Email this article Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif
Abstract
This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
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Andayani, I. G. A. S., Sulastri, S., Hananto, D. A., & Sriasih, M. (2020). Ekstrak daun jati (Tectona grandis) alternatif pewarna pada penghitungan jumlah dan viabilitas sel kultur dibandingkan dengan pewarna tryphan blue. Bioscientist: Jurnal Ilmiah Biologi, 8(2), 205–211.
Agus, A., Hanim, C., Al Anas, M., & Agussalim, A. (2022). Feed, animal and human health: Designing functional egg. In Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021) (Vol. 21, pp. 313–319). https://doi.org/10.2991/absr.k.220401.065
Almeida, N. M., Dias, F. F. G., Rodrigues, M., & Moura Bell, J. L. (2019). Effects of processing conditions on the simultaneous extraction and distribution of oil and protein from almond flour. Processes, 7(844). https://doi.org/10.3390/pr7110844
Anton, M., Castellani, O., & Guérin-Dubiard, C. (2007). Phosvitin. In Bioactive Egg Compounds (pp. 17–24). Springer. https://link.springer.com/book/10.1007/978-3-540-37885-3#page=21
Badan Pengawas Obat dan Makanan (BPOM). (2011). Klaim dalam label & iklan pangan olahan. https://standarpangan.pom.go.id/dokumen/peraturan/2011/2011HK.03.1.23.11.11.09909-Pengawasan
Betschart, M. U., Sarem, M., Shastri, V. P., & Lüdeke, S. (2022). Reversible, β-sheet-dependent self-assembly of the phosphoprotein phosvitin is controlled by the concentration and valency of cations. Physical Chemistry Chemical Physics, 19. https://doi.org/10.1039/D1CP05493G
Castellani, O., Martinet, V., David-Briand, E., Guérin-Dubiard, C., & Anton, M. (2003). Egg yolk phosvitin: Preparation of metal-free purified protein by fast protein liquid chromatography using aqueous solvents. Journal of Chromatography B, 791(1–2), 273–284. https://doi.org/10.1016/S1570-0232(03)00230-7
Erni, N., Kadirman, & Ratnawaty. (2018). Pengaruh suhu dan lama pengeringan terhadap sifat kimia dan organoleptik tepung umbi talas (Colocasia esculenta). Jurnal Pendidikan Teknologi Pertanian, 4, 95–105.
Fitriyani, N., Hintono, H., & Pramono, Y. B. (2017). Sifat fungsional whole egg hasil freeze drying dengan umur telur yang berbeda. Jurnal Aplikasi Teknologi Pangan, 6(3), 1–4. https://doi.org/10.17728/jatp.205
Hoon, S. (2016). Cytotoxic and antigenotoxic activities of phosvitin from egg yolk. Iowa State University Animal Industry Report.
Ko, K. Y., Nam, K. C., Jo, C., Lee, E. J., & Ahn, D. U. (2011). A simple and efficient method for preparing partially purified phosvitin from egg yolk using ethanol and salts. Poultry Science, 90(5), 1096–1104. https://doi.org/10.3382/ps.2010-01138
Lesnierowski, G., & Stangierski, J. (2018). What’s new in chicken egg research and technology for human health promotion? A review. Trends in Food Science & Technology, 71, 46–51. https://doi.org/10.1016/j.tifs.2017.10.022
Lilienthal, S., Drotleff, A. M., & Ternes, W. (2015). Changes in the protein secondary structure of hen’s egg yolk determined by Fourier transform infrared spectroscopy during the first eight days of incubation. Poultry Science, 94, 68–79. https://doi.org/10.3382/ps/peu051
Liu, Y., & Liu, X. (2022). Effect of four extraction processes with acetone-ethanol protocols on the properties of egg yolk phosphatidylcholine and the structure of residual protein. Journal of Oleo Science, 72(1), 49–58. https://doi.org/10.5650/jos.ess22219
Marcet, I., Ridella, F., Díaz, M., & Rendueles, M. (2023). New processes to extract and purify phosvitin by using aqueous salt solutions, precipitation and ultrafiltration techniques. Separations, 10(407). https://doi.org/10.3390/separations10070407
Marcet, I., Sáez-Orviz, S., Rendueles, M., & Díaz, M. (2022). Egg yolk granules and phosvitin: Recent advances in food technology and applications. LWT - Food Science and Technology, 153, 112442. https://doi.org/10.1016/j.lwt.2021.112442
Moreno, J. A., Díaz-Gómez, J., Fuentes-Font, L., Angulo, E., Gosálvez, L. F., Sandmann, G., Portero-Otin, M., Capell, T., Zhu, C., Christou, P., & Nogareda, C. (2020). Poultry diets containing (keto)carotenoid-enriched maize improve egg yolk color and maintain quality. Animal Feed Science and Technology, 260, 114334. https://doi.org/10.1016/j.anifeedsci.2019.114334
Nasution, L. M. (2017). Statistik deskriptif. Jurnal Hikmah, 14(1), 49–55.
Nurhaerani, Wariata, W., Kisworo, D., & Depamede, S. N. (2019). Purification of polyclonal antibody against pork extracts antigens using protein A column as material for developing halal food detection kit. AIP Conference Proceedings, 2019(9), 70012–70015. https://doi.org/10.1063/1.5141328
Duffuler, P., Giarratano, M., Naderi, N., Suwal, S., Marciniak, A., Perreault, V., Offret, C., Brisson, G., House, J. D., Pouliot, Y., & Doyen, A. (2020). High hydrostatic pressure induced extraction and selective transfer of β-phosvitin from the egg yolk granule to plasma fractions. Food Chemistry, 321, 126696. https://doi.org/10.1016/j.foodchem.2020.126696
Ren, J., & Wu, J. (2014). Preparation of high purity egg phosvitin using anion exchange chromatography. Food Chemistry, 158, 186–191. https://doi.org/10.1016/j.foodchem.2014.02.120
Ternes, W. (1989). Characterization of water-soluble egg yolk proteins with isoelectric focusing. Journal of Food Science, 54, 764–765. https://doi.org/10.1111/j.1365-2621.1989.tb04704.x
Wulandari, Z., & Arief, I. I. (2022). Review: Tepung telur ayam: Nilai gizi, sifat fungsional dan manfaat. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 10(2), 62–68. https://doi.org/10.29244/jipthp.10.2.62-68
Xin, X., Zhang, G., Xue, H., Qiu, W., Hu, H., Tu, Y., & Zhao, Y. (2022). Effects of ethanol treatment on the physicochemical properties, microstructure and protein structures of egg yolk gels. Food Chemistry, 405(Pt B), 135041. https://doi.org/10.1016/j.foodchem.2022.135041
Yilmaz, B., & Agagunduz, D. (2020). Bioactivities of hen’s egg yolk phosvitin and its functional phosphopeptides in food industry and health. Journal of Food Science, 85(10), 2969–2976.
Zhang, X., Huang, X., & Ma, M. (2017). Role of phosphorylation of phosvitin in the phase transformation of mineralization. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2017.03.158
DOI: https://doi.org/10.33394/bioscientist.v13i2.14304
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