Pengaruh Jenis Pelarut pada Ekstraksi Jahe Merah (Zingiber officinale var. rubrum) terhadap Aktivitas Bakteri Penyebab Penyakit pada Hewan Ternak In Vitro

Abdul Zainal Muttaqin, Abun Abun, Endang Sujana

Abstract


The use of antibiotics and commercial drugs for handling livestock can have dangerous side effects, so it is necessary to use natural alternative medicines. Red ginger is a potential plant used as traditional medicine. The content of metabolite compounds in red ginger consisting of groups: phenols, terpenoids, essential oils, and flavonoids are reported to be able to inhibit several pathogenic bacteria that cause disease in animals and humans. The purpose of this study was to determine the effect of different types of solvents in the extraction process of red ginger on the activity of disease-causing bacteria in livestock in vitro. Extraction was carried out by maceration method using several solvents consisting of: distilled water, 96% ethanol ethyl acetate, and n-hexane. Antibacterial activity test using agar diffusion method. The parameter tested is the diameter of the inhibition created around the disc paper. The results showed that red ginger extract using ethyl acetate, 96% ethanol, and n-hexane had a diameter of inhibition zone against Staphylococcus aureus bacteria of 3.38 mm; 3.17mm; and 3.70mm. In contrast to Escherichia coli bacteria, all solvents used for red ginger extraction were not found to have an inhibition zone against these bacteria.

Keywords


Solvent, Extraction, Red Ginger, Antibacterial.

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References


Ajayi, A.O., and Omomagiowawi, I.E. (2014). Antimicrobial Activities and Phytochemical Analysis of Zingiber officinale (Ginger) on Staphylococcus aureus and Streptococcus species. Mitteilungen Klosterneuburg, 64(2014).

Akram, N., Chaudhary, A.H., Ahmed, S., Ghuman, M.A., Nawaz, G., and Hussain, S. (2013). Isolation of Bacteria from Mastitis Affected Bovine Milk and Their Antibiogram. European Journal of Veterinary Medicine, 2(1), 38-46.

Awanis, M.A., dan Mutmainnah, A.A. (2016). Uji Anti Bakteri Ekstrak Oleoresin Jahe Merah (Zingiber officinale var. rubrum) terhadap Bakteri Streptococcus pyogenes. Jurnal Ilmiah Kedokteran, 3(1), 33-41.

Chmit, M., Kanaan, H., Habib, J., Abbass, M., Mcheik, A., and Chokr, A. (2014). Antibacterial and Antibiofilm Activities of Polysaccharides, Essential Oil, and Fatty Oil Extracted from Laurus nobilis Growing in Lebanon. Asian Pacific Journal of Tropical Medicine, 7(S1), S546-S552.

Dianasari, D., Puspitasari, E., Ningsih, I.Y., Triatmoko, B., dan Nasititi, F.K. (2020). Potensi Ekstrak Etanol dan Fraksi-fraksinya dari Tiga Varietas Jahe sebagai Agen Antibakteri terhadap Staphylococcus aureus. Pharmacon: Jurnal Farmasi Indonesia, 17(1), 9-16.

Gopi, S., Varma, K., and Jude, S. (2016). Study on Temperature Dependent Conversion of Active Components of Ginger. International Journal of Pharmacognosy and Phytochemical Research, 6(1), 1344-1347.

Handrianto, P. (2016). Uji Antibakteri Ekstrak Jahe Merah (Zingiber officinale var. Rubrum) terhadap Staphylococcus aureus dan Escherichia coli. Journal of Research and Technologies, 2(1), 1-4.

Kaushik, S., Jangra, G., Kundu, V., Yadav, J.P., and Kaushik, S. (2020). Anti-Viral Activity of Zingiber officinale (Ginger) Ingredients Against the Chikungunya Virus. Virus Disease, 31(3), 270-276.

Kurniawati, A., Widodo, W., Artama, W.T., and Yusiati, L.M. (2018). Study of Local Herb Potency as Rumen Modifier: the Effect of Red Ginger (Zingiber officinale var. rubrum) on Parameters of Ruminal Fermentation in Vitro. IOP Conference Series: Earth and Environmental Science, 119(1), 1-8.

Mai-Prochnow, A., Clauson, M., Hong, J., and Murphy, A.B. (2016). Gram Positive and Gram Negative Bacteria Differ in Their Sensitivity to Cold Plasma. Scientific Reports, 6(38610), 1-11.

Meneses, N.G.T., Martins, S., Teixeira, J.A., and Mussatto, S.I. (2013). Influence of Extraction Solvents on the Recovery of Antioxidant Phenolic Compounds from Brewer’s Spent Grains. Separation and Purification Technology, 108, 152-158.

Mukhriani, T. (2014). Ekstraksi, Pemisahan Senyawa, dan Identifikasi Senyawa Aktif. Jurnal Kesehatan, VII(2), 361-367.

Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., and De Feo, V. (2013). Effect of Essential Oils on Pathogenic Bacteria. Pharmaceuticals, 6(12), 1451-1474.

Nur, Y., Cahyotomo, A., Nanda, dan Fistoro, N. (2020). Profil GC-MS Senyawa Metabolit Sekunder dari Jahe Merah (Zingiber officinale) dengan Metode Etil Asetat, Etanol, dan Destilasi. Jurnal Sains dan Kesehatan, 3(3), 198-204.

Pamungkas, Y.P., dan Dewi, M. (2013). Efek Antibakteri Perasan Jahe Merah (Zingiber Officinale var. rubrum) terhadap Bakteri Escherichia coli Secara In Vitro. Jurnal Farmasetis, 2(2), 46-51.

Pasaribu, T. (2019). Peluang Zat Bioaktif Tanaman sebagai Alternatif Imbuhan Pakan Antibiotik pada Ayam. Jurnal Penelitian dan Pengembangan Pertanian, 38(2), 96-106.

Purbaya, S., Aisyah, L.S., Jasmansyah, J., dan Arianti, W.E. (2018). Aktivitas Antibakteri Ekstrak Etil Asetat Jahe Merah (Zingiber officinale Roscoe var. sunti) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Kartika Kimia, 1(1), 29-34.

Purwanto, A., Fajriyati, A.N., dan Wahyuningtyas, D. (2014). Pengaruh Jenis Pelarut terhadap Rendemen dan Aktivitas Antioksidan dalam Ekstrak Minyak Bekatul Padi (Rice Bran Oil). Ekuilibrium, 13(1), 29-34.

Romero, J.C.L., Ríos, H.G., Borges, A., and Simões, M. (2015). Antibacterial Effects and Mode of Action of Selected Essential Oils Components Against Escherichia coli and Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine, 2015.

Sari, K.I.P., Periadnadi, dan Nasir, N. (2013). Uji Antimikroba Ekstrak Segar Jahe-jahean (Zingiberceae) terhadap Staphylococcus aureus, Escherichia coli dan Candida albicans. Jurnal Biologi Universitas Andalas, 2(1), 20-24.

Widiastuti, D., dan Pramestuti, N. (2018). Uji Antimikroba Ekstrak Jahe Merah (Zingiber officinale) terhadap Staphylococcus aureus. SEL: Jurnal Penelitian Kesehatan, 5(2), 43-49.

Xie, Y., Yang, W., Tang, F., Chen, X., and Ren, L. (2015). Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Current Medicinal Chemistry, 22(1), 132-149.

Zhuang, Q.Y., Wang, S.C., Li, J.P., Liu, D., Liu, S., Jiang, W.M., and Chen, J.M. (2014). A Clinical Survey of Common Avian Infectious Diseases in China. Avian Diseases, 58(2), 297-302.




DOI: https://doi.org/10.33394/bioscientist.v10i2.5823

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