Effects of Storage Duration of Mature and Young Coconut Water on the Growth of Dendrobium bigibbum

Authors

  • Sekar Ode Narendra Universitas PGRI Semarang
  • Fenny Roshayanti Universitas PGRI Semarang
  • Lussana Rossita Dewi Universitas PGRI Semarang

DOI:

https://doi.org/10.33394/bioscientist.v14i2.19951

Keywords:

Dendrobium bigibbum, mature coconut water, young coconut water, storage duration, vegetative growth

Abstract

This study evaluated the effects of coconut water type and storage duration on the vegetative growth of Dendrobium bigibbum. A completely randomized factorial design was employed, consisting of two coconut water types—mature coconut water and young coconut water—and three storage durations: 0, 1, and 2 days. The experiment included six treatment combinations and an untreated control, with three replicates per treatment. The observed growth parameters were plant height, number of leaves, leaf length, and number of shoots. Data were analyzed using two-way analysis of variance at a 5% significance level. No statistically significant differences were detected among treatments for plant height, number of leaves, leaf length, or number of shoots (p > 0.05). Nevertheless, descriptive observations showed that mature coconut water stored for 0 days (AKTL0) produced the highest mean plant height and leaf length, whereas mature coconut water stored for 1 day (AKTL1) produced the highest mean number of leaves and shoots. These findings indicate that, under the conditions of this study, coconut water type and short-term storage for up to 2 days did not produce statistically detectable effects on the vegetative growth of D. bigibbum. However, the numerically higher values observed in the AKTL0 and AKTL1 treatments suggest that fresh or briefly stored mature coconut water warrants further investigation using larger sample sizes, longer observation periods, standardized storage conditions, and laboratory analyses of coconut water quality. This result is consistent with previous evidence that the response of Dendrobium to coconut water depends on its nutrient composition, endogenous hormones, and physicochemical properties, which may change during storage.

References

Ahmad, A. (2023). Kandungan klorofil, karotenoid, dan flavonoid microgreens sawi hijau (Brassica juncea L.) yang ditanam pada berbagai media tanam dan konsentrasi air kelapa. https://repository.unhas.ac.id/id/eprint/29494/

Amalia, A. C., Mubarok, S., & Nuraini, A. (2022). Respons anggrek Dendrobium terhadap perbedaan naungan dan aplikasi zat pengatur tumbuh. Kultivasi, 21(2), 127–134. https://doi.org/10.24198/kultivasi.v21i2.35029

Aqidah, A., Dewanti, P., & Narulita Alfian, F. (2022). Pertumbuhan anggrek Dendrobium hibrida pada kultur cair dengan penambahan BA dan NAA. Journal of Agricultural Science, 20(1). http://jurnal.unmuhjember.ac.id/

Bhattacharjee, B., Hossain, M. E., Ayesha, M. N., & Islam, S. S. (2023). Morphological diversity analysis of some existing and endangered Dendrobium orchid species in Bangladesh. Journal of Bio-Science, 30(2), 47–57. https://doi.org/10.3329/jbs.v30i2.68911

Detudom, R., Deetae, P., Wei, H., Boran, H., Chen, S., Siriamornpun, S., & Prakitchaiwattana, C. (2023). Dynamic changes in physicochemical and microbiological qualities of coconut water during postharvest storage under different conditions. Horticulturae, 9(12), Article 1284. https://doi.org/10.3390/horticulturae9121284

Fritsche, Y., Vinícius, M., Pinheiro, M., & Pedro, M. (2022). Light quality and natural ventilation have different effects on protocorm development and plantlet growth stages of the in vitro propagation of Epidendrum fulgens (Orchidaceae). South African Journal of Botany, 146, 864–874.

Ge, L., Yong, J. W. H., Goh, N. K., Chia, L. S., Tan, S. N., & Ong, E. S. (2005). Identification of kinetin and kinetin riboside in coconut (Cocos nucifera L.) water using a combined approach of liquid chromatography–tandem mass spectrometry, high-performance liquid chromatography, and capillary electrophoresis. Journal of Chromatography B, 829, 26–34.

Hönig, M., Plíhalová, L., Husičková, A., Nisler, J., & Doležal, K. (2018). Role of cytokinins in senescence, antioxidant defence and photosynthesis. International Journal of Molecular Sciences, 19(12), Article 4045. https://doi.org/10.3390/ijms19124045

Hwang, J. E., Park, H. B., Jeon, D. Y., Park, H. J., Kim, S., Lee, C. W., Kim, Y. J., & Yoon, Y. J. (2024). Effect of different basal media and organic supplements on in vitro seedling development of the endangered orchid species Dendrobium moniliforme (L.) Swartz. Plants, 13(19), Article 2721. https://doi.org/10.3390/plants13192721

Inkiriwang, A. E. B., Mandang, J., & Runtunuwu, S. (2016). Substitusi media Murashige dan Skoog/MS dengan air kelapa dan pupuk daun majemuk pada pertumbuhan anggrek Dendrobium secara in vitro. Jurnal Bios Logos, 6(1). https://doi.org/10.35799/jbl.6.1.2016.16258

Irmayanti, A., Prasetiyo, P., & Rahayu, P. (2025). Effect of young coconut water concentration on the growth of Dendrobium orchid seedlings. Bioscientist: Jurnal Ilmiah Biologi, 13(1), 318. https://doi.org/10.33394/bioscientist.v13i1.15056

Karmila, R., Zakiah, Z., & Mukarlina. (2022). Acclimatization of black orchid plantlets (Coelogyne pandurata Lindl.) with moringa leaf extract (Moringa oleifera Lamk.) as a biostimulant. Jurnal Biologi Tropis, 22(3), 954–961. https://doi.org/10.29303/jbt.v22i3.3593

Lakshanthi, J. M. T., & Seran, T. H. (2019). Survival rate and growth performance of in vitro-raised plantlets of orchid (Dendrobium sp.) in different hardening substrates. 4(3), 1–9.

Limbad, M., Gutierrez-Maddox, N., Hamid, N., Kantono, K., Liu, T., & Young, T. (2023). Microbial and chemical changes during fermentation of coconut water kefir beverage. Applied Sciences, 13(12), Article 7257. https://doi.org/10.3390/app13127257

Ma, Z., Ge, L., Lee, A. S. Y., Yong, J. W. H., Tan, S. N., & Ong, E. S. (2008). Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta, 610, 274–281.

Nisa, N. A., Rahayu, T., & Jayanti, G. E. (2021). Peranan BAP dan air kelapa pada medium VW terhadap organogenesis Dendrobium sp. Metamorfosa: Journal of Biological Sciences, 8(2), 298. https://doi.org/10.24843/metamorfosa.2021.v08.i02.p14

Pokimica, N., Ćosić, T., Uzelac, B., Ninković, S., & Raspor, M. (2024). Dissecting the roles of the cytokinin signaling network: The case of de novo shoot apical meristem formation. Biomolecules, 14(3), Article 381. https://doi.org/10.3390/biom14030381

Putri Choirunnisa, J., Marselina Ade Putri, M., Skolandia Lau, M., Yurianti Sartika Unggut, Y., Jefri Suhardi, S., Pangka Program Studi Agronomi, B., & Pertanian dan Peternakan Universitas Katolik Indonesia Santu Paulus Ruteng, F. (2024). The effect of rice-washing water and coconut water on the growth of tomato plants (Lycopersium esculentum Mill.) in Manggarai Regency. Mediagro, 20(2).

Putri, N. K. I. S., Darmawati, I. A. P., & Astawa, I. N. G. (2023). Pengaruh lama penyimpanan benih dan konsentrasi rendaman air kelapa terhadap viabilitas benih padi (Oryza sativa L.). Agrotrop: Journal on Agriculture Science, 13(2), 289. https://doi.org/10.24843/ajoas.2023.v13.i02.p12

Ramadhan, J., & Abror, M. (2022). The effect of nutrient concentration and liquid organic fertilizer on pakcoy plant growth and yield using a wick hydroponic system. 2(2). https://doi.org/10.21070/pels.v2i2.1271

Samad, S., & Mahmud, S. A. (2025). Efek pupuk organik air kelapa pada pertumbuhan dan hasil selada di Bigaro, Halmahera Timur. Journal of Comprehensive Science, 4(1). https://doi.org/10.59188/jcs.v4i1.2958

Santosa, F. A., Setiari, N., & Nurchayati, Y. (2025). Effect of different coconut water concentrations on the growth of Dendrobium spectabile (Blume) Miq. plantlets on MS medium. Jurnal Ilmu Pertanian Indonesia, 30(3), 533–539. https://doi.org/10.18343/jipi.30.3.533

Tungga, W., Tilaar, S. M. T., Tulung, J., Pongoh, J., Mamarimbing, R., & Sumayku, B. R. A. (2023). The effect of the plant growth regulator kinetin and coconut water on the growth and development of Dendrobium mirbelianum orchids in vitro. https://doi.org/10.35791/jat.v4i2.51921

Yao, J., Ke, X., Gu, X., Jiang, Z., Ying, Z., Lu, C., & Sun, C. (2026). Optimized substrate selection for enhanced orchid growth based on high-throughput lysimetric arrays. 27(5), 437–449. https://doi.org/10.1631/jzus.B2500195

Yong, J. W. H., Ge, L., Ng, Y. F., & Tan, S. N. (2009). The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules, 14(12), 5144–5164. https://doi.org/10.3390/molecules14125144

Yuliana, M. (2021). The effect of local microorganisms (MOL) as liquid organic fertilizer on the growth of Ipomoea reptans Poir. Jurnal Biota, 7(1). http://jurnal.radenfatah.ac.id/index.php/biota

Zakiyah, A., Setiari, N., & Nurchayati, Y. (2025). The growth of Dendrobium spectabile (Blume) Miq. orchid plantlets in Vacin and Went medium with different concentrations of coconut water. Berita Biologi, 24(2). https://doi.org/10.55981/berita_biologi.2025.222

Downloads

Published

2026-06-30

How to Cite

Narendra, S. O., Roshayanti, F., & Dewi, L. R. (2026). Effects of Storage Duration of Mature and Young Coconut Water on the Growth of Dendrobium bigibbum. Bioscientist : Jurnal Ilmiah Biologi, 14(2), 1119–1132. https://doi.org/10.33394/bioscientist.v14i2.19951

Issue

Section

Articles