Potensi Tepung Biji Kecipir (Psophocarpus tetragonolobus) sebagai Bahan Alternatif Sumber Nitrogen dalam Media Mannitol Salt Agar (MSA) untuk Pertumbuhan Staphylococcus aureus

Urip Urip, Nanda Yuniza Pratiwi, Erlin Yustin Tatontos, Maruni Wiwin Diarti

Abstract


The purpose of this study was to determine the potential of winged bean (Psophocarpus tetragonolobus) seed powder as an alternative medium for Mannitol Salt Agar (MSA) against the growth of Staphylococcus aureus bacteria. This research method is true experiment with Completely Randomized Design (CRD). The experimental unit of this research was winged bean seed flour and Staphylococcus aureus. Winged bean seed flour used with 5 treatments, namely 2 gr, 4 gr, 6 gr, 8 gr, and 10 gr with each replication 5 times. The results showed that the standard MSA as a control showed an average of 3211 colonies, the average colony at a concentration of 2% was 372 colonies, the average colony on a 4% concentration plate was 419 colonies, the average colony on a 6% concentration plate was 544 colonies, the average colony on an 8% concentration plate were 622 colonies, and the average colony on a 10% concentration plate was 816 colonies. The conclusion of this study is winged bean seed powder has the potential as an alternative source of nitrogen in Mannitol Salt Agar (MSA) media for the growth of Staphylococcus aureus.

Keywords


Mannitol Salt Agar (MSA), Staphylococcus aureus, Winged Bean (Psophocarpus tetragonolobus).

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References


Anjani, A.D., Aulia, D.L.N., dan Suryanti. (2022). Metodologi Penelitian Kesehatan. Banyumas: CV. Pena Persada.

Dinges, M.M., Orwin, P.M., and Schlievert, P.M. (2000). Exotoxins of Staphylococcus aureus. Clinical Microbiology Reviews, 13(1), 16-34.

Diyantika, D., Mufida, D.C., dan Misnawi. (2017). Perubahan Morfologi Staphylococcus aureus Akibat Paparan Ekstrak Etanol Biji Kakao (Theobroma cacao) Secara In Vitro. Journal of Agromedicine and Medical Sciences, 3(1), 25-33.

Fitrianto, N., Samiyarsih, S., Rohma, A., dan Sasongko, N.D. (2021). Profil Mikromorfologi Kecipir (Psophocarpus tetragonolobus L.) DC) Mutan Akibat Iradiasi Sinar Gamma Cobalt-60. PLANTROPICA: Journal of Agricultural Science, 5(2), 95-106.

Julianti, F. (2022). Pengaruh Penambahan Tepung Biji Kecipir (Psophocarpus tetragonolobus) pada Pakan Terhadap Tingkat Kematangan Gonad Ikan Karper (Cyprinus carpio). Skripsi. Universitas Mataram.

Lam, J.C., and Stokes, W. (2023). The Golden Grapes of Wrath – Staphylococcus aureus Bacteremia: A Clinical Review. The American Journal of Medicine, 136(1), 19-26.

Nocera, F.P., Ferrara, G., Scandura, E., Ambrosio, M., Fiorito, F., and Martino, L.D. (2022). A Preliminary Study on Antimicrobial Susceptibility of Staphylococcus spp. And Enterococcus spp. Grown on Mannitol Salt Agar in European Wild Boar (Sus scrofa) Hunted in Campania Region—Italy. Animals, 12(1), 1-12.

Novitasari, T.M. (2019). Potensi Ikan Teri Jengki (Stolephorus indicus) sebagai Bahan Aternatif untuk Pertumbuhan Bakteri Staphylococcus aureus.

Novitasari, T.M., Rohmi, dan Inayati, N. (2019a). Potensi Ikan Teri Jengki sebagai Bahan Media Alternatif untuk Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Analis Medika Biosains, 6(1), 1-15.

______. (2019b). Potensi Ikan Teri Jengki (Stolephorus indicus) sebagai Bahan Media Alternatif untuk Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Analis Medika Biosains, 6(1), 1-15.

Nurhidayanti. (2022). Perbandingan Media Alternatif Kacang Kedelai dan Media Nutrient Agar terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Indobiosains, 4(2), 47-53.

Prasetyo, G., Warasi, D.M., dan Minarti, S. (2021). Pemanfaatan Bahan Lokal sebagai Pollen Subtitute terhadap Produktivitas Anakan dan Lebah Madu (Apis mellifera) pada Musim Paceklik. SEMNAS HPPM (pp. 85-91). Malang, Indonesia: Fakultas Peternakan Universitas Brawijaya.

Pristianingrum, S., Zainiati, B.L., Muttaqiin, Z., Puspita, F.D., dan Arman, R. (2021). Deteksi Metichilin Resistance Staphylococcus aureus (MRSA) pada Peralatan Medis yang Digunakan di Ruang Rawat Inap RSUD Provinsi NTB. Jurnal Analis Medika Biosains, 8(1), 7-12.

Putra, S.F., Fitri, R., dan Fadilah, M. (2022). Pembuatan Media Tumbuh Bakteri Berbasis Lokal Material. Prosiding Seminar Nasional Biologi (pp. 1043-1050). Padang, Indonesia: Universitas Negeri Padang.

Rezekikasari, dan Harianto, R. (2019). Modifikasi Media Alternatif dari Sayuran untuk Analisis Kuantitatif Pertumbuhan Mikroorganisme Asal Tanah Gambut Kalimantan Barat dengan Metode TPC. Jurnal Teknologi Perkebunan dan Pengelolaan Sumberdaya Lahan, 9(1), 1-8.

Suhartati, R., Sulistiani, dan Nuraini, A. (2018). Pemanfaatan Serbuk Kacang Kedelai (Glycine max) sebagai Bahan Pembuatan Media Manitol Salt Agar (MSA) untuk Pertumbuhan Bakteri Staphylococcus. Prosiding Seminar Nasional dan Diseminasi Penelitian Kesehatan (pp. 163-167). Tasikmalaya, Indonesia: STIKes Bakti Tunas Husada.

Thohari, N.M., Pestariati, dan Istanto, W. (2019). Pemanfaatan Tepung Kacang Hijau (Vigna radiata L.) sebagai Media Alternatif NA (Nutrient Agar) untuk Pertumbuhan Bakteri Escherichia coli. E-Journal Analis Kesehatan Sains, 8(2), 725-737.




DOI: https://doi.org/10.33394/bioscientist.v10i2.6138

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