Potential Effects of Gandaria (Bouea macrophylla Griff.) Fruit Ethanol Extract on IL-6 Gene Expression in the Brain of Carbon Tetrachloride (CCl₄)-Induced White Rats (Rattus norvegicus L.)
DOI:
https://doi.org/10.33394/bioscientist.v14i3.21417Keywords:
Oxidative stress, CCl₄, gandaria fruit ethanol extractAbstract
This study aimed to evaluate the effect of gandaria fruit ethanol extract on IL-6 expression in the brain tissue of CCl₄-induced rats. A completely randomized experimental design was employed using 20 male white rats (Rattus norvegicus L.), which were assigned to five groups: normal control (KN), positive control (K+), treatment group 1 (P1) receiving 100 mg/kgBW of gandaria fruit ethanol extract, treatment group 2 (P2) receiving 150 mg/kgBW, and treatment group 3 (P3) receiving 200 mg/kgBW. IL-6 gene expression in brain tissue was analyzed using reverse transcription polymerase chain reaction (RT-PCR), followed by band intensity and relative density analyses. The relative density analysis revealed that the greatest reduction in IL-6 gene expression occurred in the P1 treatment group administered 100 mg/kgBW of gandaria fruit ethanol extract, with a fold change value of 2.5. These findings suggest that gandaria fruit ethanol extract may attenuate the inflammatory response induced by CCl₄ exposure through the suppression of IL-6 expression, with the 100 mg/kgBW dose exhibiting the most pronounced reduction based on relative density analysis.
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