Analisa Total Bakteri Salmonella spp. pada Produk Ikan Cakalang Asap yang Dijual pada Beberapa Pasar di Kota Ambon

Fiyogi Derandy Alfarego Tuhumury, Martha Kaihena, Cecelia Anna Seumahu

Abstract


Skipjack tuna (Katsuwonus pelamis) is one of the dominant capture fisheries products in Maluku. This is supported by the geographical location of the Maluku Islands which are surrounded by the sea. Bacteria are often found in traditional processed fishery products such as smoking fish. This study aims to determine the total bacteria Salmonella spp. and differences in the abundance of Salmonella spp. on smoked skipjack (Katsuwonus pelamis) products sold in several markets in Ambon City. This research is an experimental research. The research was carried out by taking samples with a total of 5 samples from the people's market location, and bacterial calculations were carried out using a dilution technique. Bacteria that have been obtained from the dilution results, then isolated using the quadrant scratch method technique on SSA media (Salmonella Shigela Agar). Bacterial biochemical tests, including: indole test, Methyl-Red test, Voges Proskauer test, citrate test, and Triple Sugar Iron Agar (TSIA) test, and gram staining were performed to confirm/identify the isolated bacteria. The results showed that smoked skipjack sold at Mardika Market was identified as having the total number of Salmonella spp. respectively 6700 CFU/ml and 8400 CFU/ml. The total number of Salmonella spp. on smoked skipjack at Mardika market is different from the results of the calculation of the total bacteria of smoked skipjack sold in modern markets and small hative markets, which did not identify any Salmonella spp bacteria contamination.


Keywords


Total Bacteria, Salmonella spp., Skipjack, Smoked Fish.

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References


Aini, F. (2018). Isolasi dan Identifikasi Shigella sp. Penyebab Diare pada Balita. Bio-Site, 04(1), 1-40.

Akerina, F.O. (2018). Cemaran Mikroba pada Ikan Tuna Asap di Beberapa Pasar Tradisional Tobelo, Halmahera Utara, Indonesia. Jurnal Akuakultur: Pesisir dan Pulau-pulau Kecil, 2(1), 17-21.

Antriana, N. (2014). Isolasi Bakteri Asal Saluran Pencernaan Rayap Pekerja Macrotermes spp. Saintifika, 16(1), 18-28.

Arifin, I.A. (2015). Deteksi Salmonella sp. pada Daging Sapi di Pasar Tradisional dan Pasar Modern di Kota Makassar. Skripsi. Universitas Hasanuddin.

BPOM. (2008). Informatorium Obat Nasional Indonesia. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia.

Buton, M. (2020). Analisis Bakteri Salmonella pada Daging Sapi yang Dijual di Pasar Mardika Kota Ambon dan Implikasi pada Mata Kuliah Mikrobiologi. Skripsi. Institut Agama Islam Negeri (IAIN) Ambon.

Fallo, G., dan Sine, Y. (2016). Isolasi dan Uji Biokimia Bakteri Selulolitik Asal Saluran Pencernaan Rayap Pekerja (Macrotermes spp.). BIO-EDU: Jurnal Pendidikan Biologi, 1(2), 27-29.

Hadi, J., dan Widawati, L. (2015). Analisis Sanitasi dan Cemaran Mikroorganisme Ikan Asap Lele di Bengkulu. Journal Agritepa, 2(1), 57-68.

Haryati, K. (2020). Pengujian Kualitas Mikrobiologi Ikan Ekor Kuning Asap dari Pasar Youtefa Papua. Pengolahan Hasil Perikanan Indonesia, 23(3), 486-494.

Indrawan, R.A., Siswoko, dan Putri, R.I. (2015). Pengaturan Suhu pada Proses Pengasapan Ikan Bandeng pada Oven Menggunakan Kontrol PID. Jurnal Elkolind, 2(1), 67-74.

Safitri, E., Hidayati, N.A., dan Hertati, R. (2019). Prevalensi Bakteri Salmonella pada Ayam Potong yang Dijual di Pasar Tradisional Pangkalpinang. Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi, 4(1), 25-30.

Sari, R., dan Apridamayanti, P. (2014). Cemaran Bakteri Eschericia coli dalam Beberapa Makanan Laut yang Beredar di Pasar Tradisional Kota Pontianak. Kartika: Jurnal Ilmiah Farmasi, 2(2), 14-19.

Sartika, D., Hidayati, S., dan Fitriani, H. (2019). Kajian Cemaran Bakteri Patogen pada Produk Olahan Ikan. Jurnal Penelitian Pertanian Terapan, 19(2), 108-114.

Susanti, Fusvita, A., dan Janhar, I.A. (2016). Identifikasi Salmonella sp. pada Ikan Asap di Pasar Tradisional Kota Kendari. BioWallacea: Jurnal Penelitian Biologi (Journal of Biological Research), 3(2), 467-473.

Witono, J.R., Miryanti, Y.I.P.A., dan Yuniarti, L. (2013). Studi Kinetika Dehidrasi Osmosis pada Ikan Teri dalam Larutan Biner dan Terner. Bandung: Universitas Katolik Parahyangan.

Yuliani, N.S., dan Oematan, A.B. (2013). Identifikasi Mikrobiologi (Staphylococcus dan Coliform) pada Susu dan Daging serta Olahannya di Kota Jogjakarta. Partner, 20(1), 20-29.

Zairiful, Sukaryana, Y., dan Maghfiroh, K. (2020). Kajian Cemaran Salmonella sp. pada Daging Ayam Broiler di Pasar Tradisional dan Modern Kota Bandar Lampung. Jurnal Peternakan Terapan (PETERPAN), 3(1), 1-4.




DOI: https://doi.org/10.33394/bioscientist.v10i2.5901

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