Isolasi Bakteri Endofit Balakacida (Chromolaena odorata) Asal Banda Aceh dan Uji Aktivitas Antimikroba terhadap Bakteri Patogen Pasteurella multocida dan Bacillus subtilis

Wahyu Eka Sari, Darmawi Darmawi, Rumi Sahara Zamzami, Henni Vanda, Nurliana Nurliana, Etriwati Etriwati, Luthfia Amanda

Abstract


Endophytic bacteria are bacteria that live in plant tissue without causing negative effects. Endophytic bacteria can produce bioactive compounds that can be used as antibacterial, anti-fungal, antiviral, anti-cancer, anti-diabetic, antimalarial and anti-immunosuppressive properties. Endophytic bacteria are found in all higher plants, one of which is balakacida (Chromolaena odorata). This study aims to isolate the endophytic bacteria balakacida plant from Banda Aceh, to test the antimicrobial activity against the pathogenic bacteria Pasteurella multocida and Bacillus subtilis. The samples used were young leaves of balakacida plant with the method of isolation and purification of endophytic bacteria and in vitro tests.The results showed that endophytic bacteria could be isolated from balakacida plant and showed inhibitory activity against the pathogenic bacteria Pasteurella multocida and Bacillus subtilis. Thus, the endophytic bacteria from balakacida have bioactive compounds that have potential as antimicrobials.

Keywords


Endophytic Bacteria, Balakacida, Antimicrobial, Pasteurella multocida, Bacillus subtilis.

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References


Adeyemi, A.I., Vincent, O.I., and Olujenyo, O.M. (2018). Phytochemical Screening and Antifungal Activity of Chromolaena odorata Extracts Against Isolate of Phytophthora megakarya Using Agar-Well Diffusion Method. Asian Journal of Medical and Biological Research, 4(1), 7-13.

Afizar, dan Iin, P. (2017). Bakteri Endofit Asal Akar Kopi dan Potensinya Sebagai Agen Pengendali Penyakit Akar Putih Rigidoporus microporus. BIOLEUSER, 1(2), 54-62.

Brooks, G.F., Carroll, K., and Butel, J.S. (2013). Jawetz, Melnick, & Adelberg`s Medical Microbiology. Ed ke-26. Philadelphia: McGraw-Hill Company Inc.

Clinical and Laboratory Standards Institute. (2013). Performance Standars for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards Institute.

Hanphakphoom, S., Suchada, T., Piyaporn, W., Niwat K., and Sukhumaporn, K. (2016). Antimicrobial Activity of Chromolaena odorataextracts Against Bacterial Human Skin Infections. Modern Applied Science, 10(2), 159- 171.

Iyapparaj, P., Maruthiah, T., Ramasubburayan, R., Prakash, S., Kumar, C., Immanuel, G., and Palavesam, A. (2013). Optimization of Bacteriocin Production by Lactobacillus sp. MSU3IR Against Shrimp Bacterial Pathogens. Aquatic Biosystems, 9(12), 1-10.

Mamangkey, J., Suryanto, D., Munir, E., dan Lutfia, A. (2019). First Report of Plant Growth Promoting Endophytic Bacteria from Medicinal Invasive Plants (Chromolaena odorata). In International Conference on Biological Sciences and Biotechnology (pp. 1-10). Moscow, Russian: IOP Publishing.

Oktavia, N., dan Sri, P. (2018). Isolasi dan Uji Antagonisme Bakteri Endofit Tapak Dara (Catharanthus roseus, L.) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Berkala Bioteknologi, 1(1), 6-12.

Rau, C.H., Adithya, Y., dan Herny, E.I.S. (2018). Isolasi, Identifikasi secara Molukuler Menggunakan Gen 16S rRNA, Dan Uji Aktivitas Antibakteri Bakteri Simbion Endofit yang Diisolasi dari Alga Halimeda opuntia. PHARMACON Jurnal Ilmiah Farmasi, 7(2), 53-61.

Sirinthipaporn, A., and Jiraungkoorskul, W. (2017). Wound Healing Property Review of Siam Weed, Chromolaena odorata. Pharmacognosy Reviews, 21(11), 35-38.

Siripan, O., Thamchaipenet, A., dan Surat, W. (2018). Enhancement of the Efficiency of Cd Phytoextraction Using Bacterial Endophytes Isolated from Chromolaena odorata, a Cd Hyperaccumulator. International Journal of Phytoremediation, 20(11), 1096-1105.

Susilowati, D.N., Ginanjar, H., Yuniarti, E., Setyowati, M., dan Roostika, I. (2018). Karakterisasi Bakteri Endofit Tanaman Purwoceng sebagai Penghasil Senyawa Steroid dan Antipatogen. Jurnal Littri, 24(1), 1-10.




DOI: https://doi.org/10.33394/bioscientist.v11i1.8041

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