Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1)
Abstract
Xylocopa appendiculata is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Keywords
Full Text:
PDFReferences
Anisa., Jaya, A.K., & Sunarti. (2016). Analisis Hidden Markov Model untuk Segmentasi Barisan DNA. Jurnal Matematika, Statistika dan Komputasi, 13(1):55-65. https://doi.org/10.20956/jmsk.v13i1.3484
Dahlberg, L., Hauser, M., & Yanega, D. (2013). Japanese Carpenter Bee, Xylocopa appendiculata Smith 1852 (Hymenoptera: Apidae) Potentially Established in Santa Clara County, first record for North America. Pan-Pacific Entomologist, 89(4):226-229. https://doi.org/10.3956/2013-22.1
Devy, S., Astarini, I.A., Putra, I.N.G., Sembiring, A., Yusmalinda, L.A., Malik, M.D.A, & Pertiwi, N.P.D. (2021). Keragaman Genetik Ikan Tongkol Abu-Abu (Thunnus tonggol) yang Didaratkan di Pasar Ikan Sagulung, Batam, Kepulauan Riau Berdasarkan DNA Mitokondria. Journal of Marine and Aquatic Sciences, 7(2): 176-183. https://doi.org/10.24843/jmas.2021.v07.i02.p06
Farook, U.B., Yaqoob, M., Gani, M., Mughal, N., Jeelani, F., & Wani, T.A. (2022). Floral Resources and Foraging Ecology of Large Carpenter (Xylocopa valga, Xylocopa pubesence, Xylocopa fenestrata and Xylocopa dejeanii) of Jammu and Kashmir and Ladakh region. The Pharma Innovation Journal, 11(11):1411-1414. https://www.researchgate.net/publication/365451218_Floral_resources_and_foraging_ecology_of_large_carpenter_bees_of_Jammu_and_Kashmir_and_Ladakh_region
Fietri, W.A., Razak, A., & Ahda, Y. (2020). Analisis Filogenetik Ikan Tuna (Thunnus spp) di Perairan Maluku Utara Menggunakan COI (Cytocrome Oxydase I). Jurnal Biologi Makasar, 5(1):69-78. https://doi.org/10.20956/bioma.v6i2.14053
Giannini, T.C., Alves, D.A., Alves, R., Cordeiro, G.D., Campbell, A.J., Awade, M., Bento, J.M.S., Saraiva, A.M., & Fonseca, V.L.I. (2020). Unveiling the contribution of bee pollinators to Brazilian crops with implications for bee management. Apidologie, 51(3):406-421. https://doi.org/10.1007/s13592-019-00727-3
Hendiari, I.G.A.D., Sartimbul, A., Arthana, I.W., & Kartika, G.R.A. (2020). Keragaman Genetik Ikan Lemuru (Sardinella lemuru) di Wilayah Perairan Indonesia. Acta Aquatica: Aquatic Sciences Journal, 7(1):28-36.
https://doi.org/10.29103/aa.v7i1.2405
Hermawan, C. (2023). Analisis Filogeni Kura-Kura Batok (Cuora amboinensis) Wilayah Indonesia Timur (Ambon, Luwu, Dan Gorontalo) Berbasis Sekuen Gen Cytochrome B. Jurnal Biosense, 6(1):26-46. https://doi.org/10.36526/biosense.v6i01.2602
Hermawan, I., Amin, M., & Suhadi. (2022). Genetic Diversity of Springtails (Collembola Subclass) Based on Cytochrome oxidase Subunit I (COI) Genes in Malang. Biotropika: Journal of Tropical Biology, 10(1):67-77. https://doi.org/10.21776/ub.biotropika.2022.010.01.09
Hidayat, P.A., Pratiknyo, H., & Basuki, D.E. (2016). Keragaman Serangga Polinator pada Tumbuhan Edelweiss Jawa (Anaphalis javanica) di Gunung Slamet Jawa Tengah. Seminar Nasional Pendidikan dan Saintek, 2016:481-491. https://proceedings.ums.ac.id/snpbs/article/view/515/511
Idami, Z. (2024). Identifikasi DNA Barcoding Tumbuhan Balakka (Phyllanthus emblica L.) berdasarkan Lokus Gen rbcL. Biogenerasi. 10(1):541-546. https://doi.org/10.30605/biogenerasi.v10i1.5145
Kamal, M.M., Hakim, A.A., Butet, N.A., Fitrianingsih, Y., & Astuti, R. (2019). Autentikasi Spesies Ikan Kerapu berdasarkan Marka Gen Mt-Coi dari Perairan Peukan Bada, Aceh. Jurnal Biologi Tropis, 19(2):116-123. https://doi.org/10.29303/jbt.v19i2.1245
Kang, A.R., Kim, M.J., Park, I.A., Kim, K.Y., & Kim, I. (2016). Extent and Divergence of Heteroplasmy of the DNA Barcoding Region in Anapodisma miramae (Orthoptera: Acrididae). Mitochondrial DNA, 27(5):3405-3414. https://doi.org/10.3109/19401736.2015.1022730
Kawazoe, K., Kawakita, A., Kameda, Y., & Kato, M. (2008). Redundant Species, Cryptic Host-Associated Divergence, and Secondary Shift in Sennertia Mites (Acari: Chaetodactylidae) Associated With Four Large Carpenter Bees (Hymenoptera: Apidae: Xylocopa) in the Japanese Island arc. Molecular Phylogenetics and Evolution, 49(2):503-513.
https://doi.org/10.1016/j.ympev.2008.07.024
Marchiori, C.H. (2023). Verification of The peculiarities of The Anthophoridae Family (Insecta: Hymenoptera). World Journal of Biological and Pharmaceutical Research, 5(1):015-027. https://doi.org/10.53346/wjbpr.2023.5.1.0059
Mohapatra, S.K., Nayak, V.K., Paul, A., & Adhikary, S. (2019). Mitochondrial DNA: A Molecular Tool for Assessment of Genetic Diversity. International Journal of Livestock Research, 9(8):49-58. https://doi.org/10.5455/ijlr.20190404113609
Pangestika, Y., Budiharjo, A., & Kusumaningrum, H.P. (2015). Analisis Filogenetik Curcuma Zedoaria (Temu Putih) Berdasarkan Gen Internal Transcribed Spacer (Its). Jurnal Biologi, 4(4):8-13.
https://ejournal3.undip.ac.id/index.php/biologi/article/view/19424
Partiwi, S., Idrus, A.A., Zulkifli, L., Mahrus., & Sedijani, P. (2023). Isolation and Molecular Characterization of Brotowali (Tinospora crispa) Rhizosphere Bacteria Producing Siderophore from Dry Lands of Lombok Island. Jurnal Biologi Tropis, 23(2):275-284. https://doi.org/10.29303/jbt.v23i2.6138
Pujiastuti, Y., & Putri, K.U. (2021). Keanekaragaman Spesies Lebah (Hymenoptera : Apidae) pada Berbagai Jenis Tanaman Berbunga di Kota Pagaralam Sumatera Selatan. Seminar Nasional Lahan Suboptimal, 9(2021):603-610. https://conference.unsri.ac.id/index.php/lahansuboptimal/article/view/2428/1396
Sahadeva, M.L., & Pertiwi, N.P.D. (2023). Konstruksi Pohon Filogenetik Spesies dalam Famili Orchidaceae Berdasarkan Marka Gen matK Kloroplas : Studi in Silico. Wahana Matematika dan Sains: Jurnal Matematika, Sains, dan Pembelajarannya, 17(3):12-27. https://doi.org/10.23887/wms.v17i3.87986
Sasaki, K., Goto, K., & Harano, K.I. (2020). Timing of Male Territorial Flight and Foraging of The Large Carpenter Bee Xylocopa appendiculata Related to Serotonin in The Brain. Science of Nature, 107(3):1-9. https://doi.org/10.1007/s00114-020-01681-2
Tindi, M., Mamangkey, N.G.F., & Wullur, S. (2017). The DNA Barcode and Molecular Phylogenetic Analysis Several Bivalve Species from North Sulawesi Waters based on COI gene. Jurnal Pesisir dan Laut Tropis, 1(2):32–38. https://doi.org/10.35800/jplt.5.2.2017.15050
Trianto, M., & Purwanto, H. (2020). Morphological characteristics and morphometrics of stingless bees (Hymenoptera: Meliponini) in Yogyakarta, Indonesia. Biodiversitas, 21(6): 2619–2628. https://doi.org/10.13057/biodiv/d210633
Zhang, H., & Bu, W. (2022). Exploring Large-Scale Patterns of Genetic Variation in the COI Gene among Insecta: Implications for DNA Barcoding and Threshold-Based Species Delimitation Studies. Insects, 13(5):1-11. https://doi.org/10.3390/insects13050425
DOI: https://doi.org/10.33394/bioscientist.v13i1.14969
Refbacks
- There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Bioscientist : Jurnal Ilmiah Biologi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
Editorial Address: Pemuda Street No. 59A, Catur Building Floor I, Mataram City, West Nusa Tenggara Province, Indonesia