Induksi Keragaman Genetik pada Tanaman Alocasia Menggunakan Mutagen Kimia Kolkisin

Fitri Damayanti, Zakiah Fithah A’ini

Abstract


Alocasia green velvet (neon taro) is one of the ornamental plants that plant lovers hunt. The increasing standard of living and interest in beauty and uniqueness, the demand for this plant has increased. This provides an opportunity for ornamental plant farmers to develop the potential of Alocasia. Genetic diversity in Alocasia is very low because generally these plants are propagated such as vegetative. Therefore, it is necessary to increase genetic diversity so that new types of Alocasia are produced with more attractive leaf shapes, sizes, and coloration. Efforts that can be applied are mutation induction using a chemical mutagen, namely colchicine. The plant material was used that the tuber of the Alocasia plant. The study was conducted using a randomized block design with two factors, the colchicine concentration (0, 10, 15, 20, 25, and 30 mgL-1) and the immersion time (1 and 2 days). The 30 mgL-1 colchicine treatment gave better growth: producing the highest number of leaves, the longest root length, the highest number of shoots and the percentage of sprouting tubers that reached 100%. Colchicine treatment of 10 mgL-1 with immersion time of 1 day resulted in a different leaf shape. Colchicine treatments of 10, 15, 20, and 25 mgL-1 produced plants with younger leaf coloration than the control.  As well as the 30 mgL-1 colchicine treatment produced vigorous plants with larger stem sizes.

Keywords


Alocasia, Colchicine, Genetic Diversity, Mutagenic.

Full Text:

Full Paper

References


Anitha, K., Jawaharlal, M., Joel, J., and Surendranath, R. (2017). Induction of Polyploids and Isolation of Ploidy Variants Through Stomatal Parameters in Bougainvillea (Bougainvillea spp.). Int. J. Agric. Sci. Res, 7(1), 451-458.

Asih, N.P.S., Warseno, T., dan Kurniawan, A. (2015). Studi Inventarisasi Araceae di Gunung Seraya (Lempuyang), Karangasem, Bali. In Seminar Nasional Masyarakat Biodiversitas Indonesia (pp. 521-527). Surakarta, Indonesia: Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Surakarta.

Damayanti, F., Roostika, I., dan Samsurianto. (2011). Induksi Keragaman Somaklonal Tanaman Kantong Semar (Nepenthes mirabilis) dengan Mutagen Kimia Kolkisin secara In Vitro. In Seminar Nasional IX Pendidikan Biologi (pp. 583-588). Surakarta, Indonesia: Fakultas Keguruan dan Ilmu Pendidikan, Universitas Negeri Surakarta.

Dar, T.H., Raina, S.N., and Goel, S. (2017). Cytogenetic and Molecular Evidences Revealing Genomic Changes After Autopolyploidization: A Case Study of Synthetic Autotetraploid Phlox drummondii Hook. Physiol. Mol. Biol. Plants, 23(3), 641-650.

Grouh, M.S.H., Meftahizade, H., Lotfi, N., Rahimi, V., and Baniasadi, B. (2011). Doubling The Chromosome Number of Salvia Hians Using Colchicine: Evaluation of Morphological Traits of Recovered Plants. J. Med. Plant. Res, 5(19), 4892-4898.

Hannweg, K., Sippel, A., and Bertling, I. (2013). A Simple and Effective Method for The Micropropagation and In Vitro Induction of Polyploidy and The Effect on Floral Characteristics of The South African Iris, Crocosmia Aurea. South African Journal of Botany, 88, 367-372.

Hassan, J., Miyajima, I., Ozaki, Y., Mizunoe, Y., Sakai, K., and Zaland, W. (2020). Tetraploid Induction by Colchicine Treatment and Crossing with A Diploid Reveals Less-Seeded Fruit Production in Pointed Gourd (Trichosanthes dioica roxb.). Plants, 9(3), 1-16.

He, M., Gao, W., Gao, Y., Liu, Y., Yang, X., and Jiao, H. (2016). Polyploidy Induced by Colchicine in Dendranthema Indicum Var. Aromaticum, A Scented Chrysanthemum. Eur. J. Hortic. Sci, 81(4), 219-226.

Hosseini, H., Chehrazi, M., Sorestani, M.M., and Ahmadi, D. (2013). Polyploidy and Comparison of Diploid and Autotetraploid Seedling of Madagascar Periwinkle (Catharanthus roseus cv. Alba). Int. Res. J. Basic Appl. Sci, 4(2), 402-406.

Kushwah, K.S., Verma, R.C., Patel, S., and Jain, N. (2018). Colchicine Induced Polyploidy in Chrysanthemum carinatum L. Journal of Phylogenetics & Evolutionary Biology, 6(01), 1-4.

Lertsutthichawan, A., Ruamrungsri, S., Duangkongsan, W., and Saetiew, K. (2017). Induced Mutation of Chrysanthemum by Colchicine. International Journal of Agricultural Technology, 13, 2325-2332.

Li, Z., and Ruter, J.M. (2017). Development and Evaluation of Diploid and Polyploid Hibiscus moscheutos. HortScience, 52(5), 676-681.

Manzoor, A., Ahmad, T., Bashir, M.A., Baig, M.M.Q., Quresh, A.A., Shah, M.K.N., and Hafiz, I.A. (2018). Induction and Identification of Colchicine Induced Polyploidy in Gladiolus grandiflorus “White Prosperity”. Folia Horticulturae, 30(2), 307-319.

Pirkoohi, M.H., Keyvanloo, M., and Hassanpur, M. (2011). Colchicine Induced Polyploidy in Mint by Seed Treatment. Int. J. Agric. Crop. Sci, 3(4), 102-104.

Ramesh, H.L., Murthy, V.N.Y., and Munirajappa. (2011). Colchicine Induced Morphological Variation in Mulberry Variety M 5. The Bioscan, 6(1), 115-118.

Rejeb, I.B., Pastor, V., Mauch-Mani, B. (2014). Plant Responses to Simultaneous Biotic and Abiotic Stress: Molecular Mechanisms. Plants (Basel), 3(4), 458-475.

Sutapa, G.N., dan Kasmawan, I.G.A. (2016). Efek Induksi Mutasi Radiasi Gamma 60Co pada Pertumbuhan Fisiologis Tanaman Tomat (Lycopersicon esculentum L.). Jurnal Keselamatan Radiasi dan Lingkungan, 1(2), 5-11.

Vichiato, M.R.M., Vichiato, M., Pasqual, M., Rodrigues, F.A., and Castro, D.M.D. (2014). Morphological Effects of Induced Polyploidy in Dendrobium nobile Lindl. (Orchidaceae). Crop. Breed. Appl. Biotechnol, 14(3), 154-159.




DOI: https://doi.org/10.33394/bjib.v9i1.3753

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Creative Commons License
Bioscientist : Jurnal Ilmiah Biologi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.