IAA (Indole-3-Acetic Acid) Production by ACC Deaminase-Producing Rhizobacteria Isolated from Peanut Rhizosphere
DOI:
https://doi.org/10.33394/bioscientist.v14i3.20039Keywords:
ACC deaminase, IAA production dynamics, peanut, PGPR, rhizobacteriaAbstract
This study aimed to evaluate the indole-3-acetic acid (IAA)-producing capacity of rhizobacterial isolates obtained from the rhizosphere of peanut (Arachis hypogaea L.) and previously identified as ACC deaminase-producing bacteria under in vitro conditions. IAA production was assessed qualitatively and quantitatively. Qualitative detection was performed using the colorimetric Salkowski reagent assay, whereas quantitative determination was conducted spectrophotometrically by comparing sample absorbance values with an IAA standard curve. The results showed that all six rhizobacterial isolates, together with Escherichia coli used as a reference strain, exhibited IAA-producing activity, although the estimated IAA concentrations varied among isolates and incubation periods. Among the tested rhizobacteria, isolate I9 exhibited the highest IAA concentration, reaching 34.75 ppm after three days of incubation, and showed an overall increasing production trend during the incubation period. These findings demonstrate that the ACC deaminase-positive rhizobacterial isolates examined in this study also possess IAA-producing capacity, although their production profiles differ among isolates. The combined potential for IAA production and ACC deaminase activity suggests that these isolates warrant further evaluation as plant growth-promoting rhizobacteria (PGPR) for improving plant growth and development.
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