Effectiveness of a Combined Chromolaena odorata L. Leaf and Turmeric Rhizome Ointment on Incision Wound Length in Mice Based on Macroscopic Assessment
DOI:
https://doi.org/10.33394/bioscientist.v14i3.22254Keywords:
Kirinyuh leaf, turmeric rhizome, wound healing, miceAbstract
This study aimed to determine whether a combination of Kirinyuh leaf (Chromolaena odorata L.) and turmeric rhizome extracts affects incision wound length in mice based on macroscopic evaluation. This experimental study employed a randomized post-test-only control group design within a completely randomized design (CRD). A total of 25 male mice were divided into five treatment groups. Combination ointments containing Kirinyuh leaf and turmeric rhizome extracts at a 3:1 ratio were prepared at concentrations of 2.5%, 5%, and 10%. An incision measuring 1 mm in length was created on the dorsal region of each mouse. The wounds were treated topically twice daily with the extract ointment, and macroscopic observations were performed daily from day 0 to day 14. The results showed no significant differences among the groups on day 7. By day 14, most groups had achieved wound closure. The P2 group treated with the 2.5% combination ointment exhibited the lowest mean wound length (0.426 ± 0.073 mm), whereas the negative control group showed the highest mean wound length (1.296 ± 0.804 mm). These findings indicate that the combination of Kirinyuh leaf (Chromolaena odorata L.) and turmeric rhizome extracts may contribute to the healing of incision wounds in mice.
References
Abdul Latif, M., Mustafa, A., Keong, L. C., & Hamid, A. (2024). Chromolaena odorata layered-nitrile rubber polymer transdermal patch enhanced wound healing in vivo. PLOS ONE, 19(3), e0295381. https://doi.org/10.1371/journal.pone.0295381
Almadani, Y. H., Vorstenbosch, J., Davison, P. G., & Murphy, A. M. (2021). Wound healing: A comprehensive review. Seminars in Plastic Surgery, 35(3), 141–144.
Arifin, H., & Silvia, R. (2017). Efek antiinflamasi krim ekstrak etanol daun kirinyuh (Chromolaena odorata (L.) R. M. King & H. Rob.) secara topikal dan penentuan jumlah sel leukosit pada mencit putih jantan. Jurnal Farmasi Higea, 9.
Arisonya, Susanti, Wibisono, G., & Aditya, G. (2014). Efektivitas ekstrak kunyit (Curcuma domestica) terhadap jumlah sel makrofag dan diameter pada lesi ulkus trauma tikus. Jurnal B-Dent, 1(2), 118–125.
Benjamin, V. T., et al. (1987). Phytochemical and antibacterial studies on the essential oil of Eupatorium odoratum. Pharmaceutical Biology.
Gushiken, L. F. S., Beserra, F. P., Bastos, J. K., Jackson, C. J., & Pellizzon, C. H. (2021). Cutaneous wound healing: An update from physiopathology to current therapies. Life, 11(7), 665. https://doi.org/10.3390/life11070665
Harbone, J. B. (1987). Metode fitokimia: Penentuan cara modern menganalisa tumbuhan (K. Padmawinata, Trans.). ITB.
Herman, A. (2023). Herbal products and their active constituents for diabetic wound healing—Preclinical and clinical studies: A systematic review. Pharmaceutics, 15(1), 281. https://doi.org/10.3390/pharmaceutics15010281
Kumari, A., Raina, N., Wahi, A., Goh, K. W., Sharma, P., Nagpal, R., Jain, A., Ming, L. C., & Gupta, M. (2022). Wound-healing effects of curcumin and its nanoformulations: A comprehensive review. Pharmaceutics, 14(11), 2288. https://doi.org/10.3390/pharmaceutics14112288
Mohanty, C., & Sahoo, S. K. (2017). Curcumin and its topical formulations for wound healing applications. Drug Discovery Today, 22(10), 1582–1592. https://doi.org/10.1016/j.drudis.2017.07.001
Nurhajanah, Agussalim, L., Iman, S. Z., & Hajiriah, T. L. (2020). Analisis kandungan antiseptik daun kirinyuh (Chromolaena odorata) sebagai dasar pembuatan gel pada luka. Bioscientist: Jurnal Ilmiah Biologi, 8(2).
Olawale, F., Olofinsan, K. A., & Iwaloye, O. (2022). Biological activities of Chromolaena odorata: A mechanistic review. South African Journal of Botany, 144, 44–57. https://doi.org/10.1016/j.sajb.2021.09.001
Putry, B. O., Harfiani, E., & Tjang, Y. S. (2021). Systematic review: Efektivitas ekstrak daun kirinyuh (Chromolaena odorata) terhadap penyembuhan luka studi in vivo dan in vitro. In Seminar Nasional Riset Kedokteran (SENSORIK II).
Robinson, T. (1995). Kandungan organik tumbuhan tinggi (6th ed.). ITB.
Sagala, N. S. (2009). Pemanfaatan semak bunga putih (Chromolaena odorata) terhadap pertumbuhan dan IOFC dalam ransum burung puyuh (Coturnix coturnix japonica) umur 1 sampai 42 hari. Universitas Sumatera Utara.
Sirinthipaporn, A., & Jiraungkoorskul, W. (2017). Wound healing property review of Siam weed, Chromolaena odorata. Pharmacognosy Reviews, 11(21), 35–38. https://doi.org/10.4103/phrev.phrev_53_16
Tortora, G. J., & Derrickson, B. H. (2009). Principles of anatomy and physiology (12th ed.). Wiley.
Vital, P. G., & Rivera, W. L. (2009). Antimicrobial activity and cytotoxicity of Chromolaena odorata (L. f.) King and Robinson and Uncaria perrottetii (A. Rich.) Merr. extracts. Journal of Medicinal Plants Research, 3(7), 511–518.
Wientarsih, I., Winarsih, W., & Sutardi, N. (2012). Aktivitas penyembuhan luka oleh gel fraksi etil asetat rimpang kunyit pada mencit hiperglikemik. Jurnal Veteriner, 13(3), 251–256.
Winarsih, W., Wientarsih, I., & Handharyani, E. (2007). Kajian aktivitas ekstrak rimpang kunyit (Curcuma longa) dalam proses persembuhan luka pada mencit sebagai model penderita diabetes. IPB University Scientific Repository.
Yenti, R., Afrianti, R., & Afriani, L. (2011). Formulasi krim etanol daun kirinyuh (Eupatorium odoratum L.) untuk penyembuhan luka. Majalah Kesehatan Pharma Medika, 3(1), 227–230.
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