Karakterisasi dan Uji Aktivitas Antimikroba Bakteri Kandidat Probiotik dari Terasi Udang Rebon

Herlinda Mawardika, Krisna Kharisma Pertiwi, Dwi Wahyuni, Qosania Wilda Aulia

Abstract


Probiotics are live microorganisms that are widely used to help maintain a healthy digestive system. Some compounds secreted by these bacteria have a bactericidal effect on pathogenic and putrefactive bacteria. Various types of probiotic bacteria can be obtained from fermented foods and drinks. This research aims to characterize bacteria from rebon shrimp paste in Macajah Village, Tanjung Bumi District, Bangkalan Regency, East Java Province which have potential as probiotics and test their antimicrobial activity against pathogenic bacteria. Bacteria were isolated using the Pour Plate technique using de Man Rogosa Sharpe Agar media. The bacterial isolates were then characterized by observing colony morphology, cells and biochemical properties. Next, antibacterial activity was tested using the well diffusion method. The results showed that the four bacteria obtained from the samples were gram positive, round and rod shaped, catalase negative, homofermentative, grew at a temperature of 37-45°C, survived at NaCl concentrations of 4 and 6.5%, low pH, and bile salts. According to the characterization results, three bacterial isolates belonged to the genus Enterococcus and one other isolate belonged to the genus Bacillus. Bacillus isolate is a potential probiotic candidate, because it has inhibitory activity against Escherichia coli and Staphylococcus aureus.

Keywords


Inhibitory Activity, Characterization, Probiotic, Shrimp Paste.

Full Text:

Full Paper

References


Ansumar. (2018). Isolasi dan Karakterisasi Bakteri Asam Laktat (BAL) Kandidat Probiotik dari Terasi Tradisional di Pekanbaru. Skripsi. Universitas Riau.

Bintsis, T. (2018). Lactic Acid Bacteria as Starter Cultures: An Update in Their Metabolism and Genetics. AIMS Microbiology, 4(4), 665-684. https://doi.org/10.3934/microbiol.2018.4.665

Chikindas, M. L., Weeks, R., Drider, D., Chistyakov, V. A., & Dicks, L. M. (2017). Functions and Emerging Applications of Bacteriocins. Current Opinion of Biotechnology, 49(1), 23-28. https://doi.org/10.1016/j.copbio.2017.07.011

Cremers, C. M., Knoefler, D., Vitvitsky, V., Banerjee, R., & Jakob, U. (2014). Bile Salts Act as Effective Protein-Unfolding Agents and Instigators of Disulfide Stress In Vivo. In Proceedings of the National Academy of Sciences (pp. 1610-1619). United State, America: Proceedings of the National Academy of Sciences.

Delost, M. D. (2022). Introducion to Diagnostic Microbiology for The Laboratory Sciences (2nd edition). Burlington: Jones and Bartlett Learning.

Dillon, V. M. (2014). Natural Anti-Microbial Systems. Preservative Effects During Storage. Encyclopedia of Food Microbiology, 2014(1), 941-947. https://doi.org/10.1016/B978-0-12-384730-0.00238-X

Ekwi, D. N., Fossi, B. T., Leopold, T. N., & Tabe, E. S. (2021). Isolation, Molecular Identification, and Phylogenetic Analysis of Bacteriocinogenic Lactic Acid Bacteria from Traditionally Fermented Foods from Four Major Cities in Cameroon Against Salmonella Enterica Pathogens. International Journal of Innovation Science Research Technology, 6(9), 819-829.

Giyatno, D. C., & Retnoningrum, E. (2020). Asam Laktat Penghasil Eksopolisakarida dari Buah Kersen (Muntingia calabura L.). Jurnal Sains Dasar, 9(2), 42-49. https://doi.org/10.21831/jsd.v9i2.34523

Goa, T, Beyene, G., Mekonnen, M., & Gorems, K. (2022). Isolation and Characterization of Lactic Acid Bacteria from Fermented Milk Produced in Jimma Town, Southwest Ethiopia, and Evaluation of their Antimicrobial Activity against Selected Pathogenic Bacteria. Hindawi : International Journal of Food Science, 2022(1), 1-15. https://doi.org/10.1155/2022/2076021

Hawali, B. (2019). Potensi Antibakterial Bakteri Asam Laktat dari Terasi terhadap Pertumbuhan Escherichia coli. Skripsi. Universitas Brawijaya.

Juliyarsi, I., Hartini, P., Yuherman., Djamaan, A., Arief., Purwanto, H., Aritonang, S. N., Hellyward, J., & Purwati, E. (2018). Characterization of Lactic Acid Bacteria and Determination of Antimicrobial Activity in Tempoyak from Padang Pariaman District, West Sumatra, Indonesia. Pakistan Journal of Nutrition, 17(10), 506-511. https://doi.org/10.3923/pjn.2018.506.511

Khairunnisa, M., Helmi, T. Z., Darmawi., Dewi, M., & Hamzah, A. (2018). Isolasi dan Identifikasi Staphylococcus aureus pada Ambing Kambing Peranakan Etawa (PE). Jurnal Ilmiah Mahasiswa Veteriner, 2(4), 538-545. https://doi.org/10.21157/jim%20vet..v2i4.9331

Kuebutornye, F. K. A., Lu, Y., Abarike, E. D., Wang, Z., Li Y., & Sakyi, M. E. (2020). In Vitro Assessment of The Probiotic Characteristics of Three Bacillus Species from the Gut of Nile tilapia, Oreochromis niloticus. Probiotics & Antimicrobial Proteins, 12(2), 412-424. https://doi.org/10.1007/s12602-019-09562-5

Kusmiatun, A., Ilham., Abrori, M., Sudiarsa, I. N., Nisa, A. C., Aras, A. K., Insani, L., Wahyu., Jatayu, D., Fikriyah, A., Kiswanto, A., Undu, M. C., & Utami, D. A. S. (2022). Aplikasi Probiotik Multispesies Komersial untuk Meningkatkan Kinerja Pertumbuhan Udang Vaname (Litopenaeus vannamei). Journal Perikanan, 12(4), 734-745. http://doi.org/10.29303/jp.v12i4.402

Lepecka, A., Szymanski, P., Rutkowska, S., Iwanowska, K., & Krajewska, D. K. (2021). The Influence of Environmental Conditions on the Antagonistic Activity of Lactic Acid Bacteria Isolated from Fermented Meat Products. Foods, 10(10), 1-12. https://doi.org/10.3390/foods10102267

Lutfiah, N. A. (2015). Isolasi dan Identifikasi Bakteri Asam Laktat pada Susu Kambing Saanen (Capra aegagrus H.). Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Mukamto., Ulfah, S., Mahalina, W., Syauqi, A., Istiqfaroh, L., & Trimulyono, G. (2015). Isolasi dan Karakterisasi Bacillus sp. Pelarut Fosfat dari Rhizosfer Tanaman Leguminosae. Sains & Matematika, 3(2), 62-68.

Romadhon., Rianingsih, L., & Anggo, A. D. (2018). Aktivitas Antibakteri dari Beberapa Tingkatan Mutu Terasi Udang Rebon. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(1), 68-76. https://doi.org/10.17844/jphpi.v21i1.21263

Saiz, N. V., Belguesmia, Y., Raspoet, R., Auclair, E., Gancel, F., Kempf, I., & Drider, E. (2019). Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives To Antibiotic Growth Promoters During Food Animal Production. Frontier Microbiology, 10(1), 1-17. https://doi.org/10.3389/fmicb.2019.00057

Sathiyaseelan, A., Saravanakumar, K., Han, K., Naveen, K. V., & Wang, M. H. (2022). Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods. International Journal of Molecular Sciences, 23(1), 1-16. . https://doi.org/10.3390/ijms231710062

Setiarto, R. H. B. (2021). Bioteknologi Bakteri Asam Laktat untuk Pengembangan Pangan Fungsional. Bogor: Guepedia.

Shobharani, P., Padmaja, R. J., & Halami, P. M. (2015). Diversity in the Antibacterial Potential of Probiotic Cultures Bacillus licheniformis MCC2514 and Bacillus licheniformis MCC2512. Research in Microbiology, 166(6), 546-554. https://doi.org/10.1016/j.resmic.2015.06.003

Suardana, I. W., Septiara, H. K. A., & Suarsana, I. N. (2017). Karakteristik Fisikokimia Bakteriosin Asal Bakteri Asam Laktat Enterococcus durans Hasil Isolasi Kolon Sapi Bali. Buletin Veteriner Udayana, 9(2), 209-215. https://doi.org/10.21531/bulvet.2017.9.2.209

Widodo. (2019). Bakteri Asam Laktat Strain Lokal. Yogyakarta: Gadjah Mada University Press.

Widyaningrum, M. E., Evawati, D., & Saputra E. (2022). Pemasaran Produk Terasi Nusantara. Banyumas: PT. Pena Persada Kerta Utama.

Winarno, F. G., & Winarno, W. (2017). Mikrobioma Usus bagi Kesehatan Tubuh : Peran Probiotik, Prebiotik, dan Parabiotik. Jakarta: Gramedia Pustaka Utama.




DOI: https://doi.org/10.33394/bioscientist.v11i2.8981

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Creative Commons License
Bioscientist : Jurnal Ilmiah Biologi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License

Editorial Address: Pemuda Street No. 59A, Catur Building Floor I, Mataram City, West Nusa Tenggara Province, Indonesia