Pengaruh Ekstrak Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kadar Asam Urat Pada Tikus Putih (Rattus norvegicus) Yang Diinduksi LDL

Ifat Fariza, Achmad Ramadhan, Sutrisnawati Sutrisnawati, Hayyatun Mawaddah, Abd Rauf, Manap Trianto

Abstract


This study aims to determine the effect of Rosella flower extract (Hibiscus sabdariffa L.) on uric acid levels in White Rats (Rattus norvegicus) induced by LDL. The research method used is the Laboratory experiment method with a Completely Randomized Design (CRD) pattern. The number of samples used was 21 White Rats consisting of 7 treatment groups and 3 replications. The normal control group (KN) is a group that is only given food and drink, the negative control group (K-) is given LDL induction for 30 days, the positive control group (K+) is given LDL induction for 30 days and allopurinol, and the group given LDL induction for 30 days and Rosella flower extract, namely concentrations of 25% (PI), 50% (PII), 75% (PIII), and 100% (PIV) for 14 days. The data obtained were tabulated and analyzed using one-way ANOVA statistical test using SPSS version-30. The results showed that Rosella flower extract had a significant effect (p <0.05) on reducing uric acid levels. The effective concentration for reducing uric acid levels is a concentration of 50% (PII) compared to concentrations of 75% and 100% because there are chemical compounds that are antagonistic to the active compound, thus reducing the extract's ability to reduce blood uric acid levels. The results of the Rosella flower study can reduce uric acid levels so that it can be used as an alternative natural treatment.

Keywords


Rosella (Hibiscus sabdariffa L.); asam urat; tikus putih (Rattus norvegicus)

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References


Abou-Arab, A. A., Abou-Arab, E. A., & Abu-Salem, M. F. (2011). Physico-Chemical Assessment of Natural Sweeteners Steviosides Produced From Stevia rebaudiana Bertoni plant. African Journal of Food Science, 5(5), 281–286.

Apriani, A. A., Prabowo, W. C., & Ibrahim, A. (2016, April). Efek Antihiperurisemia Ekstrak Etanol Daun Mahkota Dewa (Phaleria macrocarpa) Scheff. Boerl.) pada Mencit Putih (Mus musculus). In Proceeding of Mulawarman Pharmaceuticals Conferences (pp. 96–103). Samarinda, Indonesia: Fakultas Farmasi, Universitas Mulawarman.

Dinayanti, T. (2010). Pengaruh Pemberian Seduhan Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kadar Kolesterol Total Serum Tikus Sprague-Dawley hiperkolesterolemik. Skripsi. Universitas Diponegoro, Semarang.

Haidar, Z. (2016). Si Cantik Rosella: Bunga Cantik Berjuta Khasiat. Jakarta: Edumania.

Idris, S., Lestari, R., & Lesmana, R. (2021). Pengaruh Pemberian Ekstrak Bunga Rosela (Hibiscus sabdariffa L.) Terhadap Kadar Asam Urat pada Penderita Hiperuresemia. Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, 8(1), 1–9.

Muhtadi, Idi, R., & Nurcahyanti, W. (2012). Penghambatan Ksantin Oksidase Oleh Kombinasi Ekstrak Tempuyung (Sonchus arvensis) dan Salam (Syzygium polyanthum) Pada Mencit Hiperurisemia. Jurnal Biomedika, 4(1), 17–23.

Mustapa, A. M., Muhammad, T., & Friskawati, H. (2019). Uji Praklinik Kombinasi Obat Herbal Kopi Pinogu (Coffea canephora var Robusta) dan Bunga Cengkeh (Syzygium aromaticum) Terhadap Penurunan Kadar Asam Urat pada Mencit. Ejournal Poltektegal, 8(2), 14–20.

Rinayanti, A., Rahayu, S., & Syachfitri, R. (2016). Uji Aktivitas Penghambatan Xantin Oksidase secara In-Vitro oleh Isolat 6,4’-Dihidroksi-4-Metoksibenzofenon–2–O–β–D Glukopiranosida (C20H22O10) dari Mahkota Dewa (Phaleria macrocarpa). Pharm Sci Res, 3(1), 1–11.

Sadad, A. R., Ermayanti, A., & Palupi, D. H. S. (2009). Pengaruh Pemberian Air Seduhan Bunga Rosela (Hibiscus Sabdariffa L.) Pada Penurunan Kadar Asam Urat Darah Tikus Putih Jantan Galur Wistar Hiperurisemia. Media Farmasi Indonesia, 4(1), 323–331.

Sihombing, M., & Tuminah, S. (2011). Perubahan Nilai Hematologi, Biokimia Darah, Bobot Organ dan Bobot Badan Tikus Putih pada Umur Berbeda. Jurnal Veteriner, 12(1), 58–64.

Simamora, R. H., & Saragih, E. (2019). Penyuluhan Kesehatan Masyarakat: Penatalaksanaan Perawatan Penderita Asam Urat Menggunakan Media Autovisual. Jurnal Pendidikan dan Pemberdayaan Masyarakat, 6(1), 24–31.

Sonia, R., Yusnelti, Y., & Fitrianingsih, F. (2020). Efektivitas Ekstrak Etanol Daun Durian (Durio zibethinus (Linn.)) sebagai Antihiperurisemia. Jurnal Kefarmasian Indonesia, 10(2), 130–139.

World Health Organization. (2013). A Global Brief On Acid. Geneva: WHO.

Yulianto, D. (2005). Inhibisi Xantin Oksidase secara In Vitro oleh Ekstrak Rosela (Hibiscus Sabdariffa L.) dan Ciplukan (Physalis angulata). Departemen Kimia, Institut Pertanian Bogor.

Permana, D., & Kurniawan, T. (2021). Aktivitas Antihiperurisemia Ekstrak Etanol Daun Salam (Syzygium polyanthum) pada Tikus Wistar. Jurnal Ilmu Kefarmasian Indonesia, 19(1), 45–53.

Hidayat, M., & Arifah, N. (2018). Efektivitas Kombinasi Ekstrak Daun Sirsak dan Daun Salam terhadap Penurunan Asam Urat Tikus Wistar. Jurnal Penelitian Farmasi Indonesia, 7(3), 101–109.

Latifah, R., & Khotimah, H. (2020). Uji Efektivitas Kombinasi Ekstrak Daun Binahong dan Rosela terhadap Penurunan Asam Urat. Jurnal Sains dan Kesehatan, 12(2), 200–207.

Nugroho, A. E., & Sari, K. P. (2017). Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sambiloto (Andrographis paniculata) pada Mencit. Pharmacon, 18(1), 8–14.

Wulandari, D., & Sudarmono, P. (2022). Peran Flavonoid dalam Tanaman Obat sebagai Inhibitor Xantin Oksidase: Kajian Literatur. Jurnal Kimia Kesehatan, 10(1), 22–29.

Putra, M. P., & Lestari, D. (2021). Review: Senyawa Bioaktif dari Tumbuhan dalam Pengelolaan Hiperurisemia. Jurnal Fitofarmaka Indonesia, 8(1), 40–49.




DOI: https://doi.org/10.33394/bioscientist.v13i2.16594

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