Effects of Ethanol Extract of Mikania micrantha Kunth Leaves on the Hematological Profile of Male Rats (Rattus norvegicus L.) Exposed to Cigarette Smoke
DOI:
https://doi.org/10.33394/bioscientist.v14i3.22299Keywords:
Mikania micrantha Kunth leaves, cigarette smoke, hematological profileAbstract
This study aimed to evaluate the effects of Mikania micrantha Kunth leaf extract on erythrocyte count, hemoglobin concentration, hematocrit, leukocyte count, and platelet count in male rats. A completely randomized design (CRD) was employed using 20 male rats randomly allocated into five experimental groups. The normal control group received no treatment for 30 days, whereas the positive control group was exposed to cigarette smoke from three cigarettes per day for 30 days. The treatment groups (P1, P2, and P3) were exposed to the same amount of cigarette smoke for 30 days and were administered Mikania micrantha Kunth leaf extract at doses of 100, 200, and 300 mg/kg body weight, respectively, for 23 days. The experimental procedures included phytochemical screening and measurement of hematological parameters, including erythrocyte count, hemoglobin concentration, hematocrit, leukocyte count, and platelet count, using a hematology analyzer. The data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan’s multiple range test. The results showed that administration of Mikania micrantha Kunth leaf extract significantly affected erythrocyte count, hemoglobin concentration, and hematocrit (p < 0.001), as well as leukocyte count (p < 0.001) and platelet count (p = 0.001). Among the tested doses, 300 mg/kg body weight produced hematological parameter values that most closely approximated those of the normal control group.
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