Effects of Salicylic Acid Concentration and Elicitation Duration on Total Flavonoid Content in Binahong (Anredera cordifolia (Ten.) Steenis) Callus Cultures
DOI:
https://doi.org/10.33394/bioscientist.v14i3.21469Keywords:
Elicitor, flavonoid, salicylic acid, callus culture, binahong (Anredera cordifolia)Abstract
This study aimed to determine the optimal elicitor concentration and elicitation duration for increasing the total flavonoid content of binahong (Anredera cordifolia) callus cultures. A two-factor completely randomized design (CRD) was employed, with salicylic acid concentration and elicitation duration as the experimental factors. Nine treatment combinations were tested with six replicates each, consisting of salicylic acid concentrations of 0, 20, and 40 ppm applied for 7, 14, and 21 days. The observed parameters included callus morphology (color and texture), callus biomass, and total flavonoid content determined using UV–Vis spectrophotometry. The results showed that the callus was predominantly compact in texture and green in color. Callus fresh weight differed significantly among treatments (p = 0.008), with the highest value recorded in treatment E2W3 (14 days, 40 ppm), at 0.9367 g. In contrast, callus dry weight did not differ significantly among treatments (p = 0.170). Two-way ANOVA further showed that elicitation duration (p = 0.374), salicylic acid concentration (p = 0.691), and their interaction (p = 0.330) had no significant effect (p > 0.05) on the total flavonoid content of binahong (Anredera cordifolia) callus cultures. Overall, the combination of salicylic acid concentration and elicitation duration significantly affected callus fresh weight but did not significantly influence callus dry weight or total flavonoid content.
References
Afiyah, N. N., Surya, M. I., Ismaini, L., Azizah, E., & Saputro, N. W. (2022). Inisiasi kalus secara in vitro dari daun Talinum paniculatum (Jacq.) Gaertn. Buletin Kebun Raya, 23(5), 121–130. https://doi.org/10.55981/bkr.2022.801
Alba, T. M., Pelegrin, C. M. G. D., & Sobottka, A. M. (2020). Ethnobotany, ecology, pharmacology, and chemistry of Anredera cordifolia (Basellaceae): A review. Rodriguésia, 71, 01042019.
Ali, B. (2021). Salicylic acid: An efficient elicitor of secondary metabolite production in plants. Biocatalysis and Agricultural Biotechnology, 31, 101884.
Anggraito, Y. U., Susanti, R., Iswari, R. S., Yuniastuti, A., Lisdiana, Habibah, N. A., & Bintari, S. H. (2018). Metabolit sekunder dari tanaman: Aplikasi dan produksi. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang.
Angin, Y., Purwaningrum, Y., Asbur, Y., Rahayu, M. S., & Nurhayati, N. (2019). Pemanfaatan kandungan metabolit sekunder yang dihasilkan tanaman pada cekaman biotik. Agriland: Jurnal Ilmu Pertanian, 7(1), 39–47.
Arieswari, N. N. N., Astarini, I. A., Astiti, N. P. A., & Pramana, J. (2018). In vitro callus induction of Shiraz grape (Vitis vinifera L.) using different medium and growth regulator combination. International Journal of Biosciences and Biotechnology, 6(1), 25–33. https://doi.org/10.24843/IJBB.2018.v06.i01.p03
Dalming, T. (2022). Penetapan kadar flavonoid total ekstrak metanol kulit buah alpukat (Persea americana Mill.) dengan metode spektrofotometri UV-Vis. Jurnal Farmasi Pelamonia, 2(2), 20–24.
Dewi, K. P., Nugroho, L. H., Sasongko, A. B., & Hidayati, L. (2023). Pengaruh konsentrasi sukrosa terhadap kadar piperin pada kalus cabe Jawa (Piper retrofractum Vahl.). Biota, 159–168.
Ekawati, Y., Anggraeni, A., & Prawestri, A. D. (2022). Induksi kalus sisik umbi Lilium longiflorum Thunb. oleh auksin dan sitokinin, serta respons pertumbuhannya secara in vitro. Agrosaintek, 6(2), 28–37. https://doi.org/10.33019/agrosainstek.v6i2.316
Fitria, V. A., Sakdiyah, A. K., Kurniati, V., Putra, D. O. Y., Putri, N. Z., & Pratiwi, P. D. (2025). Profil farmakologi binahong (Anredera cordifolia) sebagai kandidat modern medicine: Tinjauan sistematis. Sinteza.
Girsang, I. E., Restiani, R., & Prasetyaningsih, A. (2023). Induksi kalus eksplan daun porang (Amorphophallus muelleri Blume) menggunakan kombinasi air kelapa dan IAA (indole acetic acid). Sciscitatio, 4(2), 65–76.
Gorni, P. H., Brozulato, M. D. O., Lourencao, R. D. S., & Konrad, E. C. G. (2017). Increased biomass and salicylic acid elicitor activity in fennel (Foeniculum vulgare Miller). Brazilian Journal of Food Technology, 20, e2016172.
Gowthami. (2018). Role of elicitors in plant defense mechanism. Journal of Pharmacognosy and Phytochemistry.
Hajam, Y. A., Lone, R., & Kumar, R. (2023). Role of plant phenolics against reactive oxygen species (ROS)-induced oxidative stress and biochemical alterations. In Plant phenolics in abiotic stress management (pp. 125–147). Springer Nature Singapore.
Husain, F., Megawati, M., Safir, A., Renaldy, M., Kadir, R., Fatimah, M. A., Sabrina, A. I., Shabrina, P. A. N., & Lembang, M. A. M. (2023). Pembuatan elisitor biosaka sebagai salah satu inovasi dalam pengurangan penggunaan pupuk kimia. Jurnal Pengabdian Masyarakat Hasanuddin, 4(2), 82–91.
Julianti, R. F., Nurchayati, Y., & Setiari, N. (2021). Pengaruh konsentrasi sukrosa dalam medium MA terhadap kandungan flavonoid kalus tomat (Solanum lycopersicum syn. Lycopersicum esculentum). Metamorfosa, 8(1), 141–149. https://doi.org/10.24843/metamorfosa.2020.v08.i01.p015
Kadapi, M., Sunarso, C., Erizon, M. S., Maharani, N. D., Hakim, M. S., & Zahra, I. H. A. (2024). Teknologi kultur in vitro untuk meningkatkan produksi metabolit sekunder pada berbagai tanaman obat. Jurnal Agrosains dan Teknologi, 9(1), 18–29.
Karak. (2019). Biological activities of flavonoids: An overview. International Journal of Pharmaceutical Sciences and Research.
Khyahrii, A. S., & Shetty, S. (2025). Enhanced curculigoside and phenolic compounds elevate antioxidant activity in C. orchioides Gaertn in vitro under exogenous augmentation with elicitors salicylic acid (SA) and polyethylene glycol (PEG) 6000. Journal of Health and Allied Sciences NU, 15(1), 125–131.
Kousalya, L., Seethapathy, P., Pandita, D., Packiaraj, S., Venkatesh, S., Sankaralingam, S., & Elansary, H. O. (2025). Chitosan (CTS) induced secondary metabolite production in Canscora decussata Schult.—An endangered medicinal plant. Kuwait Journal of Science, 52(1), 100306.
Linardi, M., Restiani, R., & Aditiyarini, D. (2022). Pengaruh asam salisilat terhadap kandungan flavonoid pada kultur kalus ginseng Jawa (Talinum paniculatum [Jacq.] Gaertn.). EduMatSains: Jurnal Pendidikan, Matematika dan Sains, 6(2), 443–458.
Moreno-Escamilla, J. O., Jiménez-Hernández, F. E., Alvarez-Parrilla, E., de la Rosa, L. A., Martínez-Ruiz, N. D. R., González-Fernández, R., ... Rodrigo-García, J. (2020). Effect of elicitation on polyphenol and carotenoid metabolism in butterhead lettuce (Lactuca sativa var. capitata). ACS Omega, 5(20), 11535–11546.
Muna, J. F., & Asworo, R. Y. (2023). Analysis of Rhodamine B in lip cream using colorimetric method by digital imaging. Jurnal Wiyata: Penelitian Sains dan Kesehatan, 10(1), 25–34. https://doi.org/10.56710/wiyata.v10i1.594
Oktafiana, N., Ummayah, S., Ningtyas, W. N., & Sugiharto, B. (2022). Regenerasi kalus embriogenik sorgum (Sorghum bicolor) menggunakan kombinasi ZPT dan mikronutrien. Agriprima, 6(1), 54–61. https://doi.org/10.25047/agriprima.v4i2.375
Paek, K.-Y., & Murthy, H. N. (Eds.). (2024). Production of plant secondary metabolites using plant tissue culture and bioreactor culture techniques. MDPI. https://doi.org/10.3390/books978-3-7258-0810-6
Pramanik, J., Kumar, A., Rustagi, S., Katyal, M., Thakur, S., Bora, J., Malik, S., Trehan, A., Talukdar, N., & Slama, P. (2024). A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs. BIOCELL, 48(4), 541–557. https://doi.org/10.32604/biocell.2023.029031
Pradhan, J., Pramanik, K., Jaiswal, A., Kumari, G., Prasad, K., Jena, C., & Srivastava, A. K. (2024). Biosynthesis of secondary metabolites in aromatic and medicinal plants in response to abiotic stresses: A review. Journal of Experimental Biology and Agricultural Sciences, 12(3), 318–334. https://doi.org/10.18006/2024.12(3).318.334
Prashariska, K., Pitoyo, A., & Solichatun. (2021). Pengaruh indole-3-acetic acid (IAA) dan benzyl amino purine (BAP) terhadap induksi dan deteksi alkaloid kalus kamilen (Matricaria chamomilla L.). Innofarm, 23(2), 104–114.
Ramirez-Estrada, K., & Vidal-Limon, H. (2016). Elicitation, an effective strategy for the biotechnological production of high-value-added bioactive compounds in plant cell factories. Molecules, 21(2). https://doi.org/10.3390/molecules21020182
Raza, A., Salehi, H., Rahman, M. A., Zahid, Z., Madadkar Haghjou, M., Najafi Kakavand, S., Charagh, S., Osman, H. S., Albaqomi, M., Zhuang, Y., Siddique, K. H. M., & Zhuang, W. (2022). Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants. Frontiers in Plant Science, 13, 961872.
Restiani, R., Aditiyarini, D., & Eddijanto, I. (2022). Elisitasi flavonoid menggunakan kitosan pada kultur kalus ginseng Jawa (Talinum paniculatum Gaertn.). Sciscitatio: Journal of Biological Science, 3(2), 90–99.
Rismayanti, A. Y., & Nafi’ah, H. H. (2021). Modifikasi media pada induksi kalus kopi arabika (Coffea arabica L.) berbuah kuning. Agro Wiralodra.
Rosniawaty, S., Bahjatien, I. D., Soleh, M. A., Ariyanti, M., & Sudirja, R. (2024). Potensi metil jasmonat dan asam salisilat sebagai elisitor dalam mempengaruhi pertumbuhan bibit tanaman secang (Caesalpinia sappan L.). Agroland, 31(1), 55–62.
Selwal, N., Supriadi, K., Rahayu, F., Sukmadjaja, D., Khamidah, A., Budiaarto, K., Antarlina, S. S., Tripatmasari, M., & Wani, A. K. (2025). Elicitation strategies for enhanced secondary metabolite synthesis in plant cell cultures and its role in plant defense mechanism. Plant Gene, 41, 100485. https://doi.org/10.1016/j.plgene.2024.100485
Setiawati, T., Astuti, A. L., Nurzaman, M., & Ratningsih, N. (2021). Analisis pertumbuhan dan kandungan total flavonoid kultur kalus krisan (Chrysanthemum morifolium Ramat) dengan pemberian asam 2,4-diklorofenoksiasetat (2,4-D) dan air kelapa. Pro-Life, 8(1), 32–44.
Sharifi, Y., & Nematzadeh, G. A. (2019). Effect of salicylic acid on phenols and flavonoids content in callus culture of Iranian sodab (Ruta graveolens): A threatened medicinal plant of north of Iran. Tabari Biomedical Student Research Journal, 1(3), 32–36.
Silvina, F., Isnaini, I., & Ningsih, W. (2021). Induksi kalus daun binahong merah (Basella rubra L.) dengan pemberian 2,4-D dan kinetin. Jurnal Agro, 8(2), 274–286.
Singh, A. A., Ghosh, A., Agrawal, M., & Agrawal, S. B. (2023). Secondary metabolites responses of plants exposed to ozone: An update. Environmental Science and Pollution Research, 30(38), 88281–88312.
Solin, N. W. N. M., & Siregar, L. A. M. (2025). Optimasi metode sterilisasi eksplan daun gambir (Uncaria gambir [Hunter] Roxb.) asal Pakpak Bharat secara in vitro. In Prosiding Seminar Nasional Integrasi Pertanian dan Peternakan (Vol. 3, No. 1, pp. 58–68).
Tariq, H., Asif, S., Andleeb, A., Hano, C., & Abbasi, B. H. (2023). Flavonoid production: Current trends in plant metabolic engineering and de novo microbial production. Metabolites, 13(1), 124.
Wang, G., Ren, Y., Bai, X., Su, Y., & Han, J. (2022). Contributions of beneficial microorganisms in soil remediation and quality improvement of medicinal plants. Plants, 11(23), 3200.
Widiastiti, I. M., Afsari, N. W. I., & Duniaji, A. S. (2023). Efektivitas desinfektan larutan pemutih (Bayclin) dalam mencegah mikroba kontaminan pada inkubator di Laboratorium Mikrobiologi. Jurnal Rekayasa dan Manajemen Agroindustri, 11(2), 262–269.
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