Propagasi In Vitro Kaliandra Merah (Calliandra calothyrsus Meisn.) II: Induksi Perakaran Tunas dan Aklimatisasi Planlet

Muhammad Idris, Auzia Asman, Deni Sorel, Edi Joniarti, Ulfa Mohtar, Harmailis Harmailis, John Nefri, Salvia Salvia

Abstract


Red calliandra is one of woody plants that has been extensively developed as biofuel. Tissue culture techniques are employed to produce a large and uniform quantity of seedlings in a relatively short period. Previous stages of in vitro initiation and multiplication of shoot from calliandra explants have shown promising results. The shoots that were multiplied by in vitro technique need to be induced for rooting process to produce plantlets. These plantlets are then prepared for the subsequent acclimatization process. This research was conducted in two stages; root induction of shoots using a modification of MS half-strength media (MS ½) supplemented with 1-4 mg/L 1-naphthaleneacetic acid (NAA), and acclimatization of the plantlets into soil media. The results shown that MS ½ medium and media supplemented with 1 mg/L NAA were suitable for root induction, particularly to induce the root length. Furthermore, MS ½ media supplemented with 1-2 mg/L NAA stimulates the increment of plantlet height and leaves numbers. Plantlets which were transferred into soil exhibit robust growth during 3-weeks of acclimatization process, up to 100% plantlets survived during this stage. These results underscore the potential of tissue culture techniques in providing large scale calliandra seedlings production for future biofuel development.


Keywords


Shoots, Root Induction, 1-Naphthaleneacetic Acid (NAA), Plantlet, Acclimatization.

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Adriyani, F., Utami, E. S. W., Purnobasuki, H., & Paramita, S. A. (2020). Development and Regeneration of Somatic Embryos from Leaves-Derived Calli of Coffea liberica. Biodiversitas : Journal of Biological Diversity, 21(12), 5829-5834. https://doi.org/10.13057/biodiv/d211246

Adugna, A. Y., Feyissa, T., & Tasew, F. S. (2020). Optimization of Growth Regulators on In Vitro Propagation of Moringa stenopetala from Shoot Explants. BMC Biotechnology, 20(1), 1-10. https://doi.org/10.1186/s12896-020-00651-w

Amghar, I., Ibriz, M., Ibrahimi, M., Boudra, A., Gaboun, F., Meziani, R., Iraqi, D., Mazri, M. A., Diria, G., & Abdelwahd, R. (2021). In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization. Plants, 10(6), 1-15. https://doi.org/10.3390/plants10061062

Andana, D. S., Jannah, H., & Safnowandi, S. (2023). Pemanfaatan Bintil Akar Kacang Tanah (Arachis hypogaea) sebagai Pupuk Biologi untuk Pertumbuhan Bibit Cabai Rawit (Capsicum frutescens) dalam Upaya Penyusunan Petunjuk Praktikum Fisiologi Tumbuhan II. Biocaster : Jurnal Kajian Biologi, 3(1), 1-10. https://doi.org/10.36312/bjkb.v3i1.145

Bidabadi, S. S., & Jain, S. M. (2020). Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration. Plants, 9(6), 1-20. https://doi.org/10.3390/plants9060702

Borowska, I. S., Ukalska, J., Wojda, T., Sulkowska, M., & Klisz, M. (2020). Micropropagation and In Vitro Rooting of Robinia pseudoacacia L. Recalcitrant Genotypes. Folia Forestalia Polonica, 62(1), 13-21. https://doi.org/10.2478/ffp-2020-0002

Danu., Aminah, A., Yuniarti, N., Syamsuwida, D., Cahyono, D. D. N., Siregar, N., Nugraheni, Y. M. M. A., & Hendarto, K. A. (2020). Keragaman Genetik Bibit Kaliandra (Calliandra calothyrsus Meissn.) Asal Jawa Barat. Jurnal Perbenihan Tanaman Hutan, 8(12), 121-132. https://doi.org/10.20886/bptpth.2020.8.2.121-132

do Vale, P. A. A., Junior, J. B. d. O., Costa, F. H. d. S., & Pereira, J. E. S. (2019). Height and Number of Shoots on The Survival and Development of Micropropagated Bamboo Plantlets during Pre-Acclimatization. Pesquisa Agropecuaria Tropical, 49(1), 1-8. https://doi.org/10.1590/1983-40632019v4953751

El-Fadl, A., Reda, E., Ahmad, M. E., & Alhady, M. R. A. A. (2022). In Vitro Propagation of Avocado (Persea americana Mill.). The Egyptian Journal of Desert Research, 72(1), 73-87. https://doi.org/10.21608/ejdr.2022.133868.1102

Fatimah, N. S., Arifin, Y. F., & Naemah, D. (2023). Analisis Perkembangan Tumbuh Tanaman Kaliandra Merah (Calliandra calothyrsus) di Area Pasca Tambang Tarjun Kotabaru. Jurnal Sylva Scienteae, 6(6), 1051-1055. https://doi.org/10.20527/jss.v6i6.11038

Florenika, N., Wijaya, A. N., Restanto, D. P., & Hardjo, P. H. (2022). Regeneration of Pogostemon cablin Benth. ‘Sidikalang’ through Indirect Organogenesis and Shoot Multiplication for Production of True-to-Type Plant. Agriprima : Journal of Applied Agricultural Sciences, 6(1), 1-11. https://doi.org/10.25047/agriprima.v6i1.447

Frick, E. M., & Strader, L. C. (2018). Roles for IBA-Derived Auxin in Plant Development. Journal of Experimental Botany, 69(2), 169-177. https://doi.org/10.1093/jxb/erx298

Harahap, F., Poerwanto, R., Suharsono., Suriani, C., & Rahayu, S. (2014). In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium with Different Concentrations of Plant Growth Regulator. Hayati : Journal of Biosciences, 21(4), 151-158. https://doi.org/10.4308/hjb.21.4.151

Hesami, M., & Daneshvar, M. H. (2018). In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-Derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant. HortScience, 53(1), 55-61. https://doi.org/10.21273/HORTSCI12637-17

Irsyadi, M. B. (2021). Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage. In Proceeding International Conference on Science and Engineering (pp. 100-104). Yogyakarta, Indonesia: Universitas Islam Negeri Sunan Kalijaga.

Jayusman., Hakim, L., & Dalimunthe, A. (2022). Season, Basal Media and Plant Growth Regulators Effect in Wood Plant In Vitro Propagation: A Comprehensive Review. IOP Conference Series : Earth and Environmental Science, 1115(1), 1-11. https://doi.org/10.1088/1755-1315/1115/1/012051

Kaur, K., Dolker, D., Behera, S., & Pati, P. K. (2022). Critical Factors Infuencing In Vitro Propagation and Modulation of Important Secondary Metabolites in Withania somnifera (L.) Dunal. Plant Cell, Tissue, and Organ Culture, 149(1), 41-60. https://doi.org/10.1007/s11240-021-02225-w

Li, H., Wang, H., Guan, L., Li, Z., Wang, H., & Luo, J. (2023). Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar. Life, 13(9), 1-12. https://doi.org/10.3390/life13091896

Listiana, B. E. (2018). Induksi Regenerasi In Vitro pada Spesies Tanaman Penghasil Gubal Gaharu, Aquilaria filaria. Crop Agro : Jurnal Ilmiah Budidaya, 10(1), 1-8.

Liu, J., Feng, H., Ma, Y., Zhang, L., Han, H., & Huang, X. (2018). Effects of Different Plant Hormones on Callus Induction and Plant Regeneration of Miniature Roses (Rosa hybrida L.). Horticulture International Journal, 2(4), 201-206. https://doi.org/10.15406/hij.2018.02.00053

Madke, S. S., Cherian, K. J., & Badere, R. S. (2014). A Modified Murashige and Skoog Media for Efficient Multipleshoot Induction in G. arborea Roxb. Journal of Forestry Research, 25(3), 557-564. https://doi.org/10.1007/s11676-014-0449-y

Maulana, A. F., Utomo, S., Lestari, L., Arifriana, R., Susanto, D., Dewi, N. A. C., Nugroho, A., Prasetyo, E., Pramono, R. F., Saputro, W. C., & Sulistyowati, D. (2021). Potensi Kaliandra (Calliandra calothyrsus) dan Gamal (Gliricidia sp.) di Daerah Istimewa Yogyakarta untuk Pengembangan Pelet Kayu. Agrifor : Jurnal Ilmu Pertanian dan Kehutanan, 20(1), 71-80. https://doi.org/10.31293/agrifor.v20i1.4924

Maulidani, A., Hatta, G. M., & Arifin, Y. F. (2019). Studi Daya dan Kualitas Hidup Kaliandra Merah (Calliandra calothyrsus) pada Tiga Jenis Tanah di Areal Reklamasi Bekas Penambangan Semen. Jurnal Sylva Scienteae, 2(3), 540-547. https://doi.org/10.20527/jss.v2i3.1834

Mohammed, M., Munir, M., & Ghazzawy, H. S. (2023). Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets. Agronomy, 13(1), 1-24. https://doi.org/10.3390/agronomy13010078

Monney, M. A. D., Amissah, N., & Blay, E. (2016). Influence of BA and IBA or NAA Combinations on Micropropagation of Cryptolepis sanguinolenta. American Journal of Plant Sciences, 7(3), 572-580. http://dx.doi.org/10.4236/ajps.2016.73050

Mudoi, K. D., Gogoi, B., Borah, G., Hussain, M., Tasfa, T., Borah, K., Lekhak, H., & Saikia, S. P. (2023). An Assessment for In Vitro Propagation and Genetic Stability of Phoebe goalparensis Hutchinson, An Endemic Valuable Timber Tree of North East India. Journal of Genetic Engineering and Biotechnology, 21(1), 1-10. https://doi.org/10.1186/s43141-023-00487-9

Mukasyaf, A. A., Faridah, E., Indrioko, S., & Herawan, T. (2017). Induksi Tunas, Multiplikasi dan Perakaran Gyrinops versteegii (Gilg.) Domke secara In Vitro. Jurnal Penelitian Tanaman Hutan, 11(1), 1-13. https://doi.org/10.20886/jpth.2017.11.1.1-13

Noorrohmah, S., & Imelda, M. (2015). Root Induction and Acclimatization of In Vitro Plantlets of Artocarpus altilis. In Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia (pp. 1896-1899). Surakarta, Indonesia: Universitas Sebelas Maret.

Nuroniah, H. S., & Bogidarmanti, R. (2020). In Vitro Propagation of White Mahang (Macaranga hypoleuca (Reichb.f.et Zoll.) Mull Arg.). IOP Conference Series: Earth and Environmental Sciencee, 533(1), 1-12. https://doi.org./10.1088/1755-1315/533/1/012030

Perez, L. P., Montesinos, Y. P., Olmedo, J. G., Sanchez, R. R., Montenegro, O. N., Rodriguez, R. B., Ribalta, O. H., Escriba, R. C. R., Daniels, D., & Kosky, R. G. (2015). Effects of Different Culture Conditions (Photoautotrophic, Photomixotrophic) and The Auxin Indole-Butyric Acid on the In Vitro Acclimatization of Papaya (Carica papaya L. var. Red Maradol) Plants Using Zeolite as Support. African Journal of Biotechnology, 14(35), 2622-2635. http://dx.doi.org/10.5897/AJB2015.14814

Prasetyo, E., Wiyono., Lestari, P., Hidayat, R., Oktalina, S. N., Ngadianto, A., & Nugroho, P. (2018). Penanaman Kaliandra sebagai Kayu Energi dan Hijauan Makanan Ternak pada Pertanaman Agroforestri Masyarakat Desa Gerbosari, Samigaluh Kulon Progo. Jurnal Pengabdian dan Pengembangan Masyarakat, 1(1), 1-10. https://doi.org/10.22146/jp2m.39216

Przybyl, T. H., Ratajczak, E., Obarska, A., & Kamczyc, E. P. (2020). Different Roles of Auxins in Somatic Embryogenesis Efficiency in Two Picea Species. International Journal of Molecular Sciences, 21(9), 1-22. https://doi.org/10.3390/ijms21093394

Purmadewi, G. C., Wulandari, A. S., & Damayanti, R. U. (2019). Pengaruh Metode Pengakaran dan Media Aklimatisasi terhadap Keberhasilan Aklimatisasi Tembesu (Fagraea fragrans (Roxb.) Miq.). Jurnal Perbenihan Tanaman Hutan, 17(1), 1-12. http://doi.org/10.20886/bptpth.2019.7.1.1-12

Putri, A. I., Kartikawati, N. K., Nirsatmanto, A., Sunarti, S., Haryjanto, L., Herawan, T., Santosa, P. B., Wahyuningtyas, R. S., Lestari, F., & Rimbawanto, A. (2023). Tissue Culture of Gerunggang (Cratoxylum arborescens (Vahl) Blume): Multipurpose Native Species of Indonesian Peatland. Forest Science and Technology, 19(3), 171-178. https://doi.org/10.1080/21580103.2023.2220010

Rahman, M. M., Ivy, N. A., Mian, M. A., Rasul, M. G., Hossain, M. M., & Rahman, M. A. (2018). Effect of Auxin (NAA, IBA and IAA) in Root Regeneration through In Vitro Culture of Sugarcane. International Journal of Plant Biology & Research, 6(6), 1-6. https://doi.org/10.47739/2333-6668/1109

Rezali, N. I., Sidik, N. J., Saleh, A., Osman, N. I., & Adam, N. A. M. (2017). The Effects of Different Strength of MS Media in Solid and Liquid Media on In Vitro Growth of Typhonium flagelliforme. Asia Pacific Journal of Tropical Biomedicine, 7(2), 151-156. https://doi.org/10.1016/j.apjtb.2016.11.019

Sharma, B., & Tanti, B. (2017). In Vitro Regeneration of Plantlets from Nodal Explants of Aristolochia saccata and Aristolochia cathcartii. European Journal of Biological Research, 7(3), 191-201. http://dx.doi.org/10.5281/zenodo.825746

Shekhawat, M. S., & Manokari, M. (2018). In Vitro Multiplication, Micromorphological Studies and Ex Vitro Rooting of Hybanthus enneaspermus (L.) F. Muell. - A Rare Medicinal Plant. Acta Botanica Croatia, 77(1), 80-87.

Siddique, I., Bukhari, N. A. W., Perveen, K., & Siddiqui, I. (2015). Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl. Brazilian Archives of Biology and Technology, 58(5), 686-691. https://doi.org/10.1590/S1516-89132015050290

Singh, K. K., & Singh, S. P. (2018). A Review: Micropropagation of Guava (Psidium spp.). Horticulture International Journal, 2(6), 462-467. https://doi.org/10.15406/hij.2018.02.00097

Song, J. Y., Bae, B., Lee, W., Lee, J. R., & Yoon, M. S. (2022). In Vitro Root Induction from Shoot Explants of Pear (Pyrus spp.). Korean Journal of Plant Resources, 35(6), 770-777. https://doi.org/10.7732/kjpr.2022.35.6.770

Sulistiyono, S. T. (2023). Retrieved January 11, 2024, from Tribun News. Interactwebsite: https://www.tribunnews.com/bisnis/2023/05/23/tekan-emisi-karbon-pohon-kaliandra-merah-bisa-jadi-bahan-bakar-alternatif-pengganti-batu-bara

Tamyiz, M., Prayoga, L., Prasetyo, R., Murchie, E. H., & Sugiyono. (2022). Improving Agarwood (Aquilaria malaccensis Lamk.) Plantlet Formation Using Various Types and Concentrations of Auxins. Caraka Tani : Journal of Sustainable Agriculture, 37(1), 142-151. https://doi.org/10.20961/carakatani.v37i1.58370

Warfa’ni, I., Prayoga, L., Prasetyo, R., Murchie, R. H., & Sugiyono. (2022). The Effect of Media Types and NAA Concentrations on Agarwood (Aquilaria malaccensis Lamk.) Shoot Development in In Vitro Culture. AgriHealth : Journal of Agri-Food, Nutrition and Public Health, 3(1), 62-71. https://doi.org/10.20961/agrihealth.v3i1.60346

Wudali, S., Nagella, P., Al-Khayri, J. M., & Jain, S. M. (2022). Role of Plant Tissue Culture Medium Components. In Rai, A. C., Kumar, A., Modi, A. & Singh, M. (Eds) Advances in Plant Tissue Culture, Current Developments and Future Trends (pp. 51-83). Oxford: Academic Press.

Yasmin, S., Hasan, M. J., Hossain, M. S., Saha, S., & Khatun, F. (2022). Auxin and Cytokinin Synergism in Micropropagation for Mass Production of Aloe vera. BioTechnologia : Journal of Biotechnology, Computational Biology, and Bionanotechnology, 103(3), 301-310. https://doi.org/10.5114%2Fbta.2022.118672

Yunita, R., & Nugraha, M. F. I. (2021). Effect of Auxin Type and Concentration on The Induction of Alternanthera Reineckii Roots In Vitro. IOP Conf. Series : Earth and Environmental Science, 653(1), 1-4. https://doi.org/10.1088/1755-1315/653/1/012073

Zhao, Y., Chen, Y., Jiang, C., Lu, M. Z., & Zhang, J. (2022). Exogenous Hormones Supplementation Improve Adventitious Root Formation in Woody Plants. Frontiers in Bioengineering and Biotechnology, 10(1), 1-6. https://doi.org/10.3389/fbioe.2022.1009531




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