from Trogina itama Stingless Bees against Staphlococcus aureus and Escherichia coli. Indonesian Journal of Pharmaceutical, 1(1), 13-20. https://doi.org/10.24198/ijpst.v1i1.16118




DOI: https://doi.org/10.33394/bioscientist.v11i2.9373

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