Email this article Filogenetik Ikan Kuro (Eleutheronema sp.) di Perairan Pesisir Merauke, Papua Selatan
Abstract
This research aims to identify kuro fish (Eleutheronema sp.) collected from the coastal waters of Merauke. Cytochrome Oxidase Subunit I (COI) Gene was amplified by the Polymerase Chain Reaction (PCR) method, using primers FISH F1 (5’-TCA ACC AAC CAC AAA GAC ATT GGC AC-3’) and FISH R1. (5’-TAG ACT TCT GGG TGG CCA AAG AAT CA -3’), while DNA data analysis uses Mega 11 software. Results research shows that DNA sequence obtained were 675 base pairs long with the identification result being Eleutheronema tetradactylum with a similarity level of 99.54 and 99.85% for each sample. Based on phylogenetic analysis and genetic distance, the two DNA sequences analyzed form the same clade with identification results with a genetic distance of 0.001. Analysis of genetic diversity found 2 haplotypes from the two samples. Both the Sample Have a small genetic distance because THEY are collected from the same waters DNA barcoding and phylogenetics can be used to identify species for management and conservation of species.
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Abdullah, A., Sativa, H. A., Nurhayati, T., & Nurilmala, M. (2019). DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 508–519. https://doi.org/10.17844/jphpi.v22i3.28950.
Dailami, M., Rahmawati, A., Saleky, D., & Toha, A. H. A. (2021). DNA barcoding of tilapia fish from Merauke , Papua and Malang , East Java-Indonesia. AACL Bioflux:14(2), 849–858.
Fietri, W. A., Razak, A., & Ahda, Y. (2020). Analisis Filogenetik Ikan Tuna (Thunnus spp) di Perairan Maluku Utara Menggunakan COI (Cytocrome Oxydase I). Jurnal Biologi Makasar, 5(1), 69–78.
Hajisamae, S., Iqbal, T.H., Soe, K.Y., Wang, W., (2023). Phenotypic variation of protandrous hermaphrodite fish Eleutheronema tetradactylum (Shaw, 1804) from Thailand coastal waters, Journal of Sea Research, 196 (102448) 1-12, https://doi.org/10.1016/j.seares.2023.102448.
Halisah, K. A. Z., Fitri, A., Irianto, S.N, Aulya, M., Sakti, B., Harmadi, P., Sukma Maha, N. S.,Cahyani, K. D. (2024). Identifikasi Spesies Ikan Laut Menggunakan Teknik DNA Barcoding. Bioma: Berkala Ilmiah Biologi, 26(1), 43–51.
Horne,J.B., Momigliano,P., Welch,D.J., Newman,S.J. and van Herwerden,L. (2011). Limited ecological population connectivity suggests low demands on self-recruitment in a tropical inshore marine fish (Eleutheronema tetradactylum: Polynemidae). Mol. Ecol. 20 (11), 2291-2306.
Gebze, A., Latupeirissa, I. (2017). Pertumbuhan ikan kuro (Eleutheronema tetradactylum Shaw, 1804) di muara Sungai Kumbe Kabupaten Merauke. Agricola: 1 (7), 129-135.
Jefri Permadi, Ana Rochvita, Rr. Citra Permata Kusuma Anggraini, & Flandrianto Sih Palimirmo. (2022). Filogenetik Ikan Ekstremofil Edible Populasi Magelang Menggunakan Gen Cytochrome Oxydase I. Journal of Research and Technology, 8(1). https://doi.org/10.55732/jrt.v8i1.507.
Kholis, M. N., Wahju, R. I., . M., & . J. (2018). Struktur Ukuran Dan Hubungan Panjang Berat Ikan Kurau Di Pulau Bengkalis. ALBACORE Jurnal Penelitian Perikanan Laut, 2(2), 197–208. https://doi.org/10.29244/core.2.2.197-208.
Kusmita, R., Sunarni., Saleky, D., (2024). Pertumbuhan dan mortalitas Ikan Kuro (Eleutheronema tetradactylum) yang didaratkan di pelabuhan perikanan nusantara Kabupaten Merauke. Bioscientist : Jurnal Ilmiah Biologi. 12(1): 305-316.
Lelono, T. D., Tumulyadi, A., Sari, W. K., & Ismaningsih, I. S. (2021). Biological Aspects of Fourfinger Threadfin, Eleutheronema tetradactylum (Shaw, 1804) Caught in Lekok Waters, Pasuruan, East Java. Journal of Enviromental Engineering and Sustainable Technology, 8(2), 21–27. https://doi.org/10.21776/ub.jeest.2021.008.02.1.
Morihito, R. V. S. ., Chungdinata, S. E., Nazareth, T. A., Pulukadang, M. I., Makalew, R. A. ., & Pinontoan, B. (2017). Identifikasi Perubahan Struktur DNA Terhadap Pembentukan Sel Kanker Menggunakan Dekomposisi Graf. Jurnal Ilmiah Sains, 17(2), 153. https://doi.org/10.35799/jis.17.2.2017.17368
Qu, Z., W. Nong, Y. Yu, T. Baril, H.Y. Yip, A. Hayward, J.H. Hui. (2020) Genome of the four-finger threadfin Eleutheronema tetradactylum (perciforms: Polynemidae). BMC Genomics, 2:1-10.
Saleky, D., Leatemia, Simon P.O Pattiasina, T. F., Isma, Rosa, P., Welliken, M. A., & Melmambessy, Edy H.P Dailami, M. (2020). Analisis pola pertumbuhan dan pendekatan dna barcoding untuk identifikasi Turbo stenogyrus P. Fischer, 1873 (Mollusca: Gastropoda). Biotropika - Journal of Tropical Biology, 8(2), 79–86. https://doi.org/10.21776/ub.biotropika.2020.008.02.03.
Saleky, D., & Merly, S. L. (2021). Pendekatan DNA Barcoding untuk Identifikasi Cassidula angulifera (Petit, 1841) (Moluska: Gastropoda). Jurnal Sumberdaya Akuatik Indopasifik, 5(1), 55–64.
Saleky, D., & Dailami, M. (2021). Konservasi Genetik Ikan Kakap Putih (Lates calcarifer, Bloch, 1790) Melalui Pendekatan DNA Barcoding dan Analisis Filogenetik di Sungai Kumbe Merauke Papua. Jurnal Kelautan Tropis, 24(2), 141–150. https://doi.org/10.14710/jkt.v24i2.10760.
Sharma, N., Pasala, M. S., & Prakash, A. (2019). Mitochondrial DNA: Epigenetics and environment. Environmental and molecular mutagenesis, 60(8), 668-682.
Shihab, I.,A. Gopalakrishnan, N. Vineesh, M. Muktha, K.V. Akhilesh, P. Vijayagopal., (2017). Histological profiling of gonads depicting protandrous hermaphroditism in Eleutheronema tetradactylum. J. Fish Biol., 90 (6): 2402-2411.
Sianturi, R., Dailami, M., & Saleky, D. (2021). Identifikasi dan Analisis Filogenetik Ikan Ekonomis Penting Oreochromis sp. dengan Pendekatan DNA Barcoding. Bioscientist : Jurnal Ilmiah Biologi, 9(2), 465. https://doi.org/10.33394/bioscientist.v9i2.4027.
Siriwut, W., Jeratthitikul, E., Panha, S., Chanabun, R., & Sutcharit, C. (2020). Molecular phylogeny and species delimitation of the freshwater prawn Macrobrachium pilimanus species group, with descriptions of three new species from Thailand. PeerJ, 8. https://doi.org/10.7717/peerj.10137
Soe, K.K S. Pradit, S. Hajisamae. (2021). Feeding habits and seasonal trophic guilds structuring fish community in the bay mouth region of a tropical estuarine habitat. J. Fish Biol., 99 (4): 1430-1445.
Sritakon, T., R. Rungnapa Nooklum, S. Vibunpant, N. Niracha Songkaew, T. Kongchai. (2019). Reproductive Biology of Fourfinger Threadfin (Eleutheronema tetradactylum (Shaw, 1804)) in Nakhon Si Thammarat and Songkhla provinces. Technical Paper No. 23/2019
Sunarni., Maturbongs, M.R., (2018). Pertumbuhan dan tingkat kematangan gonad ikan kuro (Eleutheronema tetradactylum) di daerah estuari pesisir pantai Kota Merauke. Musamus Fisheries and Marine Journal, 1 (1): 15-23.Tamura K, Stecher G, and Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution 38:3022-3027.
Twindiko, A. F. S., Wijayanti, D. P., & Ambariyanto. (2013). Genus Pseudochromis Dan Pictichromis. Buletin Oseanografi Marini, 2, 28–36.
Ude, G. N., Igwe, D. O., Brown, C., Jackson, M., Bangura, A., Ozokonkwo-Alor, O., Ihearahu, O. C., Chosen, O., Okoro, M., Ene, C., Chieze, V., Unachukwu, M., Onyia, C., Acquaah, G., Ogbonna, J., & Das, A. (2020). DNA barcoding for identification of fish species from freshwater in Enugu and Anambra States of Nigeria. Conservation Genetics Resources, 12(4), 643–658. https://doi.org/10.1007/s12686-020-01155-7.
Ward, R., Zemlak, T., Innes, B., Last, P., & Hebert, P. (2005). DNA barcoding Australia&apos. Philos Trans R Soc Lond B, 360 (1462): 1847-1857.
Ward, R. D., Holmes, B. H., & Yearsley, G. K. (2008). DNA barcoding reveals a likely second species of Asian sea bass (barramundi) (Lates calcarifer). Journal of Fish Biology, 72(2), 458–463. https://doi.org/10.1111/j.1095-8649.2007.01703.x.
Xiao, J., Lyu, S., Iqbal, T. H., Hajisamae, S., Tsim, K. W. K., & Wang, W. X. (2022). Molecular phylogenetic and morphometric analysis of population structure and demography of endangered threadfin fish Eleutheronema from Indo-Pacific waters. Scientific Reports, 12(1), 1–11. https://doi.org/10.1038/s41598-022-07342-w
Xiao, J., Karl W.K. Tsim, Sukree Hajisamae, Wen-Xiong Wang. (2023). Chromosome-level genome and population genomics provide novel insights into adaptive divergence in allopatric Eleutheronema tetradactylum, International Journal of Biological Macromolecules, 244, 125299, ISSN 0141-8130,https://doi.org/10.1016/j.ijbiomac.2023.125299.
DOI: https://doi.org/10.33394/bioscientist.v13i2.13685
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