Gambaran Histopatologi Kulit Ikan Kerapu Cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) yang Terinfestasi Zeylanicobdella arugamensis Setelah Perendaman Menggunakan Perasan Daun Pepaya (Carica papaya)

Bernathdo Mahendra Robbi Putra, Gunanti Mahasri, Laksmi Sulmartiwi

Abstract


Increasing cultivation activities and intensifying ponds has the potential to give rise to problems, namely increasing cases of disease in grouper fish, namely Zeylanicobdella, causing mass deaths of up to 100%. This study aims to analyze the histopathological picture of grouper skin infested with Zeylanicobdella arugamensis after administering papaya leaf juice with different soaking times and concentrations of papaya leaf juice. The method used was experimental Completely Randomized Design (CRD) with a factorial pattern consisting of two factors, namely factor A, different concentrations of papaya leaf juice consisting of 0% (A1), 0.1% (A2), 0.3% (A3) , and 0.5% (A4), as well as factor B for grouper rearing time consisting of the 0th minute (B1), 60th minute (B2), and 120th minute (B3) with five repetitions. The results of the study showed that the soaking time of papaya leaf juice in grouper fish had an effect on reducing the degree of Zeylanicobdella infestation, there were changes in the histopathological picture and water quality values before and after soaking. Treatments A2, A3, and A4 experienced a decrease in the degree of ectoparasite infestation at 60 and 120 minutes of immersion. The histopathological scoring values for treatments A2, A3, and A4 decreased at 60 and 120 minutes of maintenance. The best treatment combination was 0, 3% and soaking time at 120 minutes. In conclusion, giving papaya leaf juice with a soaking time of 120 minutes with a concentration of 0.3% can reduce the degree of infestation and the histopathological scoring value of Zeylanicobdella in grouper fish.

Keywords


Zeylanicobdella arugamensis, Carica papaya, Immunostimulant, Histopathological Slides.

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References


A’yun, Q., & Laily, A. N. (2015). Analisis Fitokimia Daun Pepaya (Carica papaya L.) di Balai Penelitian Tanaman Aneka Kacang dan Umbi, Kendalpayak, Malang. In Seminar Nasional Konservasi dan Pemanfaatan Sumber Daya Alam (pp. 134-137). Malang, Indonesia: Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Agustiya, R., Mahasri, G., & Subekti, S. (2023). Identifikasi Morfometrik dan Intensitas Lintah Laut Zeylanicobdella yang Menginfestasi Ikan Kerapu Cantang Asal Kecamatan Brondong, Lamongan dan Pulau Mandangin, Madura. Journal of Marine and Coastal Science, 12(1), 34-42. https://doi.org/10.20473/jmcs.v12i1.38273

BPS. (2020). Retrieved January 15, 2024, from BPS. Intractwebsite: https://jatim.bps.go.id/statictable/2023/03/02/2440/produksi-dan-nilai-produksi-perikanan-budidaya-kerapu-udang-dan-ikan-lainnya-menurut-kabupaten-kota-dan-komoditas-utama-di-provinsi-jawa-timur-2020-.html

Dahlia., Suprapto, H., & Kusdarwati, R. (2017). Isolasi dan Identifikasi Bakteri pada Benih Ikan Kerapu Cantang (Epinephelus sp.) dari Kolam Pendederan Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo, Jawa Timur. Journal of Aquaculture and Fish Health, 6(2), 57-66.

Firdaus, R. F., Lim, L. S., Kawamura, G., & Shapawi, R. (2016). Assessment on the Acceptability of Hybrid Grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ to Soybean Meal-Based Diets. AACL Bioflux, 9(2), 284-290.

Ghozali, I. (2009). Aplikasi Analisis Multivariate dengan Program SPSS. Semarang: Universitas Diponegoro.

Leknes, I. L. (2015). An Improved Method to Demonstrate Macrophages in Teleosts. Tissue and Cell, 47(3), 254-256. https://doi.org/10.1016/j.tice.2015.02.002

Nisa, M., Mahasri, G., & Sulmartiwi, L. (2021). Gill and Skin Pathology of Hybrid Grouper (E. fuscoguttatus x E. lanceolatus) Infested Zeylanicobdella arugamensis Worms in Different Infestations Degree. IOP Conference Series : Earth and Environmental Science, 679(1), 1-8. https://doi.org/10.1088/1755-1315/679/1/012006

Novriadi, R., & Hendrianto, H. (2015). Penyakit Infeksi pada Budidaya Ikan Kerapu. Batam: Balai Perikanan Budidaya Laut Batam.

Octarina, Y., Prasetiyono, E., Febrianti, D., & Robin. (2018). Efektivitas Ekstrak Daun Ciplukan (Physalis angulata L.) terhadap Sistem Kekebalan Tubuh Ikan Nila (Oreochromis niloticus). Jurnal Riset Akuakultur, 13(3), 259-265. http://dx.doi.org/10.15578/jra.13.3.2018.259-265

Putra, W. K. A., Suhaili., & Yulianto, T. (2020). Efisiensi dan Rasio Konversi Pakan Ikan dengan Berbagai Dosis Papain pada Kerapu Cantang (E. fuscoguttatus >< E. lanceolatus). Jurnal Perikanan Universitas Gadjah Mada, 22(1), 19-26. https://doi.org/10.22146/jfs.55524

Putri, R. D., & Sofyanita, E. N. (2023). Perbedaan Hasil Pewarnaan Hematoxylin Eosin (HE) pada Histologi Kolon Mencit (Mus musculus) Berdasarkan Ketebalan Pemotongan Mikortom 3, 6 dan 9 μm. Jurnal Labora Medika, 7(1), 31-38.

Qomariyah, N., Suprapto, H., & Sudarno, S. (2017). Pemberian Vaksin Formalin Killed Cell (FKC) Vibrio alginolitycus untuk Meningkatkan Survival Rate (SR) Titer Antibodi dan Fagositosis Leukosit pada Kerapu Cantang (Epinephelus sp.) Setelah Uji Tantang Bakteri Vibrio alginolitycus. Jurnal Ilmiah Perikanan dan Kelautan, 9(1), 15-24. https://doi.org/10.20473/jipk.v9i1.7625

Rahayu, N. D., Sulmartiwi, L., Mahasri, G., Muntalim., Angwarmas, B., & Pamenang, G. D. (2020). Inventory of Ectoparasit Helminth on the Hybrid Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) from Traditional Ponds in the Kampung Kerapu Lamongan East Java Indonesia. IOP Conference Series : Earth and Environmental Science, 441(1), 1-7. https://doi.org/10.1088/1755-1315/441/1/012095

Ravi, R., & Yahaya, Z. S. (2017). Zeylanicobdella arugamensis the Marine Leech from Cultured Crimson Snapper (Lutjanus erythropterus), Jerejak Island, Penang, Malaysia. Asian Pacific Journal of Tropical Biomedicine, 7(1), 473-477. https://doi.org/10.1016/j.apjtb.2017.01.018

Sofiana, L., Nofisulastri, N., & Safnowandi, S. (2023). Pola Distribusi Siput Air (Gastropoda) sebagai Bioindikator Pencemaran Air di Sungai Unus Kota Mataram dalam Upaya Pengembangan Modul Ekologi. Biocaster : Jurnal Kajian Biologi, 3(3), 133-158. https://doi.org/10.36312/biocaster.v3i3.191

Wandika, W., Irawan, W., & Raza’I, T. S. (2023). Pemberian Larutan Daun Pepaya (Carica papaya) yang Berbeda terhadap Ektoparasit Lintah (Zeylanicobdella sp) pada Ikan Kerapu Cantang (Epinephelus fuscoguttatus X Epinephelus lanceolatus). Jurnal Lutjanus, 28(1), 40-47. https://doi.org/10.51978/jlpp.v28i1.511

Wynn, T. A., & Vanella, K. M. (2016). Machrophages in Tissue Repair, Regeneration, and Fibrosis. Journal of Immunity, 44(3), 450-462. https://doi.org/10.1016/j.immuni.2016.02.015




DOI: https://doi.org/10.33394/bioscientist.v12i1.10575

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