Induksi Kalus Krisan (Chrysanthemum morifolium var. reagen pink) dengan Penambahan Naphtalen Acetic Acid (NAA) dan Kinetin Secara In-Vitro

Washeilatus Sholehah, Ruri Siti Resmisari, Safina Oktafia, Siwi Putri Mumpuni

Abstract


Chrysanthemum morifolium var. reagent pink is an ornamental flower that is widely cultivated in Indonesia. The increasing demand makes it difficult for farmers to fulfill the market. In-vitro tissue culture is an effective solution to multiply chrysanthemum seedlings in bulk and quickly. This study aims to identify the most effective concentration in inducing callus in chrysanthemum plants by combining Naphtalen Acetic Acid (NAA) and kinetin. This study was an experimental research with a completely randomized design (CRD), involving 12 treatments and each treatment was repeated 4 times. There were two treatment factors; NAA concentration (0 mg/l, 0.5 mg/l, 1 mg/l) and kinetin concentration (0 mg/l, 1 mg/l, 2 mg/l, 3 mg/l). Parameters in this study included days to callus appearance, percentage of callus explants, callus wet weight, callus color, and callus texture. Data were analyzed using analysis of variance (ANOVA). The results showed that (1) the fastest callus was formed at 11 HST by giving the treatment of 0 mg/l NAA + 3 mg/l kinetin; (2) the optimal treatment for the percentage of callus explants was obtained by giving 1 mg/l NAA + 1 mg/l kinetin); (3) the optimal treatment of callus wet weight was obtained by giving 1 mg/l NAA + 2 mg/l kinetin; (4) the best callus quality was obtained by giving a combination of NAA 1 mg/l and kinetin 1 mg/l, with a crumbly texture and brownish yellow callus.

Keywords


Chrysanthemum morifolium var. reagen pink, NAA, kinetin, callus

Full Text:

PDF

References


Angelina, N., Siregar, L. A. M., & Putri, L. A. P. (2017). Pengaruh Zat Pengatur Tumbuh Terhadap Induksi Akar (Rhizogenesis) Pada Tanaman Bangun-Bangun (Plectranthus Amboinicus (Lour.) Spreng) secara In Vitro. Jurnal Agroekoteknologi FP USU, 5(3), 644–649.

Anwar, N., & Isda, M. N. (2021). Respons Pembentukan Kalus Daun Pegagan (Centella asiatica (L.) Urb.) dengan Penambahan Naphtalene Acetic Acid dan Benzyl Amino Purin Secara In Vitro. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 5(2), 136–142. https://doi.org/10.24002/biota.v5i3.3232

Asra, R., Samarlina, R. A., & Silalahi, M. (2020). Hormon Tumbuhan. In UKI Press 53(9). http://repository.uki.ac.id/1579/1/Hormon Tumbuhan.pdf

Dakah, A., Zaid, S., Suleiman, M., Abbas, S., & Wink, M. (2014). In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences, 21(4), 317–323. https://doi.org/10.1016/j.sjbs.2013.12.002

Desy Puspitasari. (2023). Krisan Nasional Siap Menggantikan Krisan Introduksi. Diakses 4 September 2024, https://hortikultura.pertanian.go.id/krisan-nasional-siap-menggantikan-krisan-introduksi/

Ekawati, Y., Anggraeni, A., Dyah Prawestri, A., & Nurtjahya, E. (2022). Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro. AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian, 6(2), 28–37. https://doi.org/10.33019/agrosainstek.v6i2.316

Indrajati, S., Saputro, L., & Yuniar, A. (2023). Panduan Teknik Budidaya Krisan Potong.

Kaviani, B., Hesar, A. A., Tarang, A., & Zanjani, S. B. (2013). Effect of kinetin (Kn) and naphthalene acetic acid (NAA) on the micropropagation of Matthiola incana using shoot tips, and callus induction and root formation on the leaf explants. African Journal of Agricultural Research, 8(30), 4134–4139. https://doi.org/10.5897/AJAR11.921

Krestiani, V., & Rukmi. (2013). Kajian konsentrasi NAA dan kinetin terhadap pertumbuhan kalus dari kotiledon sambiloto ( Andrographis paniculata Ness .) secara in vitro. Jurnal Sains dan Teknologi, 6(1), 16–20.

Lengkong, E. F., Mantiri, H., & Pinaria, A. G. (2023). Growth Of Potato Seeds (Solanum tuberesum L.) On Ms Media Substituted With Coconut Water. Jurnal Agroekoteknologi Terapan, 4(2), 361–369. https://doi.org/10.35791/jat.v4i2.50675

Lizawati. (2012). Induksi Kalus Embriogenik Dari Eksplan Tunas Apikal Tanaman Jarak Pagar (Jatropha curcas L.) Dengan Penggunaan 2,4 D Dan Tdz. Journal Bioplantae, 1(2), 75–87.

Millenia, F. K., Suminar, E., Nuraini, A., & Pitaloka, G. G. (2022). Induksi Kalus Eksplan Daun Stroberi ( Fragaria x ananassa Duch ) dengan Pemberian NAA dan CaP Secara In Vitro Callus. Jurnal Galung Tropika, 11(3), 317–329. https://doi.org/10.31850/jgt.v11i3.1023

Mufarrikha, L., Herlina, N., & Widaryanto, E. (2014). Respon Dua Kultivar Tanaman krisan (Chrysanthemum morifolium) Pada Berbagai Lama Penambahan Cahaya Buatan. Jurnal Produksi Tanaman, 2(1), 10–16.

Muliati, Tengku Nurhidayah, N. (2017). Media On The In Vitro Development Of Sansevieria macrophylla. Jom Faperta, 4(1), 1–13.

Nuha. A. A. (2022). Pengaruh Berbagai Konsentrasi NAA dan BAP Terhadap Induksi Kalus Daun Porang (Amorphopallus muelleri Blume) Secara In-Vitro. Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Purnamaningsih, R., & Ashrina, M. (2011). Pengaruh Bap Dan Naa Terhadap Induksi Kalus Dan Kandungan Artemisinin Dari Artemisia annua L. 1 [The Effect of BAP and NAA on Callus Induction and Artemisinin Content of Artemisia annua L.]. Berita Biologi, 10(4), 481–489.

Rahayu, B., Solichatun, & Anggarwulan, E. (2003). Pengaruh Asam 2,4-Diklorofenoksiasetat (2,4-D) terhadap Pembentukan dan Pertumbuhan Kalus serta Kandungan Flavonoid Kultur Kalus Acalypha indica L. The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on callus growth and production flavonoid content on c. Biofarmasi, 1(1), 11.

Rasud, Y., & Bustaman, B. (2020). In Vitro Callus Induction from Clove (Syzigium aromaticum L.) Leaves on Medium Containing Various Auxin Concentrations. Jurnal Ilmu Pertanian Indonesia, 25(1), 67–72. https://doi.org/10.18343/jipi.25.1.67

Restu Dinika, A., Widyodaru Saputro, N., Sulandjari, K., & Rahmi, H. (2021). Organogenesis Kalus Tanaman Krisan (Chrysanthemum indicum L.) Dengan Penggunaan Kinetin Dan NAA (Naphthalene Acetic Acid). Jurnal Agrium, 18(1), 72–79. https://doi.org/10.29103/agrium.v18i1.3845

Sari, M., & Isda, M. N. (2021). The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro. Jurnal Biologi UNAND, 9(1), 8. https://doi.org/10.25077/jbioua.9.1.8-17.2021

Sari, P. K., Rosanti, D., & Putri, Y. P. (2022). Karakteristik Tanaman Hias Pekarangan Rumah di Kelurahan Plaju Ulu Kota Palembang. Indobiosains, 4(1), 15. https://doi.org/10.31851/indobiosains.v4i1.6199

Siregar, A. Z., & Lesnida, S. (2021). Pemanfaatan Tanaman Refugia Mengendalikan Hama Padi (Oryza nivara L) Di Soporaru Tapanuli Utara. Agrifor, 20(2), 299. https://doi.org/10.31293/agrifor.v20i2.5744

Sosnowski, J., Truba, M., & Vasileva, V. (2023). The Impact of Auxin and Cytokinin on the Growth and Development of Selected Crops. Agriculture (Switzerland), 13(3), 1–14. https://doi.org/10.3390/agriculture13030724

Sudrajad, H., & Wijaya, N. R. (2019). Pengaruh Kinetin Dan Naa Terhadap Induksi Kalus Pule Pandak (Rauvolfia serpentina (L.) Benth. ex Kurz). Jurnal Tumbuhan Obat Indonesia, 12(2), 68–74. https://doi.org/10.22435/jtoi.v12i2.1691

Tarigan, S. D. S., Astarini, I. A., & Astiti, N. P. A. (2023). Inisiasi Kalus Bangle (Zingiber purpureum Roscoe) pada Beberapa Kombinasi 2.4-D dan Kinetin. Jurnal Hortikultura Indonesia, 14(2), 93–99. https://doi.org/10.29244/jhi.14.2.93-99

Taslim, M., Mailoa, M., & Rijal, M. (2017). Pengaruh pH, Dan Lama Fermentasi Terhadap Produksi Ethanol Dari Sargassum crassifolium. Jurnal Biology Science & Education, 6(2), 13–25.




DOI: https://doi.org/10.33394/bioscientist.v12i2.13217

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Creative Commons License
Bioscientist : Jurnal Ilmiah Biologi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License

Editorial Address: Pemuda Street No. 59A, Catur Building Floor I, Mataram City, West Nusa Tenggara Province, Indonesia