Literature Review on the Potential of Pleurotus spp. Fungal Mycelium as a Biodegradable Packaging Material for Styrofoam Replacement
DOI:
https://doi.org/10.33394/bioscientist.v14i2.19818Keywords:
Fungal mycelium, biodegradable packaging, Pleurotus ostreatus, mycelium-based foam, food safetyAbstract
This literature review aimed to: (1) identify edible fungal species with potential applications as mycelium-based packaging materials; (2) compare mycelial characteristics in terms of substrate compatibility, mechanical properties, thermal stability, water absorption, and food safety; and (3) recommend the most suitable species for the development of biodegradable food packaging. A systematic scoping review was conducted following the framework of Arksey & O’Malley (2005) and PRISMA-ScR guidelines. Literature searches were performed in Scopus, Web of Science, ScienceDirect, and Google Scholar for publications from 2015 to 2024 using the keywords mycelium composite, mycelium-based foam, Pleurotus packaging, and biodegradable packaging fungi. Of the 312 articles initially identified, 47 met the inclusion criteria, namely empirical studies that assessed at least two material parameters and were published in English or Indonesian. The findings indicate that Pleurotus ostreatus exhibits several advantages, including rapid colonization time (7–14 days), suitable mycelium density (25–75 kg/m³), tensile strength (0.18–0.43 MPa), low thermal conductivity (0.03–0.07 W/m·K), and a high biodegradation rate in soil (60–90% per week). Pleurotus citrinipileatus demonstrated slightly lower mechanical performance but greater adaptability to mixed substrates. Both species are classified as generally recognized as safe (GRAS) by the FDA and do not produce significant levels of mycotoxins under standard cultivation conditions. Overall, P. ostreatus shows strong potential as a biodegradable packaging material to replace expanded polystyrene (EPS). However, industrial-scale experimental validation, food contact migration testing, and production standardization remain essential before commercial application.
References
Abhijith, R., Ashok, A., & Rejeesh, C. R. (2018). Sustainable packaging applications from mycelium to substitute polystyrene: A review. Materials Today: Proceedings, 5(1), 2139–2139. https://doi.org/10.1016/j.matpr.2017.09.211
Anwar, K., Setyawati, R. O., & Karisma, C. K. (2014). Inventarisasi dan karakterisasi jamur liar yang dapat dikonsumsi di Desa Wonojati Kecamatan Gondangwetan Kabupaten Pasuruan–Jawa Timur. Proceeding Biology Education Conference: Biology, Science, Environmental, and Learning, 11(1). https://jurnal.uns.ac.id/prosbi/article/view/7732
Appels, F. V. W., Camere, S., Montalti, M., Karana, E., Jansen, K. M. B., Dijksterhuis, J., Krijgsheld, P., & Wösten, H. A. B. (2019). Fabrication factors influencing mechanical, moisture- and water-related properties of mycelium-based composites. Materials & Design, 161, 64–71. https://doi.org/10.1016/j.matdes.2018.11.027
Arksey, H., & O’Malley, L. (2005). Scoping studies: Towards a methodological framework. International Journal of Social Research Methodology, 8(1), 19–32. https://doi.org/10.1080/1364557032000119616
Attias, N., Reid, M., Mijowska, S. C., Dobryden, I., Isaksson, M., Pokroy, B., Abitbol, T., & Grobman, Y. J. (2020). Bioinspired structural color from natural waste material. Science of Advanced Materials, 5(1), 1–12.
Barnes, D. K. A., Galgani, F., Thompson, R. C., & Barlaz, M. (2009). Accumulation and fragmentation of plastic debris in global environments. Philosophical Transactions of the Royal Society B, 364(1526), 1985–1998. https://doi.org/10.1098/rstb.2008.0205
Bellettini, M. B., Fiorda, F. A., Maieves, H. A., Teixeira, G. L., Ávila, S., Hornung, P. S., Maccari Junior, A., & Ribani, R. H. (2019). Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26(4), 633–646. https://doi.org/10.1016/j.sjbs.2016.12.005
Cerimi, K., Akkaya, K. C., Pohl, C., Schmidt, B., & Neubauer, P. (2019). Fungi as source for new bio-based materials: A patent review. Fungal Biology and Biotechnology, 6(1). https://doi.org/10.1186/s40694-019-0080-y
Editor. (2020). Replaces Styrofoam with mushroom bioplastics. Bioplastics News. Retrieved July 28, 2025, from https://bioplasticsnews.com/2020/02/07/ikea-styrofoam-mushroom-bioplastics/
Elsacker, E., Vandelook, S., Van Wylick, A., Ruytinx, J., De Laet, L., & Peeters, E. (2020). A comprehensive framework for the production of mycelium-based lignocellulosic composites. Science of the Total Environment, 725, 138334. https://doi.org/10.1016/j.scitotenv.2020.138334
European Food Safety Authority. (2020). Guidance on the risk assessment of substances present in food intended for infants below 16 weeks of age. EFSA Journal, 18(4), Article e06053. https://doi.org/10.2903/j.efsa.2020.6053
Fitidarini, N. L., & Damanhuri, E. (2011). Timbulan sampah styrofoam di Kota Bandung [Styrofoam waste generation in the city of Bandung]. Jurnal Teknik Lingkungan, 17. http://www.bandung.go.id
Genualdi, S., Nyman, P., & Begley, T. (2014). Updating the migration of styrene monomer and oligomers from polystyrene food contact materials to foods and food simulants. Food Additives & Contaminants: Part A, 31(4), 723–733. https://doi.org/10.1080/19440049.2014.888780
Geyer, R., Jambeck, J. R., & Law, K. L. (2017). Production, use, and fate of all plastics ever made. Science Advances, 3(7), Article e1700782. https://doi.org/10.1126/sciadv.1700782
Gunde-Cimerman, N., & Cimerman, A. (1995). Pleurotus fruiting bodies contain the inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase–lovastatin. Experimental Mycology, 19(1), 1–6. https://doi.org/10.1006/emyc.1995.1001
Haneef, M., Ceseracciu, L., Canale, C., Bayer, I. S., Heredia-Guerrero, J. A., & Athanassiou, A. (2017). Advanced materials from fungal mycelium: Fabrication and tuning of physical properties. Scientific Reports, 7, Article 41292. https://doi.org/10.1038/srep41292
Hasbi, A., Hamsiati, & Suni, N. S. (2024). Kemasan hijau di industri makanan dan minuman dalam mendukung pariwisata berkelanjutan. CV. Mega Press.
International Agency for Research on Cancer. (2002). IARC monographs on the evaluation of carcinogenic risks to humans: Vol. 82. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene.
Jones, M., Mautner, A., Luenco, S., Bismarck, A., & John, S. (2020). Engineered mycelium composite structures from fungal biorefineries: A critical review. Materials & Design, 187, Article 108397. https://doi.org/10.1016/j.matdes.2019.108397
Kostić, M., Milovanović, I., Petrović, J., Stojanović, M., & Radulović, N. (2024). Safety assessment of edible mushrooms: Absence of regulated mycotoxins in Pleurotus ostreatus. Comprehensive Reviews in Food Science and Food Safety, 23(2), 1458–1476. https://doi.org/10.1111/1541-4337.13245
Krauter, V., Bauer, A.-S., Milousi, M., Dörnyei, K. R., Ganczewski, G., Leppik, K., Krepil, J., & Varzakas, T. (2022). Cereal and confectionary packaging: Assessment of sustainability and environmental impact with a special focus on greenhouse gas emissions. Foods, 11(9), Article 1347. https://doi.org/10.3390/foods11091347
Kumar, K., Mehra, R., Guiné, R. P. F., Lima, M. J., Kumar, N., Kaushik, R., Ahmed, N., Yadav, A. N., & Kumar, H. (2021). Edible mushrooms: A comprehensive review on bioactive compounds with health benefits and processing aspects. Foods, 10(12), Article 2996. https://doi.org/10.3390/foods1012296
Lacourt, C., Mukherjee, K., Garthoff, J., O’Sullivan, A., Meunier, L., & Fattori, V. (2024). Recent and emerging food packaging alternatives: Chemical safety risks, current regulations, and analytical challenges. Comprehensive Reviews in Food Science and Food Safety, 23(6), 1–29. https://doi.org/10.1111/1541-4337.70059
Lelivelt, R. J. J., Lindner, G., Teuffel, P., & Lamers, H. (2015). The production process and compressive strength of mycelium-based composites made from cotton waste. In Proceedings of the First International Conference on Bio-Based Building Materials. Clermont-Ferrand, France.
Levac, D., Colquhoun, H., & O’Brien, K. K. (2010). Scoping studies: Advancing the methodology. Implementation Science, 5(1), Article 69. https://doi.org/10.1186/1748-5908-5-69
Majib, N. M., Sam, S. T., Yaacob, N. D., Rohaizad, N. M., & Tan, W. K. (2023). Characterization of fungal foams from edible mushrooms using different agricultural wastes as substrates for packaging material. Polymers, 15(4). https://doi.org/10.3390/polym15040873
Moniruzzaman, M., Tuong, T. T., et al. (2025). A review on pharmacological insights of edible and medicinal mushroom-based β-glucans. Applied Biological Chemistry, 68, Article 41. https://doi.org/10.1186/s13765-025-01006-9
Panévska, A., Skočaj, M., & Križaj, I. (2021). Aegerolysins from mushrooms: Structure, function, and lipid interactions. Biochimica et Biophysica Acta (BBA) – Biomembranes, 1863(1), Article 183492. https://doi.org/10.1016/j.bbamem.2020.183492
Patricia, E. (2021). Green packaging: Mushroom-based packaging for sustainable development in Nigeria: A descriptive perspective. International Journal of Management Science and Entrepreneurship, 21(7).
Pohan, J. N., Kusumawati, Y. A., & Radhitanti, A. (2023). Mushroom mycelium-based biodegradable packaging material: A promising sustainable solution for food industry. E3S Web of Conferences, 426, Article 02128. https://doi.org/10.1051/e3sconf/202342602128
Prayudanti, A. A., & Sucipto, S. (2021). Halal and safety traceability of material, production, and serving of local food in Surabaya: A review. IOP Conference Series: Earth and Environmental Science, 924(1). https://doi.org/10.1088/1755-1315/924/1/012002
Pusat Perpustakaan dan Penyebaran Teknologi Pertanian, Kementerian Pertanian Republik Indonesia. (2018). Kiat budi daya jamur tiram (Cetakan 1). Pusat Perpustakaan dan Penyebaran Teknologi Pertanian. https://repository.pertanian.go.id/handle/123456789/5453
Puspa, A. (2022). Indonesia darurat sampah styrofoam. Media Indonesia. Retrieved July 25, 2025, from https://mediaindonesia.com/humaniora/539805/indonesia-darurat-sampah-styrofoam#goog_rewarded
Putri, H. C., Victor, J., Sugiarto, A., Kurniawan, M. F., Ayyub, D., & Sukmaraga, A. (2024). Pemanfaatan miselium pada jamur sebagai material biodegradable dalam desain kemasan berkelanjutan. Prosiding SENAM 2024: Desain Komunikasi Visual, 4, 1–12.
Rabelo, L. S., Tanaka, F. C., dos Santos, S. S., Aouada, F. A., & de Moura, M. R. (2023). The future of sustainable packaging: Exploring biodegradable solutions through extrusion, thermo-expansion, 3D printing and supercritical fluid from agro-industry waste. Polymers, 15(15), Article 3324. https://doi.org/10.3390/polym15153324
Ritchie, H., & Roser, M. (2018). Plastic pollution. Our World in Data. Retrieved July 28, 2025, from https://ourworldindata.org/plastic-pollution?utm_source=newsletter
Sankhla, I. S., Sharma, G., & Tak, A. (2020). Fungal degradation of bioplastics: An overview. In New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Advances in Application of Fungi and Fungal Metabolites: Environmental and Industrial Aspects. Elsevier. https://doi.org/10.1016/B978-0-12-821007-9.00004-8
Sharma, E., Bairwa, R., Lal, P., & Pattanayak, S. (2024). Edible mushrooms trending in food: Nutrigenomics, bibliometric perspectives and valuable applications. Heliyon, 10, Article e36963. https://doi.org/10.1016/j.heliyon.2024.e36963
Singh, A. (2025). Mushrooms as nutritional powerhouses: A review of their bioactive compounds and health benefits. Foods, 14(5), Article 741. https://doi.org/10.3390/foods14050741
SIPSN. (2025). Komposisi sampah. Retrieved July 25, 2025, from https://sipsn.kemenlh.go.id/sipsn/public/data/komposisi
Stamets, P. (2005). Mycelium running: How mushrooms can help save the world. Ten Speed Press.
Tricco, A. C., Lillie, E., Zarin, W., O’Brien, K. K., Colquhoun, H., Levac, D., Moher, D., Peters, M. D. J., Horsley, T., Weeks, L., Hempel, S., Akl, E. A., Chang, C., McGowan, J., Stewart, L., Hartling, L., Aldcroft, A., Wilson, M. G., Garritty, C., et al. (2018). PRISMA extension for scoping reviews (PRISMA-ScR). Annals of Internal Medicine, 169(7), 467–473. https://doi.org/10.7326/M18-0850
Yang, Z., Zhang, F., Still, B., White, M., & Bhowmik, P. (2021). Physical and mechanical properties of fungal mycelium-based biofoam. Journal of Materials in Civil Engineering, 33(1), Article 04020391.
Žužek, M. C., Barlič, A., Drašlar, K., Tomažič, S., Podobnik, M., Anderluh, G., & Sepčić, K. (2006). Toxic and lethal effects of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, in rodents. Toxicon, 48(3), 264–271. https://doi.org/10.1016/j.toxicon.2006.05.011
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